[논문]Beneficial Effect of Anti-obese Herbal Medicine Mixture with Chitosan in High Fat Diet-induced Obese Rats

Beik, Kyung-Yeun; Lee, Sang-Il; Kim, Soon-Dong

doi:10.3746/jfn.2009.14.4.290

Beneficial Effect of Anti-obese Herbal Medicine Mixture with Chitosan in High Fat Diet-induced Obese Rats 원문보기

Journal of food science and nutrition, v.14 no.4, 2009년, pp.290 - 297

Beik, Kyung-Yeun (Department of Food Science & Technololgy, Faculty of Food Industrialogy Technology, Catholic University of Daegu) , Lee, Sang-Il (Department of Food Nutrition & Cookery, Keimyung College) , Kim, Soon-Dong (Department of Food Science & Technololgy, Faculty of Food Industrialogy Technology, Catholic University of Daegu)

Abstract ▼ AI-Helper

This study was carried out to investigate the dietary effects of chitosan mixture (CM), an herbal medicine mixture with Sukjihwang (HS), and CM containing HS (CHS) on obesity in an induced obese model of rats fed high-fat only (HF), in which supplemented diets of 5% CM (HCM), 5% HS (HHS), or 2.5% CM-2.5% HS (HCH) was tested for 6 weeks. Body weight gains, obesity indexes, and body fat contents in the experimental groups (HCM, HHS, HCH) were decreased compared with HF group. The levels of serum triglyceride, total lipid, total cholesterol and LDL-cholesterol in the experimental groups were markedly decreased, however HDL-cholesterol levels in the experimental groups were slightly increased compared with HF group. In addition, although serum ALT and AST activity, and relative organ weights were lower than those of HF group, serum albumin contents were not significantly different in all experimental groups including the normal control group (NC). In conclusion, there are improved effects on obesity in the obese model of animals with all experimental diets supplementations, and the improvement degrees on obesity depend on the content and compositions of the herbal medicine mixture. Further study is needed on the anti-obesity mechanism of these diets.

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가설 설정

2.

2) NS: not significant.
5% CM for 6 weeks. Abbreviations: See Table 2. Each value is mean 士 SD of experimental group, n = 6. Different alphabets in each values show statistically difference at a = 0.

제안 방법

The high-fat diet group was fed a standard pellet chow with an added 15% lard supplemented diet for 4 weeks for induction of obesity. After obesity was confirmed by checking obesity indexes, the animals were divided into 5 groups (n=6); first, the normal control (NC), and then taken from the obese model of rats, a high-fat diet obesity control group (HF), 5% chitosan mixture supplemented high-fat diet group (HCM), 5% herbal medicine mixture with Sukjihwang supplemented high-fat diet group (HHS), and 2.5% chitosan mixture plus 2.5% herbal medicine mixture with Sukjihwang supplemented high-fat diet group (HCH) for 6weeks as seen in Table 2. The rats were individually housed in stainless steel wire-bottom cages in a room maintained at 20 ± 2oC and 60 ±5% relative humidity.
2. Changes of serum aminotransferases (ALT & AST) activities, albumin and total protein contents in rats fed high fat diet with 5% chitosan mixture (CM), 5% herb medicine mixture with Sukjihwang (HS), and 2.5% HS plus 2.5% CM (HCM) for 6 weeks. Abbreviations: See Table 2.
In order to confirm the obese animal model, we observed the content of body fat, and the Rohrer, Lee and TM indexes for the obesity index in high-fat diet pretreated rats for 4 weeks. The Rohrer index (30.

데이터처리

2) Each value is mean士 SD of experimental group, n = 6. Different alphabets in each values show statistically difference at a = 0.05 by Duncan's multiple range test.
(SD) of the 6 animals. Statistical comparisons of differences between the different groups was carried out by doing a two-way ANOVA test followed by Duncan's multiple range test using SPSS statistical software package (Version 12.0, SPSS Inc., Chicago, IL, USA).

이론/모형

, Korea). And the content of LDL-cholesterol was calculated by using the method of Friedewald et al. (27). The atherogenic index (AI) was calculated from (total choles-terol-HDL-cholesterol)/ total-cholesterol].

성능/효과

Fig. 1. Changes of obesity indexes in rats fed high fat diet with 5% chitosan mixture (CM), 5% herb medicine mixture with Sukjihwang (HS), and 2.5% HS plus 2.5% CM for 6 weeks. Abbreviations: See Table 2.
It is also reported that relative liver weight was increased in rats fed the high-fat diet (52, 53). Considering the reports, all experimental diets could modulate accumulation of lipid as well as metabolic functions in active metabolic organs such as the liver, heart and kidney in cases where high-fat diets are consumed and without severe organ toxicities.
3. Final body weight and weight gain in all experimental diet groups (HCM, HHS & HCH) were decreased 7.2 ~10.5% and 10.2 ~27.4% respectively, when compared with the HF group after 6 weeks. However, feed, calorie intake, and FER in experimental groups were not significantly different from the HF group (Table 3).
In conclusion, although the exact mechanisms of the anti-obesity effects of the experimental diets are unknown, the results provide a basis for developing efficacious healthy food or medicines for anti-obesity using chitosan and traditional herbs such as Rehmanniae Radix, Coicis Semen, Eucommia Cortex, Atractylodes Rhizoma Alba, and Gingseng Radix. Further study is needed to elucidate the anti-obesity mechanism of these diets.
Generally, it is known that obese indexes and serum lipid profiles were decreased by hypolipidemic materials (47). In this study, obese indexes and serum lipid profiles such as TG, total cholesterol and LDL-cholesterol were decreased by all experimental diets. These results suggest that all experimental diets in this study showed anti-obese and hypolipidemic effects.
are shown in Table 4. Levels of serum triglyceride, total lipid, total cholesterol, LDL-cholesterol and the atherogenic index in experimental diet groups were significantly decreased with levels of 46.0~51.1%, 13.0 ~ 19.8%, 11.7~26.1%, 52.0~65.0% and 31.7~51.7% compared with HF group, respectively. In addition, HDL-cholesterol levels in experimental groups were increased by 28.
The results of this study indicate that obesity was improved by the experimental diets such as chitosan mixture (CM), herbs mixture with Sukjihwang (HS), and CM plus HS (CH), especially it was considered that the mixed diet of herbs-chitosans (CH) could have synergistic effects on obesity.

후속연구

Alba, and Gingseng Radix. Further study is needed to elucidate the anti-obesity mechanism of these diets.

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