[논문]운동훈련과 마늘가루 섭취에 의한 흰쥐의 혈중 지질 및 MDA, SOD 활성 차이

전병덕; 김진하; 류승필

doi:10.5352/jls.2009.19.10.1337

운동훈련과 마늘가루 섭취에 의한 흰쥐의 혈중 지질 및 MDA, SOD 활성 차이
Differences of Garlic Powder Ingestion and Exercise Training on Blood Lipids, MDA and SOD in Rats 원문보기

생명과학회지 = Journal of life science, v.19 no.10 = no.114, 2009년, pp.1337 - 1345

전병덕 (경북외국어대학교 관광스포츠경영학부) , 김진하 (경북대학교 생태환경대학 레저스프츠학과) , 류승필 (경북대학교 생태환경대학 레저스프츠학과)

초록
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본 연구는 운동훈련과 마늘가루 섭취가 혈중지질과 항산화활성에 미치는 영향을 조사한 것으로서 28마리의 SD계 수컷 흰쥐를 사용하여 고지방식이와 함께 마늘분(500 mg/kg)을 사료에 혼합시켜 섭취시켰다. 집단은 대조군(CON), 운동군(EXE), 마늘군(GAR), 그리고 마늘분과 운동훈련 병행군(GAREXE)로 각각 구분하였다. EXE와 GAREXE는 트레드밀 달리기를 4주동안 실시하였다. 지방량(장간막, 신주위, 부고환주위)의 정량과 혈중 글루코스, 중성지방(Triglyceride: TG), 총콜레스테롤(Total cholesterol: TC), 고밀도지단백콜레스테롤(High density lipoprotein cholesterol: HDL-C), 저밀도지단백콜레스테롤(Low density lipoprotein cholesterol: LDL-C) 등을 분석하였다. 간조직의 MDA (Malondialdehyde)와 SOD (Superoxide dismutase) 활성을 분석하였다. GAREXE의 체중은 다른 집단에 비하여 통계적으로 유의하게 낮은 것으로 나타났으며(p<0.05), GAR과 GAREXE이 경우에 지방량 역시 유의하게 낮은 것으로 나타났다(p<0.05). 혈중 글루코스는 CON이 다른 집단에 비하여 통계적으로 유의하게 높은 것으로 나타났으며 (p<0.05), TG는 GAR과 GAREXE가 다른 집단에 비하여 유의하게 낮은 것으로 나타났다. TC는 GAREXE가 CON 및 GAR에 비하여 통계적으로 유의하게 낮은 것으로 나타났으며(p<0.05), HDL-C는 CON에 비하여 EXE와 GAR에서 유의하게 개선된 것으로 나타났으며(p<0.05), GAREXE는 EXE에 비하여 유의하게 높은 농도를 나타내었다(p<0.05). MDA 함량은 GAREXE가 EXE에 비하여 유의하게 낮은 것으로 나타났으며(p<0.05), SOD 활성은 CON에 비하여 다른 집단에서 유의하게 높은 것으로 나타났다(p<0.05). GAREXE의 경우에 EXE와 GAR에 비하여 유의하게 높은 것으로 나타나 운동과 병행할 경우의 유의한 상승효과가 나타났다(p<0.05). 이러한 결과를 볼 때, 운동훈련을 실시하는 경우에 마늘분 섭취는 혈중 글루코스를 낮추고, 혈중지질을 개선하는데 효과가 있으며, 항산화효과 역시 나타났다고 생각된다. 추후 마늘의 추출법, 제조공정, 섭취방법 등에 대한 다양한 연구방법적 접근을 통해서 마늘이 가지고 있는 다양한 효과에 대한 검증이 필요하다고 사료된다.

