L-ascorbic acid (Vitamin C) and peptide are both useful compounds for collagen biosynthesis in cosmeceuticals (cosmetic and pharmaceutical fields). The instability of these compounds, however, limit their application in these industries. In this report, we describe the development of a novel compoun...
L-ascorbic acid (Vitamin C) and peptide are both useful compounds for collagen biosynthesis in cosmeceuticals (cosmetic and pharmaceutical fields). The instability of these compounds, however, limit their application in these industries. In this report, we describe the development of a novel compound, Stabilized Ascorbyl Pentapeptide (SAP), which physically is much more stable than L-ascorbic acid in water. The inhibitory effects of this SAP compound on tyrosinase and melanin synthesis is comparable to that of L-ascorbic acid. Importantly, the SAP compound displays no cytotoxicity at a high concentration (5 mM). The ability of SAP to promote collagen biosynthesis is greater than that of L-ascorbic acid or the KTTKS peptide alone. Considering the in vitro stability and functional effects, our data strongly suggest that the SAP compound is a good candidate not only as a cosmetic ingredient, but also as a wound healing agent.
L-ascorbic acid (Vitamin C) and peptide are both useful compounds for collagen biosynthesis in cosmeceuticals (cosmetic and pharmaceutical fields). The instability of these compounds, however, limit their application in these industries. In this report, we describe the development of a novel compound, Stabilized Ascorbyl Pentapeptide (SAP), which physically is much more stable than L-ascorbic acid in water. The inhibitory effects of this SAP compound on tyrosinase and melanin synthesis is comparable to that of L-ascorbic acid. Importantly, the SAP compound displays no cytotoxicity at a high concentration (5 mM). The ability of SAP to promote collagen biosynthesis is greater than that of L-ascorbic acid or the KTTKS peptide alone. Considering the in vitro stability and functional effects, our data strongly suggest that the SAP compound is a good candidate not only as a cosmetic ingredient, but also as a wound healing agent.
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제안 방법
incubator for 72 hrs with various concentrations of test samples ranging from 1 uM to 5,000 uM to identify the cytotoxicity of test samples. Mitochondrial dehydrogenase activity was used as an index of cell viability and was evaluated using 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT, Sigma), which is reduced to an insoluble formazan dye. Culture media was replaced with fresh 0.
Next, the peptide was coupled with a succinoyl phosphoryl ascorbate derivative to generate SAP. The in vitro stability test was performed by dissolving 0.1 mg Vitamin C and SAP in distilled water at room temperature and analyzed by HPLC for 28 days.
데이터처리
Statistical significance was evaluated by using student’s t-test.
성능/효과
Results of the MTT assay indicate that the activity of mitochondrial dehydrogenases do not decrease following incubation of human dermal fibroblasts (HDF) with various concentrations (1 uM-5 mM) of L-Ascorbic acid or SAP for 72 hours (Supplemental Fig. 2).
후속연구
In conclusion, we have presented the first promising experimental evidence that ascorbic acid-conjugated peptide is a more stable useful cosmetic and pharmaceutical material in vitro and in vivo. Further studies will be focused on characterizing various formulations of our conjugate for their anti-aging and anti-wrinkle acivities through clinical applications.
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