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Malva verticillata seed extracts upregulate the Wnt pathway in human dermal papilla cells

International journal of cosmetic science, v.38 no.2, 2016년, pp.148 - 154  

Lee, E. Y. (Department of Biotechnology, CHA University, Seongnam, Korea) ,  Choi, E.‐J. (Department of Biotechnology, CHA University, Seongnam, Korea) ,  Kim, J. A. (College of Pharmacy, Kyungpook National University, Daegu, Korea) ,  Hwang, Y. L. (OBM Laboratory, Daejeon, Korea) ,  Kim, C.‐D. (Department of Dermatology, School of Medicine, Chungnam National University, Daejeon, Korea) ,  Lee, M. H. (OBM Laboratory, Daejeon, Korea) ,  Roh, S. S. (OBM Laboratory, Daejeon, Korea) ,  Kim, Y. H. (College of Pharmacy, Chungnam National University, Daejeon, Korea) ,  Han, I. (Department of Neurosurgery, CHA University, CHA Bundang Medical Center, Seongnam, Korea) ,  Kang, S. (Department of Biotechnology, CHA University, Seongnam, Korea)

Abstract AI-Helper 아이콘AI-Helper

SynopsisObjectiveMesenchymal–epithelial interactions are important in controlling hair growth and the hair cycle. The β‐catenin pathway of dermal papilla cells (DPCs) plays a pivotal role in morphogenesis and normal regeneration of hair follicles. Deletion of β‐catenin in...

Abstract

RésuméObjectifLes interactions mésenchymateuses‐ épithéliales sont importantes dans le contrôle de la croissance des cheveux et du cycle pilaire. La voie β‐caténine des cellules des papilles dermiques (CPD) joue un rôle essentiel dans la morphogenèse et la régénération normale des follicules pileux. La suppression de la β‐caténine dans la papille dermique réduit la prolifération des cellules souches du follicule pileux qui génèrent la tige du cheveu et induit une apparition précoce de la phase catagène. Dans cette étude, un modulateur de l'activité/β‐caténine Wnt a été étudiée dans des extraits d'herbes orientales sur CPD humains cultivés.MéthodesL'effet des graines de mauve crépue (Malva verticillata) sur les CPD humaines a été étudié par un système de dosage de l'activité du gène rapporteur Wnt/β‐caténine (TCF‐β‐caténine/gène rapporteur LEF) et l'analyse de la prolifération cellulaire. La synthèse des facteurs liés à la croissance des cheveux et au cycle a été mesurée à la fois au niveau d'ARNm et des protéines par PCR semi‐quantitative et une analyse par Western blot, respectivement.RésultatsUn extrait des graines de M. verticillata a augmenté l'activité de Wnt rapporteur d'une manière dépendante de la concentration et a également conduit à des niveaux accrus de β‐caténine dans les CPD humaines en culture. L'acide myristoléique, identifié comme étant un composé efficace des graines de M. verticillata, a stimulé la prolifération des CPD d'une manière dépendante de la dose ainsi que l'augmentation des niveaux des cibles en aval de l'IGF‐1, KGF, VEGF, HGF et leur transcription. L'acide myristoleïque a également amélioré la phosphorylation des MAPK (Akt et p38).ConclusionGlobalement, les données suggèrent que cet extrait des semences de M. verticillata pourrait être un bon candidat pour le traitement de la perte de cheveux en modulant la voie/β‐caténine dans les CPD.

주제어

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