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가설 설정
- 1. Treatment with CCL4 increased liver lipid peroxidation to 41.7 ± 3.3 ㎍/g tissue compared with that (38.4 ± 2.3 ㎍/g tissue) of normal.
제안 방법
- Rats were acclimated to the environment for one week, used for the experiment in the conditions of 22~24℃ for the experiment period (2 weeks), and allowed tap water free. Experiment groups were composed of five groups such as normal, CCL4 (negative control), CCL4 plus silymarin (positive control), CCL4 plus Lythrum salicaria flower extract (LSF), and CCL4 plus Lythrum salicaria root extract (LSR) groups. Normal group was administered with basal diet and treated with intra-peritoneal (i.
- In the study, several plant parts of L. salicaria such as flower, aerial parts and root were preliminarily investigated and compared on their antioxidant activity against free radicals such as DPPH and superoxide and against oxidation of linoleic acid. In these in vitro assay, we obtained the result that flower and root of L.
대상 데이터
- Flower and root selected from in vitro assay were subjected to in vivo experiment. Animals for evaluating the activity of two parts of L. salicaria were Sprague Dawley male rats which were six week-old age and had about 200 g body weight at the start time of experiment, were divided randomly into six groups (n = 8). Rats were acclimated to the environment for one week, used for the experiment in the conditions of 22~24℃ for the experiment period (2 weeks), and allowed tap water free.
- Reagents including 2,2-diphenyl-1-picrylhydrazyl (DPPH), nitro blue tetrazolium (NBT), phenazine methosulfate (PMS), β-nicotine amide adenine dinucleotide (NADH), and silymarin were the product of Sigma-Aldrich Co (St. Louis, USA).
데이터처리
- The significance of data was verified with Duncan's multiple range test of one way ANOVA test in SAS program (Enterprise Guide 4) at p < 0.05.
이론/모형
- 0) for 5 min at room temperature, was measured on an absorbance at 421 ㎚ and the result was obtained from substitution of the absorbance to glutathione standard curve. Measurements of GOT activity and GTP activity in serum were performed with the GOT and GPT assay kit (Asan Pharmaceutical Co). Content of protein in cytosol fraction was determined by the method of Bradford (1976) using bovine serum albumin as standard compound for calibration curve.
- Glutathione (GSH) content in liver tissue was analyzed by the method of Ellman (1959) in which a part of liver tissue was homogenized and an aliquot of homogenate as 400~600 ㎍ of protein was mixed with 2 ㎖ of 4% sulfosalicylic acid, centrifuged 10 min at 3,000 rpm, and then, 0.1 ㎖ of the supernatant was reacted with 2.7 ㎖ of 0.1 mM 5,5'-dithiobis (2-nitrobenzoic acid) in 0.1 M sodium phosphate buffer (pH 8.0) for 5 min at room temperature, was measured on an absorbance at 421 ㎚ and the result was obtained from substitution of the absorbance to glutathione standard curve.
성능/효과
- 2. Activity of glutathione peroxidase (GSHpx) in NC rats decreased as 0.36 ± 0.15 Uk/㎎ protein compared with the activity of normal rats (0.67 ± 0.16 Uk/㎎ protein).
- Antioxidant activity of aerial part extract on linoleic acid oxidation tested at final reaction concentration of 10 ㎍/㎖, exhibited the highest value (82.7 ± 1.4%), and those of flower and root extracts showed 78.4 ± 2.1% and 76.2 ± 5.6%, respectively.
- As shown in Fig. 4, carbon tetrachloride intoxication in CCL4 single treated rats increased GPT activity (34.4 ± 8.1 U/L) compared with normal group (23.9 ± 2.1 U/L), but the activities in 1% L. salicaria flower extract and 1% L. salicaria root extract diet administered rats were decreased into 25.3 ± 4.0 U/L and 19.6 ± 4.4 U/L, which were significantly lower than that of 1% silymarin diet administrated rats (28.3 ± 4.8 U/L).
- In DPPH radical scavenging activity shown as IC50 in root extract of L. salicaria showed 7.7 ± 0.1 ㎍/㎖ which was the most effective among the extracts, and flower extract and aerial part extract showed 10.0 ± 0.0 ㎍/㎖ and 18.3 ± 1.1 ㎍/㎖, respectively.
- In conclusion, flower and root of L. salicaria showed in vitro antioxidant activities. Furthermore, flower and root selected from in vitro assay showed antioxidant and liver protecting activities on carbon tetrachloride intoxicated liver injury of rats.
- salicaria flower and root extract in rats intoxicated with carbon tetrachloride three times for 2 weeks. Supplement with CCL4 plus 1% L. salicaria root extract extract diet showed significantly protecting activity on hepatic lipid peroxidation (as TBARS) compared with CCL4 single treatment, CCL4 plus 1% L. salicaria flower extract diet administration and CCL4 plus 1% silymarin diet administration.
- Supplements with L. salicaria flower and root extract decreased serum GOT and GPT activities which increased in CCL4 singly administered rats compared with normal rats, and L. salicaria root extract showed more effective in decreasing GPT activity than silymarin did. These results demonstrate that L.
- The activity of hepatic Mn-SOD in CCL4 single treated rats (2.17 ± 0.10 U/㎎ protein) increased, and the activities in L. salicaria flower extract plus carbon tetrachloride treated rats (2.10 ± 0.18 U/㎎ protein), L. salicaria root extract plus CCL4 treated rats (2.01 ± 0.33 U/㎎ protein), and silymarin plus CCL4 treated rats (2.10 ± 0.47 U/㎎ protein) slightly decreased than that in normal rats (1.98 ± 0.19 U//㎎ protein), which were not significantly different among the groups.
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