[논문]RAG1 유전자의 염기서열에 기초한 왜매치 Abbottina springeri (잉어목, 잉어과)의 분자계통학적 위치

김근용; 방인철

RAG1 유전자의 염기서열에 기초한 왜매치 Abbottina springeri (잉어목, 잉어과)의 분자계통학적 위치
Molecular Phylogenetic Position of Abbottina springeri (Cypriniformes: Cyprinidae) Based on Nucleotide Sequences of RAG1 Gene 원문보기

Korean journal of Ichthyology = 한국어류학회지, v.22 no.4, 2010년, pp.273 - 278

김근용 (순천향대학교 해양생명공학과) , 방인철 (순천향대학교 해양생명공학과)

초록
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왜매치 Abbottina springeri Banarescu and Nalbant의 분자계통학적 위치를 밝히기 위해 한국에 서식하는 버들매치속 2종과 모래주사속 5종의 핵 유전자인 recombination activating gene 1 (RAG1)의 염기서열을 분석하였다. RAG1 유전자 염기서열 정보에 기초한 계통수에서 배들매치 A. rivularis는 단계통군을 형성하는 왜매치, Biwia zezera 및 모래주사속 종들과 분리되었다. 이 계통 내에서 B. zezera는 왜매치와 모래주사속 5종을 구성된 단계통 그룹과 자매계통 관계를 보였다. 분자계통수 상에서 버들매치속 2종은 다계통군으로 나타났고, 이러한 결과는 골격 특징들에 근거한 이들의 계통적 관계를 밝힌 선행연구와 잘 일치하였다. 따라서 입의 피질돌기 유무와 부레의 골낭 유무와 크기 등과 같은 형태적 특징들에 근거한 버들배치속과 모래주사속의 현분류체계는 진화 역사를 잘 반영하지 못하는 것으로 여겨진다.

Abstract ▼ AI-Helper

Partial nucleotide sequences of nuclear protein-coding recombination activating gene 1 (RAG1) gene of two Abbottina and five Microphysogobio species residing in Korea were analyzed to elucidate the molecular phylogenetic position of A. springeri Banarescu and Nalbant. In RAG1 tree A. rivularis was clearly separated from the monophyletic lineage composed of A. springeri, Biwia zezera and Microphysogobio species. Within this lineage B. zezera showed sister-group relationship to the monophyletic group composed of A. springeri and five Microphysogobio species. Thus, our phylogenetic tree revealed the polyphyletic nature of two Abbottina species from Korea, which result is well congruent with the previous phyletic assumption based on osteological features. The current classification of Abbottina and Microphysogobio based on morphological criteria, such as the presence or absence of papillae on lips and size of swim bladder with or without encapsulation, does not reflect their true evolutionary history.

주제어

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제안 방법

In order to amplify the exon 3 fragment of RAG1 gene PCR was carried out in a 20-μL reaction volume using AccuPower® PCR Premix (Bioneer, Daejeon, Korea), including 50 ng of genomic DNA and 5 pmol of forward and reverse primers [RAG1-1495f3 (5′-CAGTAYCAYAAGATGTACCG-3′) and RAG1-3067r (5′-TTGTGAGCYTCCATRAACTT3′)], newly designed in this study.

대상 데이터

, Ann Arbor, MI, USA) and corrected manually. The sequences analyzed in this study were deposited in GenBank (http://www.ncbi.nlm.nih.gov/genbank/) under accession numbers HQ699725-HQ699731.
Fish specimens of two Abbottina and five Microphysogobio species were captured with a spoon net (mesh size: 4×4 mm) from rivers or streams of South Korea. The voucher specimens were deposited in the collection of the Department of Marine Biotechnology of Soonchunhyang University (SUC; Asan, South Korea). Their detailed sampling information was shown in Table 1.

데이터처리

The best-scoring ML tree of a thorough ML analysis was determined under the GTRGAMMAI model in 200 inferences. Statistical support was evaluated with 1,000 non-parametric bootstrap inferences.

이론/모형

0b10 (Swofford, 2002). NJ tree was reconstructed with the Kimura 2-parameter model. Robustness of tree topologies was evaluated by bootstrap analysis with 1,000 pseudoreplicates (Felsenstein, 1985).

성능/효과

5 (Hall, 1999) and refined manually. For reconstructing phylogenetic tree Rhodeus ocellatus kurumeus CTOL00544 (GenBank accession number: EU711142) and Acheilognathus tabira tabira CTOL01541 (EU409617) were used as outgroups. The nucleotide sequence matrix for RAG1 consisted of 1,488 bp.
yaluensis were also recovered as the monophyly in all tree-making algorithms with more or less weak statistical supports, with the consistent clustering of the latter two species. Meanwhile M. rapidus showed the unclear phylogenetic position among A. springeri and other Microphysogobio species, with the possible relationship to the clade of M. jeoni, M. koreensis and M. yaluensis, which was supported only in NJ tree by 60% bootstrap value.
longidorsalis. Their phylogenetic affiliation was relatively weakly supported by 52 and 73% bootstrap values in NJ and ML trees, respectively, and 0.91 posterior probability in BI tree. Microphysogobio jeoni, M.
zezera and Microphysogobio species with highest statistical supports. Within this lineage B. zezera showed the sisterhood relationship to the monophyletic group composed of A. springeri and five Microphysogobio species, of which phylogenetic affiliation was supported by 84 and 91% bootstrap values in NJ and ML trees, respectively, and 1.00 posterior probability in BI tree. Among them, A.

후속연구

yodoensis (Kawase and Hosoya, 2010). To reach the concrete final conclusion on intergeneric relationships among Abbottina, Biwia and Microphysogobio taxon samplings have to be extended for those species in the future study.

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