[논문]Mechanism of Enzymatic Degradation of Poly(butylene succinate)

Lee, Chan-Woo; Kimura, Yoshiharu; Chung, Jin-Do

doi:10.1007/bf03218575

Mechanism of Enzymatic Degradation of Poly(butylene succinate) 원문보기

Macromolecular research, v.16 no.7, 2008년, pp.651 - 658

Lee, Chan-Woo (Department of Innovative Industrial Technology, Hoseo University) , Kimura, Yoshiharu (Department of Polymer Science and Engineering, Kyoto Institute of Technology) , Chung, Jin-Do (Department of Environmental Engineering, Hoseo University)

Abstract ▼ AI-Helper

Poly(butylene succiate) (PBS), poly(butylene succinate-co-L-lactate) (PBSL), and poly(butylene succinate-co-6-hydroxycaproate) (PBSCL) polymers were degraded by lipase $PS^{(R)}$, and the enzymatic degradation mechanism of PBS was analyzed in detail. The enzymatic degradation of PBS gave 4-hydroxybutyl succinate (4HBS) as the main product. An exo-type hydrolysis mechanism was proposed based on this observation. The terminal chain of PBS had conformational similarity to ordinary tri- and diglycerides and could be incorporated as a substrate in the active site of this lipase. The surface adsorption of the lipase was much larger on PBS and its copolymer films than on the other polyester films because the lipase adhered quite strongly to the polymer terminal through a specific adsorption mechanism. Kinetic analysis showed that the total number of surface adsorption points per unit area of PBSL and PBSCL copolymers was larger than that of the PBS homopolymer.

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200 MHz !H NMR spectra were measured on a Varian Gemini-200 spectrometer in CDC₁₃ and D2O with tetramethylsilane (TMS) and sodium 3-(trimethylsilyl)-1- propanesulfbnate (DSS) as the internal references, respectively. HPLC was conducted with a system composed of two Shimadzu LC-10A pumps and a Shimadzu RID-10A refractive index detector using a reversed phase column of COSMOSⅡ 5Ci8-MS (4.6 mm and 150 mm length; Nacalai Tesque). 0.
The formation of 4HBS could readily be monitored by the increase in UV absorption at 201 run to obtain the time course of the degradation ofPBS and PBS copolymers. The data were analyzed by the ordinary simple kinetic model, in which the enzyme is first adsorbed by the Langmuir-type mechanism, and the adsorbed enzyme hydrolyzes PBS to 4HBS by the exo mechanism. The whole reactions are shown in eq.

대상 데이터

The molecular weights (Mn and A#)and its distribution (MJMe were determined by gel permeation chromatography (GPC). The analyzer was composed of a Shimadzu LC-lOAd pump, a Shodex RI SE-31 RI detec- torm and a Shimadzu C-R7A Chromatopac data processor. A combination of two polystyrene gel columns of Tosoho TSK gel G4000H and G2500H (7.

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