오이생육, 칼슘, 옥살산 및 무기성분 함량 및 칼슘-옥살산염 형성에 대한 칼슘처리 효과 Influence of Calcium Supply on the Growth, Calcium and Oxalate Contents, Mineral Nutrients and Ca-oxalate Crystal Formation of Cucumber원문보기
식물체 내에서 칼슘의 역할에 대하여 광범위하게 알려져 있지만, 다량원소의 흡수와 축적 및 옥살산 합성에 대한 칼슘의 영향에 대한 연구는 미비한 실정이다. 본 연구는 칼슘 결핍 또는 과잉에 따른 오이생육, 다량원소 흡수, 옥살산 합성 및 칼슘-옥살산 crystal 형성에 대하여 알아보고자 수행하였다. 칼슘의 결핍 또는 과잉조건하의 오이 생육과 다량원소의 흡수는 크게 저해되는 경향을 보였으며, 특히 마그네슘과는 정반대의 흡수패턴을 보였다. 칼슘처리의 증가는 오이 잎과 뿌리 중 옥살산 함량을 증가시켰으며, 오이 엽 중 칼슘과 옥살산과의 상관관계는 매우 높은 것으로 나타났다 (0.91, P<0.001). 칼슘-옥살산 crystal의 주요 형태는 prismatic 이었고, crystal은 칼슘 처리량이 증가함에 따라 많이 생성되었다. 또한 crystal의 주요 구성성분은 칼슘, 나트륨 및 염소로 나타났다.
식물체 내에서 칼슘의 역할에 대하여 광범위하게 알려져 있지만, 다량원소의 흡수와 축적 및 옥살산 합성에 대한 칼슘의 영향에 대한 연구는 미비한 실정이다. 본 연구는 칼슘 결핍 또는 과잉에 따른 오이생육, 다량원소 흡수, 옥살산 합성 및 칼슘-옥살산 crystal 형성에 대하여 알아보고자 수행하였다. 칼슘의 결핍 또는 과잉조건하의 오이 생육과 다량원소의 흡수는 크게 저해되는 경향을 보였으며, 특히 마그네슘과는 정반대의 흡수패턴을 보였다. 칼슘처리의 증가는 오이 잎과 뿌리 중 옥살산 함량을 증가시켰으며, 오이 엽 중 칼슘과 옥살산과의 상관관계는 매우 높은 것으로 나타났다 (0.91, P<0.001). 칼슘-옥살산 crystal의 주요 형태는 prismatic 이었고, crystal은 칼슘 처리량이 증가함에 따라 많이 생성되었다. 또한 crystal의 주요 구성성분은 칼슘, 나트륨 및 염소로 나타났다.
Although the roles of calcium in plant are widely known, little is known about on an antagonistic effect of macro elements, oxalate biosynthesis and main shape of crystal in cucumber plant organs. Seeds of cucumber (Cucumis sativus cv. Ijoeunbackdadagi) were germinated in perlite tray supplied with ...
Although the roles of calcium in plant are widely known, little is known about on an antagonistic effect of macro elements, oxalate biosynthesis and main shape of crystal in cucumber plant organs. Seeds of cucumber (Cucumis sativus cv. Ijoeunbackdadagi) were germinated in perlite tray supplied with distilled-deionized water. Seedlings were transplanted into aerated containers with a half strength of Ross nutrient solution. Ca levels treated in media were as follows; No-Ca, $Ca(NO_3)_2$ 0.25, 1.25 and 2.5 mmol $L^{-1}$, and $Ca(NO_3)_2$ 2.5 mmol $L^{-1}$ + $CaCl_210$, 25 and 50 mmol $L^{-1}$. Ca-deficient and -excessive conditions severely reduced cucumber growth, as compared to the control, and adversely affected an accumulation of macro elements (N, P, K, and Mg). Calcium favorably induced oxalate (acid-soluble) synthesis in leaves and roots of cucumber plant, but not in stem. Acid-soluble oxalate contents in leaves proportionally increased with Ca supply levels (0.91, P<0.001), however, this pattern was not observed in stem and roots. Ca-oxalate crystal formation and compositional analysis were examined using SEM-EDS technique in cucumber leaves. The main type of crystal revealed a prismatic crystal and main components were Ca, Na and Cl.