Abstract ▼ AI-Helper

This study investigated the effect of exercise training and garlic powder ingestion on blood lipids and antioxidants activity in rats. Twenty-eight male Sprague Dawley rats were fed a high-fat diet with or without garlic powder (500 mg/kg) for four weeks as grouped in control (CON), exercise (EXE), garlic (GAR), and garlic + exercise training (GAREXE), respectively. EXE and GAREXE were trained on the treadmill for the same periods. Weight of fats (mesentery, perirenal, and epididymal) were weighed and blood glucose, triglycerides (TG), total cholesterol (TC), and high density lipoprotein- cholesterol (HDL-C) were analyzed and low density lipoprotein-cholesterol (LDL-C) was calculated. Malondialdehyde (MDA) and superoxide dismutase (SOD) for lipid peroxidation were analyzed in liver tissue. Body weight in GAREXE was significantly lower in the statistics than that in other groups (p<0.05), and the volume of fat in GAR and GAREXE was also much lower (p<0.05). Blood glucose was significantly lower in EXE and GAR (p<0.05), however, there was no effect of exercise training. Blood TG was lower in GAR and GAREXE (p<0.05), however, there was no effect of exercise training. HDL-C was significantly improved in EXE and GAR compared to CON (p<0.05), and GAREXE was higher than EXE (p<0.05). MDA content was considerably lower in GAREXE compared to EXE (p<0.05), and SOD activity was much higher in other groups compared to CON (p<0.05). In addition, GAREXE was significantly higher than EXE and GAR, thus there was significant increase when a garlic diet was carried out together with exercise (p<0.05). These results suggested that garlic powder ingestion during the training periods had a beneficial effect of lowering glucose and enhancing blood lipids profiles. Moreover, it also has antioxidant effects, which means that it could possibly suppress aging. It is necessary to inspect various effects of garlic with a variety of research methods regarding sampling process, production process, intake method, etc.

주제어

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문제 정의

Therefore, this study aims at inspecting the effect of garlic which can suppress lipid peroxides that can be generated when performing exercise along with the effect of reducing blood lipids. For this purpose this study makes rats take garlic powder, and suggests various results related to functional foods as natural food, not as chemical compound.

제안 방법

rest, smoking, drinking, and so on [19]. This study tried to examine the influence of performing exercise training and taking garlic powder for 4 weeks on blood lipids and lipid peroxides.

대상 데이터

For this study, 28 male ratsz aged five-week of SD (Sprague-Dawley) were purchased from Hyochang Science (Korea) as the subjects. The rats of the same age and the weight from 120 to 140 g were purchased, and preliminary breeding had been carried out for two weeks before group classification.

데이터처리

z USA) for Windowsz and the data were represented by mean 土 standard error of mean (SEM). The one-way ANOVA was used in order to compare all the variables of each groupz and tukey test was performed as post-hoc when there was significant difference. The sig- nificance level of all the differences was p<0.

이론/모형

MDA was analyzed by using the method presented by Saitoh [38]. 0.
Rats underwent restrict diet by 10 g of feed in the morning and in the afternoon for providing identical calories. The high-fat diet used in this study (C: 43% F: 40% P: 17%) was made the basis of the tables of food composition formula (AIN-76). The rats were divided into the control group (CON), the exercise training group (EXE), the garlic powder ingestion group (GAR), and the garlic powder ingestion and exercise training group (GAREXE).

성능/효과

05). As for LDL-CZ when compared to that of the CONZ that of the EXE, the GAR, and the GAREXE was significantly restrained (p<0.05). And synergic effects were found in the GAREXE on blood TG and HDL-C (p<0.
However, they reported that there was no significant difference in case of HDL-C. In this study, however, unlike previous studies above, there was no significant change in TC in the GAR, but it tended to be decreased, and there was a significant decrease in the GAREXE. It is considered to be caused by the fact that there was increase after taking garlic powder and exercise.
The CON was significantly high in glucose, which was significantly inhibited in the EXE, the GAR, and the GAREXE, respectively (p<0.05). Blood TG was shown to be inhibited significantly in the GAR and the GAREXE (p<0.

후속연구

Also, taking garlic for more than 6 months does not have continuous ef- feet of reducing TC and TGZ so the less than 6 months taking garlic might have the greater effect [2]. It is considered that there is an effect from short-term taking garlic powder also in this study, and it is necessary to research the effect of long-term case later. Furthermore, Abramoviz et al.

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