[국내논문]개량제 장기 연용이 논토양의 미생물체량, 효소활성 및 세균 다양성에 미치는 영향 Impact of Amendments on Microbial Biomass, Enzyme Activity and Bacterial Diversity of Soils in Long-term Rice Field Experiment원문보기
토양미생물 군락에 대한 유기물 및 비료의 장기 연용 효과를 평가하고자 동일비료연용 논토양을 대상으로 미생물상, 미생물체량 및 세균 군락의 상호관계에 대해 조사하였다. 유기태 탄소함량은 비료를 연용한 곳 보다 볏짚 퇴비를 시용한 처리구에서 높았다. 토양 세균수는 무비구와 화학비료 시용구에 비해 퇴비, 석회, 규산을 병용한 처리구에서 비교적 높았다. 영년동일 논토양에서 Dactylosporangium, Ewingella, Geobacillus, Kocuria, Kurthia, Kytococcus, Lechevalieria, Micrococcus, Micromonospora, Paenibacillus, Pedobacter, Pseudomonas, Pseudoxanthomonas, Rhodococcus, Rothia, Sphingopyxis, Stenotrophomonas, Variovorax 등의 세균이 분리되었으며, Arthrobacter, Kocuria, Kurhtia 및 Bacillus 등의 세균이 우점하였다. 미생물체량 및 탈수소효소 활성은 볏짚퇴비 시용구에서 가장 높았다.
토양미생물 군락에 대한 유기물 및 비료의 장기 연용 효과를 평가하고자 동일비료연용 논토양을 대상으로 미생물상, 미생물체량 및 세균 군락의 상호관계에 대해 조사하였다. 유기태 탄소함량은 비료를 연용한 곳 보다 볏짚 퇴비를 시용한 처리구에서 높았다. 토양 세균수는 무비구와 화학비료 시용구에 비해 퇴비, 석회, 규산을 병용한 처리구에서 비교적 높았다. 영년동일 논토양에서 Dactylosporangium, Ewingella, Geobacillus, Kocuria, Kurthia, Kytococcus, Lechevalieria, Micrococcus, Micromonospora, Paenibacillus, Pedobacter, Pseudomonas, Pseudoxanthomonas, Rhodococcus, Rothia, Sphingopyxis, Stenotrophomonas, Variovorax 등의 세균이 분리되었으며, Arthrobacter, Kocuria, Kurhtia 및 Bacillus 등의 세균이 우점하였다. 미생물체량 및 탈수소효소 활성은 볏짚퇴비 시용구에서 가장 높았다.
The long-term effects of soil management history on microbial communities are still poorly understood. Our objectives were to determine the impact of long-term application of soil amendments on microbial communities in rice paddy fields. The treatments selected were control where crops were grown wi...
The long-term effects of soil management history on microbial communities are still poorly understood. Our objectives were to determine the impact of long-term application of soil amendments on microbial communities in rice paddy fields. The treatments selected were control where crops were grown without any nutrient application (CON); nitrogen-phosphorus-potassium (NPK); NPK plus compost (CNPK); NPK plus lime (LNPK); and NPK plus silicate (WNPK). The long-term addition of organic and inorganic amendments significantly changed soil chemical properties. The amount of organic carbon increased in the treatments with fertilizer and amendments over that in the soil without inputs. However, we could not observe the differences of bacterial population among the treatments, but the number of aerobic bacteria increased by the addition of amendments. Isolates from the rice paddy soils before irrigation were Dactylosporangium, Ewingella, Geobacillus, Kocuria, Kurthia, Kytococcus, Lechevalieria, Micrococcus, Micromonospora, Paenibacillus, Pedobacter, Pseudomonas, Pseudoxanthomonas, Rhodococcus, Rothia, Sphingopyxis, Stenotrophomonas, and Variovorax. Dominant genera were Arthrobacter, Kocuria, Kurthia, and Bacillus in the long-term field. Microbial biomass was the highest in the compost treatment (CNPK), and was the lowest in the CON. Dehydrogenase activity in soils treated with rice compost straw was the highest and the activity showed an increasing trend according to treatment as follows: CON < WNPK < NPK = LNPK < CNPK. These results demonstrate that soil management practice, such as optimal application of fertilizer and amendment, that result in accumulations of organic carbon may increase microbial biomass and dehydrogenase activity in long-term rice paddy soils.
