가감소합향원(加減蘇合香元) 향기액이 세포활성에 미치는 영향과 항산화효과에 관한 연구 The Effect of GagamSohabhwang-won(Jiajiansuhexiang-yuan) Essential Oil on Cell Activity and Anti Oxidation원문보기
Objectives : The glial cell, located in between the blood vessel and nerve cell, takes charge of the cell support, nutrition supply, elimination of body waste, and cell action. GagamSohabhwangwon(GGSH), a chinese traditional medicinal prescription has been used orally for the treatment of seizures, ...
Objectives : The glial cell, located in between the blood vessel and nerve cell, takes charge of the cell support, nutrition supply, elimination of body waste, and cell action. GagamSohabhwangwon(GGSH), a chinese traditional medicinal prescription has been used orally for the treatment of seizures, infantile, convulsion, stroke and so forth. This paper examines the effect of the GagamSohabhwangwon(GGSH) essential oil on cell activity and anti oxidation. Methods : MTT assay methods were employed to measure the cell activity based on the amount of the GagamSohabhwangwon(GGSH) essential oil by using primarily cultivated glial cell. In addition, this paper measured a viability of the glial cell after a protein active retarder control to confirm the multiplication of the cell and examined the cell extinction by the active oxygen, an extinction shielding effect with different amount of the GagamSohabhwangwon(GGSH) essential oil to observe anti oxidation. Furthermore, this paper measured a viability of the cell and phosphorylation(phosphorylation) of the protein which affects the multiplication of the glial cell. Results : When controlling the amount of the GagamSohabhwangwon(GGSH), there was a multiplication effect of the primary glial cell, the multiplication of the cell was dependent on the density of the GagamSohabhwangwon. The multiplication power of the primary glial cell was suppressed by PKA inhibiter (H89). In compliance with the active oxygen the extinction of the primary glial cell was dependent on the density of the GagamSohabhwangwon, there was a shielding effect of the cell extinction when GagamSohabhwangwon(GGSH) was preprocessed. When inducing the multiplication of the primary glial cell, phosphorylation of the Akt, BDNF, CREB, ERK and ERM were increased. Conclusions: Based on the results, GagamSohabhwangwon essential oil will have the effect which activates the nervous system cell and protects the cell through anti oxidation.
Objectives : The glial cell, located in between the blood vessel and nerve cell, takes charge of the cell support, nutrition supply, elimination of body waste, and cell action. GagamSohabhwangwon(GGSH), a chinese traditional medicinal prescription has been used orally for the treatment of seizures, infantile, convulsion, stroke and so forth. This paper examines the effect of the GagamSohabhwangwon(GGSH) essential oil on cell activity and anti oxidation. Methods : MTT assay methods were employed to measure the cell activity based on the amount of the GagamSohabhwangwon(GGSH) essential oil by using primarily cultivated glial cell. In addition, this paper measured a viability of the glial cell after a protein active retarder control to confirm the multiplication of the cell and examined the cell extinction by the active oxygen, an extinction shielding effect with different amount of the GagamSohabhwangwon(GGSH) essential oil to observe anti oxidation. Furthermore, this paper measured a viability of the cell and phosphorylation(phosphorylation) of the protein which affects the multiplication of the glial cell. Results : When controlling the amount of the GagamSohabhwangwon(GGSH), there was a multiplication effect of the primary glial cell, the multiplication of the cell was dependent on the density of the GagamSohabhwangwon. The multiplication power of the primary glial cell was suppressed by PKA inhibiter (H89). In compliance with the active oxygen the extinction of the primary glial cell was dependent on the density of the GagamSohabhwangwon, there was a shielding effect of the cell extinction when GagamSohabhwangwon(GGSH) was preprocessed. When inducing the multiplication of the primary glial cell, phosphorylation of the Akt, BDNF, CREB, ERK and ERM were increased. Conclusions: Based on the results, GagamSohabhwangwon essential oil will have the effect which activates the nervous system cell and protects the cell through anti oxidation.
Ames BN, Cathcart R, Schwiers E, Hochstein P. Uric acid provides an antioxidant defense in humans against oxidant and radical-caused aging and cancer : a hypothesis. Proc Natl Acad Sci USA. 1981;78(11):6858-62.
Aruoma OI, Kaur H, HalliwellB. Oxygen free radicals and human diseases. J R Soc Health. 1991;111(5):172-7.
辛容?. 蘇合香元 향기 및 寶丹이 중추신경 억제 및 뇌신경수용체 활성에 미치는 영향. 동국대학교 대학원 박사학위 논문. 2000.
Koo BS, Lee SI, Ha JH, Lee DU, Inhibitoty effects of the essential oil from SuHeXiang Wan on the central nervous system after inhalation. Bio. Pharm. Bull. 2004;27(4):515-9.
천수영. 加減蘇合香元 향기액 흡입에 의한 항경련작용의 기전에 관한 연구. 동국대학교 대학원 석사학위논문. 2001.
김성수. 加滅蘇合香元 향기액의 ?痛作用 및 경구투여시의 睡眠增進效果에 관한 ?究. 동국대학교 대학원 선사학위논문. 2002.
김성욱. 加減蘇合香元 향기액이 Glioma cell과 Primaty Glial cell에 미치는 효과에 대한 실험적 연구. 동국대학교 대학원 박사학위논문. 2006.
이인. 加減蘇合香元 향기액 흡입이 베타-아밀로이드로 유발된 뇌세포 손상에 미치는 효과에 관한 實驗的 ?究. 동국대학교 대학원 박사학위논문. 2007.
Liu B, Wang K, Gao HM, Mandavilli B, Wang JY, Hong JS. Molecular consequences of activated microglia in the brain: overactivation induces apoptosis. J Neurochem. 2001;77(1):182-9.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.