Fifty seven species of common seaweed from the coast of Korea were screened for antifungal activity against Malassezia species. Seaweeds as a source of bioactive compounds are able to produce a great variety of secondary metabolites with different activities. There are numerous reports on the biolog...
Fifty seven species of common seaweed from the coast of Korea were screened for antifungal activity against Malassezia species. Seaweeds as a source of bioactive compounds are able to produce a great variety of secondary metabolites with different activities. There are numerous reports on the biological activities of seaweeds against human pathogens, fungi, and yeasts, but only few contain data regarding inhibitory effects against Malassezia sp., a major cause of dandruff and seborrheic dermatitis. To help address this paucity of information, this work was carried out to examine the antifungal effects of seaweed extracts against M. furfur and M. restricta. Of the fifty seven species of marine algae screened for their potential antifungal activity, only 17 species (29.8%) exhibited inhibitory activity. In agar disc diffusion method, the ether extracts of Corallina pilulifera, Enteromorpha linza, Laminaria japonica, Symphyocladia latiuscula and Ulva sp. showed strong antifungal activity. To identify major constituents in seaweed extracts, four selected extracts were analyzed on' a GC-MS equipped with a flame ionization detector, and compared to spectral data from databases WILEY229.LIB and NIST107.LIB. Most constituents in seaweed extracts are fatty acid-related compounds. When we evaluated any acute toxicity, the ether extracts of the selected four species were not toxic in mice. According to these results, it can be suggested that these seaweed extracts are valuable for the development of therapeutic agents in treating dandruff and seborrheic dermatitis. Further investigations to determine its bioactive compound(s) are currently in progress.
Fifty seven species of common seaweed from the coast of Korea were screened for antifungal activity against Malassezia species. Seaweeds as a source of bioactive compounds are able to produce a great variety of secondary metabolites with different activities. There are numerous reports on the biological activities of seaweeds against human pathogens, fungi, and yeasts, but only few contain data regarding inhibitory effects against Malassezia sp., a major cause of dandruff and seborrheic dermatitis. To help address this paucity of information, this work was carried out to examine the antifungal effects of seaweed extracts against M. furfur and M. restricta. Of the fifty seven species of marine algae screened for their potential antifungal activity, only 17 species (29.8%) exhibited inhibitory activity. In agar disc diffusion method, the ether extracts of Corallina pilulifera, Enteromorpha linza, Laminaria japonica, Symphyocladia latiuscula and Ulva sp. showed strong antifungal activity. To identify major constituents in seaweed extracts, four selected extracts were analyzed on' a GC-MS equipped with a flame ionization detector, and compared to spectral data from databases WILEY229.LIB and NIST107.LIB. Most constituents in seaweed extracts are fatty acid-related compounds. When we evaluated any acute toxicity, the ether extracts of the selected four species were not toxic in mice. According to these results, it can be suggested that these seaweed extracts are valuable for the development of therapeutic agents in treating dandruff and seborrheic dermatitis. Further investigations to determine its bioactive compound(s) are currently in progress.
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제안 방법
After 72 hrs of incubation the inhibition zone from the edge of the disc to the inner margin of the surrounding bacterial growth was measured in mm and recorded. Seaweed extracts that showed strong activity were further investigated to confirm the dose response of antifungal activity against M. furfur and M. restricta. Controls were also run simultaneously.
The aim of this study was to examine the anti fungal effects of seaweed extracts against M. furfur and M. restricts while attempting to develop natural therapeutic agents for dandruff and seborrheic der matitis that have no side effects and high antifungal activity.
To identify major constituents in seaweed extracts, the extracts were analyzed on a GC-MS-QP5050A (Shimadzu, Kyoto, Japan) equipped with a flame ionization detector, and compared to spectral data from databases WILEY229.LIB and NIST107.
대상 데이터
Fifty seven seaweed species (10 of Chlorophyta, 29 of Phaeophyta and 18 of Rhodophyta) were used in this study. Seaweed tissues were dried for 1 day at room temperature using an electric fan and then ground to powder using a coffee grinder for 10 min.
The fungal strains used in this study were M. furfur (KCTC 7846) and M. restricta (KCTC 7848). These strains were obtained from the Korean Collection for Type Cultures (KCTC; Daejeon, Korea).
성능/효과
) showed anti-fungal activity. Among the 29 species of Phaeophyta (brown algae) screened, only five (Dictyota dichotoma, Laminaria japonica, Myelophycus simplex. Petalonia fascia and Sargassum micracanthum') (17.2%) inhibited fungal pathogens. From the Rhodophyta (red algae) species screened, seven (Chondaria crassicaulis, Chondra canthus tenella.
0%) among the three classes of seaweeds screened. Five Chlorophyta species (Enteromorpha compressa, E. linza, Monostroma nitidum, Ulva pertusa, and Ulva sp.) showed anti-fungal activity. Among the 29 species of Phaeophyta (brown algae) screened, only five (Dictyota dichotoma, Laminaria japonica, Myelophycus simplex.
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