The long-term effects of soil management history on microbial communities are still poorly understood. Our objectives were to determine the impact of long-term application of soil amendments on microbial communities in rice paddy fields. The treatments selected were control where crops were grown without any nutrient application (CON); nitrogen-phosphorus-potassium (NPK); NPK plus compost (CNPK); NPK plus lime (LNPK); and NPK plus silicate (WNPK). The long-term addition of organic and inorganic amendments significantly changed soil chemical properties. The amount of organic carbon increased in the treatments with fertilizer and amendments over that in the soil without inputs. However, we could not observe the differences of bacterial population among the treatments, but the number of aerobic bacteria increased by the addition of amendments. Isolates from the rice paddy soils before irrigation were Dactylosporangium, Ewingella, Geobacillus, Kocuria, Kurthia, Kytococcus, Lechevalieria, Micrococcus, Micromonospora, Paenibacillus, Pedobacter, Pseudomonas, Pseudoxanthomonas, Rhodococcus, Rothia, Sphingopyxis, Stenotrophomonas, and Variovorax. Dominant genera were Arthrobacter, Kocuria, Kurthia, and Bacillus in the long-term field. Microbial biomass was the highest in the compost treatment (CNPK), and was the lowest in the CON. Dehydrogenase activity in soils treated with rice compost straw was the highest and the activity showed an increasing trend according to treatment as follows: CON < WNPK < NPK = LNPK < CNPK. These results demonstrate that soil management practice, such as optimal application of fertilizer and amendment, that result in accumulations of organic carbon may increase microbial biomass and dehydrogenase activity in long-term rice paddy soils.
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문제 정의
The application of amendment reflected different profiles in bacterial, AM fungi, and actinomycetal PLFAs in this study. The concentration of total bacterial PLFAs was the highest after organic matter amendment and low in mineral treatments, therefore these results indicated that the long-term fertilization treatments changed the microbial community structure in the soil with following report of Marschner et al.
Values of dehydrogenase in soils treated with rice compost straw was the highest in this long-term experiment in this study. The activity showed an increasing trend according to treatment as follows: CON < WNPK < NPK = LNPK < CNPK.
제안 방법
The main objectives were (1) to evaluate the effects of amendments in long-term paddy field on microbial diversity, microbial biomass, and enzymatic activities; (2) to test whether a correlation exists among microbial biomass size and enzyme activity and (3) to compare bacterial diversity by the bacterial species.
The treatments selected were control where crops were raised without any nutrient application(CON); nitrogen, phosphorus and potassium (NPK); NPK plus compost (CNPK); NPK plus lime (LNPK); and NPK plus silicate (WNPK).
Phospholipid fatty acids in soils Plot of LNPK was followed the CNPK treatment in the microbial biomass C content in this study. This pattern confirms that liming improve and sustain favorable conditions for microbial growth and activity like as application of rice straw compost.
대상 데이터
For this study, the more than 50-year old static fertilization experimental site in Korea was chosen. The main objectives were (1) to evaluate the effects of amendments in long-term paddy field on microbial diversity, microbial biomass, and enzymatic activities; (2) to test whether a correlation exists among microbial biomass size and enzyme activity and (3) to compare bacterial diversity by the bacterial species.
Soil sampling and processing The soil was collected from a rice paddy field managed for 54 years located in Suwon, Korea (37° 16'28"N/126° 59'31”E). The soil was a moderately well-drained and rapid permeability (coarse-loamy, mixed, nonacid, mesic family of Fluvaquentic Eutrudepts).
데이터처리
Statistical analysis ANOVA (one- and two-way with replicates) was used to determine significant differences among parameters. the effects of amendments and culture were evaluated by multiple comparisons among class means, based on values of a least significance (LSD) test and on Duncan's multiple range test (P<0.
parameters. the effects of amendments and culture were evaluated by multiple comparisons among class means, based on values of a least significance (LSD) test and on Duncan's multiple range test (P<0.05). The relationship among each parameter was determined by regression and multiple linear correlation coefficients (R).
05). The relationship among each parameter was determined by regression and multiple linear correlation coefficients (R).
이론/모형
Amount of cations were determined by inductively coupled plasma spectrophotometer (ICP, GBC Integra XMP). Available phosphorus determined by Lancaster method. And other selected chemical soil properties were determined using methods proposed by NIAST.
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