[국내논문]콩의 삼투 저항성 검정에 있어서 Non-photochemical quenching의 적용 Application of Non-photochemical Quenching on Screening of Osmotic Tolerance in Soybean Plants원문보기
한발과 염 스트레스에 대한 콩의 저항성 검정에 있어서 Non-photochemical quenching (NPQ)을 적용하기 위하여, 저항성 콩 (신팔달콩 2호)와 대조구 콩 (태광콩)을 이용하여 제한적 관수 (50 m/pot/day) 와 염 (200 mmol NaCl) 처리를 한 후, 엽록소 형광반응의 변수, maximum efficiencies of photosystem II photochemistry ($F_v/F_m$), efficiencies of photosystem II photochemistry (${\Phi}_{PSII}$), NPQ와 $CO_2$ 동화율 ($P_N$) 을 측정하였다. 콩 두 품종의 엽 수분포텐셜은 한발처리에서 -0.2 MPa에서 -0.8 MPa로, 염처리에서는 -0.7 MPa에서 -1.7 MPa로 감소하였다. 염처리에서 엽 수분함량의 감소는 신팔달콩 2호에서 적었다. 두 품종 모두 엽의 수분포텐셜이 감소함에 따라 $F_v/F_m$은 변화가 없었으며, ${\Phi}_{PSII}$와 $P_N$는 감소하였다. NPQ의 경우, 신팔달콩 2호은 한발과 염처리에 모두에서 반응이 나타난 반면, 태광콩에서는 한발처리에서만 나타났다. 두 품종의 모든 처리에서 ${\Phi}_{PSII}$와 $P_N$간에 정의 상관 관계를 보였으나, $P_N$의 감소에 대한 ${\Phi}_{PSII}$의 감소 정도가 신팔달콩 2호에서 적었다. 또한 삼투처리에 따른 ${\Phi}_{PSII}$의 감소와 NPQ의 증가는 신팔달콩 2호에서만 나타나 ${\Phi}_{PSII}$와 NPQ 간의 부의 관계가 유지된 반면, 태광콩에서는 염처리에서 이들간의 연관성이 없었다. 따라서 본 연구는 삼투 저항성의 검정에 있어서 엽록소 형광반응의 단일변수($F_v/F_m$, ${\Phi}_{PSII}$, 및 NPQ)의 이용보다 ${\Phi}_{PSII}$와 NPQ 의 상호관계 분석이 더 유효한 것을 제시하였다.
한발과 염 스트레스에 대한 콩의 저항성 검정에 있어서 Non-photochemical quenching (NPQ)을 적용하기 위하여, 저항성 콩 (신팔달콩 2호)와 대조구 콩 (태광콩)을 이용하여 제한적 관수 (50 m/pot/day) 와 염 (200 mmol NaCl) 처리를 한 후, 엽록소 형광반응의 변수, maximum efficiencies of photosystem II photochemistry ($F_v/F_m$), efficiencies of photosystem II photochemistry (${\Phi}_{PSII}$), NPQ와 $CO_2$ 동화율 ($P_N$) 을 측정하였다. 콩 두 품종의 엽 수분포텐셜은 한발처리에서 -0.2 MPa에서 -0.8 MPa로, 염처리에서는 -0.7 MPa에서 -1.7 MPa로 감소하였다. 염처리에서 엽 수분함량의 감소는 신팔달콩 2호에서 적었다. 두 품종 모두 엽의 수분포텐셜이 감소함에 따라 $F_v/F_m$은 변화가 없었으며, ${\Phi}_{PSII}$와 $P_N$는 감소하였다. NPQ의 경우, 신팔달콩 2호은 한발과 염처리에 모두에서 반응이 나타난 반면, 태광콩에서는 한발처리에서만 나타났다. 두 품종의 모든 처리에서 ${\Phi}_{PSII}$와 $P_N$간에 정의 상관 관계를 보였으나, $P_N$의 감소에 대한 ${\Phi}_{PSII}$의 감소 정도가 신팔달콩 2호에서 적었다. 또한 삼투처리에 따른 ${\Phi}_{PSII}$의 감소와 NPQ의 증가는 신팔달콩 2호에서만 나타나 ${\Phi}_{PSII}$와 NPQ 간의 부의 관계가 유지된 반면, 태광콩에서는 염처리에서 이들간의 연관성이 없었다. 따라서 본 연구는 삼투 저항성의 검정에 있어서 엽록소 형광반응의 단일변수($F_v/F_m$, ${\Phi}_{PSII}$, 및 NPQ)의 이용보다 ${\Phi}_{PSII}$와 NPQ 의 상호관계 분석이 더 유효한 것을 제시하였다.
Non-photochemical quenching (NPQ) values for utilizing them to detect osmotic tolerance in plants were examined with two different soybean cultivars, an osmotic tolerant soybean (Shinpaldalkong 2) and a control soybean (Taekwangkong). Two different stresses were applied to the cultivars as the restr...
