선택한 단어 수는 입니다.
최소 단어 이상 선택하여야 합니다.
최소 단어 이상 선택하여야 합니다.
선택한 단어 수는 30입니다.
최대 10 단어까지만 선택 가능합니다.
최대 10 단어까지만 선택 가능합니다.
제안 방법
- Thus, to expand the number of potential applications of the bacteriocins, methods must be developed for modifying these peptides. In this study, we combined pediocin PA-1, a strong antilisterial bacteriocin, with L. reuteri, a commercially important bacterium for probiotics, and produced a valueadded probiotic L. reuteri. This recombinant L.
- Thus, in this study, structural and immunity genes of pediocin PA-1 fused with a promoter and deduced signal sequence of an α-amylase that originated from a B. adolescentis strain [10] were inserted in the E. coli-lactobacilli shuttle vector pLR5cat [12], and the production of pediocin PA-1 from the recombinant L. reuteri KCTC 3679 strain was evaluated.
- To investigate whether the recombinant pediocin PA-1 was secreted or not from the recombinant L. reuteri KCTC 3679 strains, the bacteria were cultured for 13 h in 50 ml of MRS broth, centrifuged at 7,000 ×g for 10 min, and the supernatants were filtered with 0.2-µm membrane filters (Millipore, Billerica, MA, U.S.A.).
데이터처리
- SPSS v. 12.0 (Statistical Package for Social Science Software; SPSS Co., Chicago, IL, U.S.A.) was used to perform Duncan’s multiple range tests for determining significance of difference at p<0.05.
이론/모형
- reuteri KCTC 3679 transformants were confirmed by plasmid preparation (data not shown). The antimicrobial activities of the transformants were tested using an agar diffusion method. One µl of an overnight culture was spotted on a MRS agar plate and cultured for 10 h, which was overlaid with soft agar [0.
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