[논문]미세전류치료가 아주반트 유도 류마티스관절염 유발 흰쥐의 인터루킨-6 발현에 미치는 영향

이현민; 이상열; 장종성; 이명희; 강종호

미세전류치료가 아주반트 유도 류마티스관절염 유발 흰쥐의 인터루킨-6 발현에 미치는 영향
Effect of Microcurrent Therapy on Interleukin-6 Expression in Adjuvant Induced Rheumatoid Arthritis Rat Model 원문보기

대한물리의학회지 = Journal of the korean society of physical medicine, v.5 no.4, 2010년, pp.551 - 558

이현민 (호남대학교 물리치료학과) , 이상열 (김해대학 물리치료과) , 장종성 (영남이공대학 물리치료과) , 이명희 (대구보건대학 물리치료과) , 강종호 (남부대학교 물리치료학과)

초록
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연구목적 : 미세전류 자극이 아주반트로 류마티스 관절염을 유발한 실험동물의 염증반응과 통증에 미치는 효과를 알아보기 위하여 실험동물의 발허리발가락관절내 염증반응 정도를 나타내는 인터루킨-6(interleukin-6)의 발현과 핫플레이트(hot plate)를 이용한 발도피지연시를 측정하여 미세전류의 효과에 대하여 알아보고자 하는데 목적이 있다. 연구방법 : 실험동물은 무작위로 대조군(n=18)과 미세전류를 적용한 실험군(n=18)으로 구분하였고, 각 군당 6마리씩 1일군, 7일군, 14일군으로 배정하였다. 류마티스 관절염 유발후 1일, 7일, 14일에 모든 실험동물의 열통각 역치를 나타내는 발도피지연시와 발허리발가락관절내 인터루킨-6의 발현정도를 측정하였다. 각 집단 내의 기간에 따른 발도피지연시와 인터루킨-6의 면역반응성은 일원배치 분산분석을 실시하였고, 사후분석으로는 Duncan의 다중범위검정을 실시하였다. 실험군과 대조군을 비교하기 위하여 독립표본 t-test를 실시하였다. 연구결과 : 실험결과는 다음과 같다. 1) 아주반트 주사 1일후, 실험군과 대조군에서 인터루킨-6 면역반응성과 발도피지연시는 비슷한 양상을 보였다. 2) 인터루킨-6 면역반응성은 아주반트 주사 7일, 14일 후 대조군이 실험군보다 유의하게 증가되었다(p<.05). 3) 발도피지연시는 아주반트 주사 7일, 14일 후 실험군이 대조군보다 유의하게 증가되었다(p<.05). 결론 : 이상의 결과로부터, 미세전류 자극이 아주반트로 유발된 류마티스관절염 모델에서 활액 조직내 염증반응을 감소시키고 열통각역치는 증가되는 것을 알 수 있었다.

주제어

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문제 정의

To date, there are few studies regarding RA treatment with MNES. Thus, the objective of this paper is to examine the effect of MNES using a pro-inflammatory cytokine, such as interleukin-6 (IL-6), in an adjuvant induced RA rat model. Analgesia was determined using hot plate assay after induction.

제안 방법

Paw withdrawal latency of the hind foot of the affected side was measured using a hot plate (ⅡTC Model 39, Life Science Instruments, U.S.A) both in the experimental group and the control group at 1 day, 7 days, and 14 days after rheumatoid arthritis induction. After placing a rat on an unheated hot plate for 30 minutes, the rat was placed on a hot plate heated to a constant temperature (51.
The experimental animals used in this study included 36 Sprague-Dawley rats, regardless of gender, 8-10 weeks old, and reared under identical conditions, with an average weight 250-330g. The experimental animals were randomly divided into a control group (n=18) and an experimental group (n=18), each of which had three subgroups of 1-day, 7-day, and 14-day groups with six rats in each subgroup. The experimental group received microcurrent stimulus after rheumatoid arthritis induction and was bred in a standard cage (290mm×430mm×180mm).

대상 데이터

The experimental animals used in this study included 36 Sprague-Dawley rats, regardless of gender, 8-10 weeks old, and reared under identical conditions, with an average weight 250-330g. The experimental animals were randomly divided into a control group (n=18) and an experimental group (n=18), each of which had three subgroups of 1-day, 7-day, and 14-day groups with six rats in each subgroup.

데이터처리

An independent sample t-test was used for a comparison between the experimental group and the control group (p<.05).
Duncan’s multiple range test was performed for the ex-post analysis.
0 for Windows was used for the statistical analysis. One-way ANOVA was conducted to examine the paw withdrawal latency and immune reaction of IL-6, according to time, in each group. Duncan’s multiple range test was performed for the ex-post analysis.
Values with different superscripts in the same column are significant (p<0.05) by Duncan's multiple range test.

성능/효과

The control group showed a significant increase at 7 days and 14 days, indicating a persistent increase in inflammatory response. The experimental group showed a significant increase at 1 day and 7 days, while the increase between 7 days and 14 days was not statistically significant.
The results of this study imply that microcurrent treatment can contribute to the prevention of inflammatory reaction and pain relief in rheumatoid arthritis. Moreover, this therapy can delay the limiting of range of motion or deformation due to the progression of disease.

후속연구

Moreover, we were unable to verify the functional effect of microcurrent treatment using behavioral tests as we focused on inflammatory reaction and pain threshold. More research is needed to clarify optimal treatment regimes and the effectiveness of microcurrent therapy compared to conventional physical modalities.

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