This paper describes a pattern recognition method of Magnoliae flos based on a gas chromatographic/mass spectrometric (GC/MS) analysis of the essential oil components. The botanical drug is mainly comprised of the four magnolia species (M. denudata, M. biondii, M. kobus, and M. liliflora) in Korea, ...
This paper describes a pattern recognition method of Magnoliae flos based on a gas chromatographic/mass spectrometric (GC/MS) analysis of the essential oil components. The botanical drug is mainly comprised of the four magnolia species (M. denudata, M. biondii, M. kobus, and M. liliflora) in Korea, although some other species are also being dealt with the drug. The GC/MS separation of the volatile components, which was extracted by the simultaneous distillation and extraction (SDE), was performed on a carbowax column (supelcowax 10; 30 m{\time}0.25 mm{\time}0.25{\mu}m$) using temperature programming. Variance in the retention times for all peaks of interests was within RSD 2% for repeated analyses (n = 9). Of the 74 essential oil components identified from the magnolia species, approximately 10 major components, which is $\alpha$-pinene, $\beta$-pinene, sabinene, myrcene, d-limonene, eucarlyptol (1,8-cineol), $\gamma$-terpinene, p-cymene, linalool, $\alpha$-terpineol, were commonly present in the four species. For statistical analysis, the original dataset was reduced to the 13 variables by Fisher criterion and factor analysis (FA). The essential oil patterns were processed by means of the multivariate statistical analysis including hierarchical cluster analysis (HCA), principal component analysis (PCA) and discriminant analysis (DA). All samples were divided into four groups with three principal components by PCA and according to the plant origins by HCA. Thirty-three samples (23 training sets and 10 test samples to be assessed) were correctly classified into the four groups predicted by PCA. This method would provide a practical strategy for assessing the authenticity or quality of the well-known herbal drug, Magnoliae flos.
This paper describes a pattern recognition method of Magnoliae flos based on a gas chromatographic/mass spectrometric (GC/MS) analysis of the essential oil components. The botanical drug is mainly comprised of the four magnolia species (M. denudata, M. biondii, M. kobus, and M. liliflora) in Korea, although some other species are also being dealt with the drug. The GC/MS separation of the volatile components, which was extracted by the simultaneous distillation and extraction (SDE), was performed on a carbowax column (supelcowax 10; 30 m{\time}0.25 mm{\time}0.25{\mu}m$) using temperature programming. Variance in the retention times for all peaks of interests was within RSD 2% for repeated analyses (n = 9). Of the 74 essential oil components identified from the magnolia species, approximately 10 major components, which is $\alpha$-pinene, $\beta$-pinene, sabinene, myrcene, d-limonene, eucarlyptol (1,8-cineol), $\gamma$-terpinene, p-cymene, linalool, $\alpha$-terpineol, were commonly present in the four species. For statistical analysis, the original dataset was reduced to the 13 variables by Fisher criterion and factor analysis (FA). The essential oil patterns were processed by means of the multivariate statistical analysis including hierarchical cluster analysis (HCA), principal component analysis (PCA) and discriminant analysis (DA). All samples were divided into four groups with three principal components by PCA and according to the plant origins by HCA. Thirty-three samples (23 training sets and 10 test samples to be assessed) were correctly classified into the four groups predicted by PCA. This method would provide a practical strategy for assessing the authenticity or quality of the well-known herbal drug, Magnoliae flos.
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제안 방법
GC/MS an시ysis. GC/MS analyses were performed with an Eun SookJeong et al Agilent 7890 series GC system coupled to an Agilent 5975B inert MSD (Agilent, CA, USA). A supelcowax 10 column (30 m x 0.
Method validation. To check the reproducibility, three QC samples (D-7, U-4 and U-8) were analyzed repeatedly three times per sample on three separate days (n = 9). Variance in the retention times for all peaks of interests was within RSD 2%.
대상 데이터
salicifolia Daejeon, Korea U-1 herbal drug Seoul, Korea U-2 herbal drug Daegu, Korea U-3 herbal drug Seoul, Korea U-4 herbal drug Seoul, Korea U-5 herbal drug Seoul, Korea U-6 herbal drug Seoul, Korea U-7 herbal drug Youngcheon, Korea U-8 herbal drug Youngcheon, Korea U-9 herbal drug Youngcheon, Korea U-10 plant sample Daegu, Korea U-11 plant sample Daegu, Korea U-12 plant sample Daegu, Korea U-13 plant sample Daegu, Korea U-14 plant sample Daegu, Korea U-15 plant sample Daegu, Korea U-16 plant sample Daegu, Korea analytical grade quality and were redistilled before use. Anhydrous sodium sulfate was purchased from Samchun Chemical (Pyeongtak, Korea). Sample pnepaiation.
salicifolia) were collected from Korea and China during March to April 2008. The 20 reference specimens identified were used. The 9 drugs (U-1 〜U-9) were purchased from oriental herbal stores in Korea, and 7 samples (U-10 〜U-16) were obtained fromDaegu Catholic University, and dried under air prior to analysis.
The 20 reference specimens identified were used. The 9 drugs (U-1 〜U-9) were purchased from oriental herbal stores in Korea, and 7 samples (U-10 〜U-16) were obtained fromDaegu Catholic University, and dried under air prior to analysis. (Table 1) Chemic시s.
Experimental Section Plant materials. Twenty specimens ofM.flos (3 M. biondii, 8 M. denudata, 3 M. kobus, 3 M. liliflora, M. denudata var. purpurascens, M. liliflora var. gracilis andM. salicifolia) were collected from Korea and China during March to April 2008. The 20 reference specimens identified were used.
이론/모형
, Chicago, USA). The HCA was performed by Ward’s method using squared Euclidian distance as a measure of similarity. For PCA analysis, the eigenvalues of > 1.
성능/효과
The score plot of the first three principal components (Figure 2) showed the clear differentiation of the species. From the scatter points, the samples could be classified into four groups, which were marked as group I-IV according to the species: M. biondii (Group I); M. denudata (Group II); M. kobus (Group III) and M. liliflora (Group IV). M.
참고문헌 (21)
Bae K. In Medicinal Plants of Korea; Kyu-Hak Press: Seoul, Korea, 2000
Bensky, D.; Gamble, A. In Chinese Herbal Medicine(Materia Medic); Eastland Press: Washington, U.S.A., 1993
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