[논문]ISSR에 의한 한국 내 원추리속 식물의 유전적 및 계통학적 연구

최주수; 허홍욱; 이설아; 허만규

doi:10.5352/jls.2008.18.6.753

[국내논문] ISSR에 의한 한국 내 원추리속 식물의 유전적 및 계통학적 연구
Genetic and Phylogenetic Relationships of Genus Hemerocallis in Korea Using ISSR 원문보기

생명과학회지 = Journal of life science, v.18 no.6 = no.98, 2008년, pp.753 - 758

최주수 (동의대학교 분자생물학과) , 허홍욱 (부산대학교 생물교육학과) , 이설아 (부산대학교 생물교육학과) , 허만규 (동의대학교 분자생물학과)

초록
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원추리속(Genus Hemerocallis) 식물은 초본이며 일부 종은 약용으로 매우 중요하다. 이 속내 8개 분류군에 대해 ISSR (inter simple sequence repeats) 마커로 계통학적 관계를 분석하였다. 조사한 식물은 원추리(Hemerocallis fulva), 왕원추리(H. fulva for. kwanso), 각시원추리(H. dumortieri), 골잎원추리(H. coreana), 홍도원추리(H. hongdoensis), 큰원추리(H. middendorffi), 노랑원추리(H. thunbergii), 애기원추리(H. minor)이다. 또한 이들 분류군에 대한 유전적 변이와 구조를 조사하였다. 종간 유전적 다양도는 $0.068{\sim}0.123$이며 평균 유전적 다양도는 0.098로 전반적으로 낮았다. 애기원추리가 가장 높은 값을 나타내었다. 세대 당 이주하는 개체수는 매우 적었다(Nm=0.128). 원추리속 종은 계통도에서 세 분지군으로 나누어졌다. 한 그룹은 H. fulva, H. fulva for. kwanso, H. middendorffi였다. 다른 그룹은 Hemerocallis, H. dumortieri, H. thunbergii, H. minor였다. 나머지는 H. coreana와 H. hongdoensis로 두 번째 그룹과 자매군을 형성하였다. 비록 종 내 적은 개체수로 분석하였지만 원추리속 식물종이 ISSR 마커로 잘 분리되었다.

Abstract ▼ AI-Helper

Genus Hemerocallis is a herbaceous species and some species among their taxa are very important herbal medicines. We evaluated representative samples of the eight taxa in Korea with inter simple sequence repeats (ISSR) markers to estimate phylogenetic relationships within taxa of this genus. The studied taxa were Hemerocallis fulva L., H. fulva for. kwanso, H. dumortieri Morren, H. coreana Nakai, H. hongdoensis M.G.Chung & S.S.Kang, H. middendorffi Trautv. et Mayer, H. thunbergii Baker, H. minor Miller. In addition, we investigated the genetic variation and structure of Korean populations of these taxa. The mean genetic diversity was 0.098 across species, varying from 0.068 to 0.123. A low level of genetic variation was found in populations of Hemerocallis species. Specially, gene diversity for H. minor was maintained the highest among genus Hemerocallis. An indirect estimate of the number of migrants per generation (Nm=0.218) indicated that gene flow was not extensive among Korean populations of Hemerocallis species. The phylogenic tree showed distinct three clades. One includes H. fulva, H. fulva for. kwanso and H. middendorffi. Another includes three Hemerocallis species, H. dumortieri, H. thunbergii and H. minor. The H. coreana and H. hongdoensis were shown as the sister group to the second clades. Although the size of sampling was not large enough for eight Korean Hemerocallis species, the analyses of ISSRs will certainly provide an enhanced view on the phylogeny of species.

주제어

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이론/모형

A phenetic relationship was constructed by the neighborjoining (NJ) method [21] using the NEIGHBOR program in PHYLIP version 3.57 [6].

성능/효과

From the eleven decamer primers used for a preliminary ISSR analysis’ six primers of them produced good amplification products for eight taxa of genus Hemerocallis in quality and variability, while the remaining primers (ISSR-01z ISSR-02, 1SSR-03, ISSR-07, ISSR-08, ISSR-09, ISSR-10, ISSR42) did not amplified or showed smear banding patterns (Table 2). For eight taxa of genus Hemerocallis, the primers ISSR-04 and ISSR-05 revealed the seven and nine distinct fragments, respectively.
Total genetic diversity values (Ht) varied between 0.069 for H. thunbergii and 0.176 H. dumorfieri, for an average over all polymorphic loci of 0, 323 (Table 4). Interlocus variation in the within-population genetic diversity (Hs) was low (0.
(Table 5). The genetic similarities between species ranged from a minimum value of 0.568 between H, fulva and H. hongdoensis and the maximum value of 0.991 between H. fulva and H. fulva for. kwanso.
amplified polymorphic DNA) [9]. Populations of the three species, H. thunbergii, H. middendorffii, and H. ex~ altata from Japan maintain high levels of allozyme variation: mean expected heterozygosities were 0.288z 0209, and 0.169, respectively. In this study, mean heterozygosities for eight species was 0.
numbers are mostly 2n드22. H. dumortieri, H. coreana, H. hongdoensis, H. middendorffi, H. thunbergii, and H. minor are all 2m=22. While H.
hongdoensis). Second, the reproductive isolation among species on inland is in a primary condition, and the stronger isolation is developed among Korean populations of several species in genus Hemerocallis. H.

후속연구

In summary, ISSR amplifications is a simple and effective meth-od/that can be used in the identification and intraspecific differentiation of genus Hemerocallis with a high degree of discrimination and reproducibility. In addition, additional molecular experiments such as AFLP (amplified fragment length polymorphism), microsatellites, and ITS (nuclear ribosomal DNA internal transcribed spacer sequences) are necessary to identify species.

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