Although the roles of calcium in plant are widely known, little is known about on an antagonistic effect of macro elements, oxalate biosynthesis and main shape of crystal in cucumber plant organs. Seeds of cucumber (Cucumis sativus cv. Ijoeunbackdadagi) were germinated in perlite tray supplied with distilled-deionized water. Seedlings were transplanted into aerated containers with a half strength of Ross nutrient solution. Ca levels treated in media were as follows; No-Ca, $Ca(NO_3)_2$ 0.25, 1.25 and 2.5 mmol $L^{-1}$, and $Ca(NO_3)_2$ 2.5 mmol $L^{-1}$ + $CaCl_210$, 25 and 50 mmol $L^{-1}$. Ca-deficient and -excessive conditions severely reduced cucumber growth, as compared to the control, and adversely affected an accumulation of macro elements (N, P, K, and Mg). Calcium favorably induced oxalate (acid-soluble) synthesis in leaves and roots of cucumber plant, but not in stem. Acid-soluble oxalate contents in leaves proportionally increased with Ca supply levels (0.91, P<0.001), however, this pattern was not observed in stem and roots. Ca-oxalate crystal formation and compositional analysis were examined using SEM-EDS technique in cucumber leaves. The main type of crystal revealed a prismatic crystal and main components were Ca, Na and Cl.
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제안 방법
Dry weight determinations and chemical analysis The extraction and measurement of macro-nutrients were determined according to Walinga method (1989). Five randomly selected per treatment were divided into leaves, stem, and roots, and dried in an oven at 70℃ for 2 days to determine dry weights and elemental concentrations. Chemical analyses were carried out on dry weight basis with three repeats.
4). However, it was expected that Ca played different roles depending on plant parts considering no detection of oxalate in stem and highest levelsin roots, and it was needed to analyze insoluble oxalate to correctly interpret the relationship between Ca and oxalate.
The nutrient solution was replaced every 3 days. Plants were harvested between 13:00 and 14:00 at 7 and 14 days after treatment, immediately separated into leaves, stem and roots, and used for further analysis.
대상 데이터
Plant culture and treatments This study was conducted in a glass house at NAAS, RDA in 2009. Seeds of cucumber (Cucumis sativus cv.
데이터처리
Data analysis Statistical analysis of data was carriedout using ANOVA. To determine the significance of the difference between the means of treatments, least significant difference (LSD) was computed at the 5 % probability level (SAS 9.
4. Pearson correlation coefficient (n=21) between Ca and Mg and between Ca and acid-soluble oxalate in cucumber leaves exposed for 14 days under different Ca levels in media.
To determine the significance of the difference between the means of treatments, least significant difference (LSD) was computed at the 5 % probability level (SAS 9.1), and Pearson’s correlation coefficient analysis was performed to know the relationship between Ca and Mg, and between Ca and oxalate.
이론/모형
Acid-soluble oxalate determination Acid-soluble oxalate was analyzed according to Libert (1981) and Yu et al. (2002) methods. Fresh samples (0.
Dry weight determinations and chemical analysis The extraction and measurement of macro-nutrients were determined according to Walinga method (1989). Five randomly selected per treatment were divided into leaves, stem, and roots, and dried in an oven at 70℃ for 2 days to determine dry weights and elemental concentrations.
5 mM). The compositional analysis of isolated crystals was done by the EDS technique. All intensities from the EDS-profiles showed a chemical composition typically obtained for Ca oxalate crystal: Ca, Na and Cl.
성능/효과
In conclusion, Ca-deficient and -excessive conditions caused a reduction of plant growth and the uptake and accumulation of macro elements. An increased Ca supply stimulated oxalate (acid-soluble form) biosynthesis, and accumulated Ca-oxalate crystal, called a prismatic crystal, in leaf tissue.
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