Non-photochemical quenching (NPQ) values for utilizing them to detect osmotic tolerance in plants were examined with two different soybean cultivars, an osmotic tolerant soybean (Shinpaldalkong 2) and a control soybean (Taekwangkong). Two different stresses were applied to the cultivars as the restricted irrigations of 200 and 50 ml water $pot^{-1}\;d^{-1}$ for 5 days for a control and a drought stress, respectively, and a sodium chloride solution of 200 mmol for 6 days for a salt stress. The intact leaves of the two cultivars after treatment were used to measure chlorophyll fluorescence parameters, maximum efficiencies of photosystem II photochemistry (Fv/Fm), efficiencies of photosystem II photochemistry (${\Phi}_{PSII}$), $CO_2$ assimilation rate ($P_N$), and NPQ. Leaf water potentials of the two cultivars decreased from - 0.2 to - 0.8MPa by a drought treatment and from - 0.7 to - 1.7MPa by a salt treatment. Leaf water content of Shinpaldalkong 2 after a salt treatment was less decreased than that of Taekwangkong. $F_v/F_m$ values of both cultivars were not changed, while ${\Phi}_{PSII}$ and $P_N$ were decreased proportionally to leaf water potential decrease. The response of NPQ was occurred in Shinpaldalkong 2 under the drought and salt stresses. With Taekwangkong cultivar, only drought stress referred NPQ response. The cultivar differences on chlorophyll fluorescence parameters were found in the relationships between ${\Phi}_{PSII}$ and $P_N$, and between NPQ and ${\Phi}_{PSII}$. Although the positive relationships between ${\Phi}_{PSII}$ and $P_N$ were established on all treatments of both cultivars, the decreasing rate of ${\Phi}_{PSII}$ to $P_N$ was smaller in Shinpaldalkong 2 than Taekwangkong. The NPQ was increased according to the decrease of ${\Phi}_{PSII}$ by osmotic treatments in Shinpaldalkong 2. The complementary relationships between NPQ and ${\Phi}_{PSII}$ were well maintained at all treatments in Shinpaldalkong 2, while these relationships were lost at a salt treatment in Taekwangkong. Taken together, the results suggest that analysis of complementary relationships between ${\Phi}_{PSII}$ and NPQ could be more valuable and applicable for determining osmotic tolerance than single analysis of each parameter such as $F_v/F_m$, ${\Phi}_{PSII}$ and NPQ.
Non-photochemical quenching (NPQ) values for utilizing them to detect osmotic tolerance in plants were examined with two different soybean cultivars, an osmotic tolerant soybean (Shinpaldalkong 2) and a control soybean (Taekwangkong). Two different stresses were applied to the cultivars as the restricted irrigations of 200 and 50 ml water $pot^{-1}\;d^{-1}$ for 5 days for a control and a drought stress, respectively, and a sodium chloride solution of 200 mmol for 6 days for a salt stress. The intact leaves of the two cultivars after treatment were used to measure chlorophyll fluorescence parameters, maximum efficiencies of photosystem II photochemistry (Fv/Fm), efficiencies of photosystem II photochemistry (${\Phi}_{PSII}$), $CO_2$ assimilation rate ($P_N$), and NPQ. Leaf water potentials of the two cultivars decreased from - 0.2 to - 0.8MPa by a drought treatment and from - 0.7 to - 1.7MPa by a salt treatment. Leaf water content of Shinpaldalkong 2 after a salt treatment was less decreased than that of Taekwangkong. $F_v/F_m$ values of both cultivars were not changed, while ${\Phi}_{PSII}$ and $P_N$ were decreased proportionally to leaf water potential decrease. The response of NPQ was occurred in Shinpaldalkong 2 under the drought and salt stresses. With Taekwangkong cultivar, only drought stress referred NPQ response. The cultivar differences on chlorophyll fluorescence parameters were found in the relationships between ${\Phi}_{PSII}$ and $P_N$, and between NPQ and ${\Phi}_{PSII}$. Although the positive relationships between ${\Phi}_{PSII}$ and $P_N$ were established on all treatments of both cultivars, the decreasing rate of ${\Phi}_{PSII}$ to $P_N$ was smaller in Shinpaldalkong 2 than Taekwangkong. The NPQ was increased according to the decrease of ${\Phi}_{PSII}$ by osmotic treatments in Shinpaldalkong 2. The complementary relationships between NPQ and ${\Phi}_{PSII}$ were well maintained at all treatments in Shinpaldalkong 2, while these relationships were lost at a salt treatment in Taekwangkong. Taken together, the results suggest that analysis of complementary relationships between ${\Phi}_{PSII}$ and NPQ could be more valuable and applicable for determining osmotic tolerance than single analysis of each parameter such as $F_v/F_m$, ${\Phi}_{PSII}$ and NPQ.
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문제 정의
In the previous experiment for screening environmental stress tolerant soybean cultivars breed in Korea, we found that Shinpaldalkong 2 is more drought tolerance than Taekwangkong on the apparent growth characteristics and the responses of chlorophyll fluorescence parameters. Therefore, the purposes of this experiment are to closely search the chlorophyll fluorescence parameter for screening tolerant cultivar and to find how different responses of photochemical and non-photochemical quenching in tolerant soybean occur under moderate osmotic stress conditions. The reasons for using two different stressors, drought and salt, as osmotic treatments were that (1) the control of moderate drought condition in pot would not easy by withholding or restriction of watering because plant wilting instantly accelerated around the wilting point, (2) drought treatment by restricted water supply could induce a mild osmotic stressful condition, and (3) salt treatments could induce the moderate osmotic stressful condition within a few days without salt specific effects (Munns 2002).
성능/효과
In conclusion, our results suggest that analysis of complementary relationship between ΦPSII and NPQ could be more valuable and applicable for screening osmotic tolerance in soybean plant under the osmotic stressful condition than single analysis of each chlorophyll fluorescence parameter, Fv/Fm, ΦPSII and NPQ.
6). Our NPQ data showed very interesting results that NPQ of Taekwangkong was increased only at control and drought treatment except salt treatment. In the case of Shinpaldalkong 2, however, the NPQ was also increased at salt treatment (Fig.
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