[논문]In vitro Effects of L-Ascorbic Acid and Acrylamide on Lymphocyte Proliferation in Young and Aged Mice

Kang, Nam-Sung; Pyo, Suhk-Neung; Sohn, Eun-Hwa

doi:10.3746/jfn.2010.15.1.019

In vitro Effects of L-Ascorbic Acid and Acrylamide on Lymphocyte Proliferation in Young and Aged Mice 원문보기

Journal of food science and nutrition, v.15 no.1, 2010년, pp.19 - 23

Kang, Nam-Sung (College of Pharmacy, Sungkyunkwan University) , Pyo, Suhk-Neung (College of Pharmacy, Sungkyunkwan University) , Sohn, Eun-Hwa (Department of Herbal Medicine Resource, Kangwon National University)

Abstract ▼ AI-Helper

This study examined the effects of Acrylamide (ACR) and L-ascorbic acid (AsA) on the proliferation of splenocytes and the mitogen-stimulated lymphocyte proliferation in young (8 weeks) and aged (82 weeks) C57BL/6male mice in vitro. AsA increased splenocyte proliferation in both groups; however, this effect was higher in old mice, while the proliferation of lymphocyte was decreased except for treatment at $1\;{\mu}g/mL$ low concentration in both mice. In addition, ACR treatment resulted in decreased LPS-induced B lymphocyte proliferation and ConA-induced T lymphocyte proliferation in both groups. However, AsA increased LPS/ConA-induced lymphocyte proliferation in young groups and had no effects in old mice except at $0.5\;{\mu}g/mL$ Thus, the present data indicate that there is no difference effect of ACR and AsA on lymphocyte proliferation, whereas the effect of AsA on mitogen-induced cell proliferation was reduced in old mice. Overall, our results suggest that various immunomodulators have differing effects of lymphocytic proliferation on young versus aged mice.

주제어

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가설 설정

The aim of present study was to determine whether an aged animal's response to ACR and AsA is different from that of a young animal.

대상 데이터

The animals were randomly distributed into five per group. Aged (82 weeks) and young (8 weeks) C57BL/6 male mice were used in this study. The mice were provided with water and food ad libitum and quarantined under a 12 hr light: 12 hr dark photoperiod in the animal care facility of the Sungkyunkwan University, Suwon, Korea.
Aged (82 weeks) and young (8 weeks) C57BL/6 male mice were used in this study. The mice were provided with water and food ad libitum and quarantined under a 12 hr light: 12 hr dark photoperiod in the animal care facility of the Sungkyunkwan University, Suwon, Korea. Animal care was performed following the Institute for Laboratory Animal Research (ILAR) guideline.

데이터처리

The results were expressed as means±SEM and analyzed via ANOVA.

성능/효과

(15) demonstrated that although some antigen-specific antibody responses were decreased, total antibody production, frequency of antibody-producing cells and proliferative response of spleen T cells were enhanced in aged mice. Our results also showed that the proliferative response of non-stimulated spleen cells from aged mice by AsA were augmented and that LPS-stimulated B lymphocyte proliferation is not impaired. In aged mice, the susceptibility of B and T cell and their functions in specific humoral and cellular immune responses may be different.

참고문헌 (15)

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