음양곽 물추출물의 랫에서의 안전성을 검증하기 위하여 그룹당 5마리씩 총 20마리의 수컷 SD 랫에 각각 음양곽 물추출물을 체중당 10, 100, 1000 mg/kg을 1일 1회 4주간 경구투여 하였다. 대조군으로는 생리식염수를 동량 투여하였다. 임상증상, 체중 및 사료섭취량, 혈액, 혈액생화학 및 조직학적 검사를 실시하였다. 시험기간 중 임상증상, 체중, 사료섭취량의 이상변화를 관찰할 수 없었으며, 투약종료 다음날 부검결과 고환, 부고환의 무게가 용량의존적으로 증가하였으나, 정낭선은 감소경향을 나타내었다. 조직학적 검사결과 일부 변화가 관찰되었으나 건강한 쥐에서도 발견 되는 변화였다. 따라서 체중당 1000 mg까지의 투여는 임상적으로 안전한 것으로 사료된다.
음양곽 물추출물의 랫에서의 안전성을 검증하기 위하여 그룹당 5마리씩 총 20마리의 수컷 SD 랫에 각각 음양곽 물추출물을 체중당 10, 100, 1000 mg/kg을 1일 1회 4주간 경구투여 하였다. 대조군으로는 생리식염수를 동량 투여하였다. 임상증상, 체중 및 사료섭취량, 혈액, 혈액생화학 및 조직학적 검사를 실시하였다. 시험기간 중 임상증상, 체중, 사료섭취량의 이상변화를 관찰할 수 없었으며, 투약종료 다음날 부검결과 고환, 부고환의 무게가 용량의존적으로 증가하였으나, 정낭선은 감소경향을 나타내었다. 조직학적 검사결과 일부 변화가 관찰되었으나 건강한 쥐에서도 발견 되는 변화였다. 따라서 체중당 1000 mg까지의 투여는 임상적으로 안전한 것으로 사료된다.
To evaluate of oral toxicity of Epimedium koreanum nakai (EKN) water extract, three doses of EKN water extract (10, 100 and 1000 mg/kg/day) were administered to healthy Sprague-Dawley rats for 4 weeks. The results showed that there are no abnormal signs during the experimental period. The weights of...
To evaluate of oral toxicity of Epimedium koreanum nakai (EKN) water extract, three doses of EKN water extract (10, 100 and 1000 mg/kg/day) were administered to healthy Sprague-Dawley rats for 4 weeks. The results showed that there are no abnormal signs during the experimental period. The weights of testis and epididymis were increased dose-dependently, but seminal vesicle was decreased compared with control group. In hematology and serum chemistry, changes were observed within the normal ranges. In histological finding, slight to moderated changes were found but those were commonly or rarely encountered in the normal rats. These results suggest that no observed adverse effect level (NOAEL) of the oral application of EKN was considered to be more than 1000 mg/kg in SD rats under the conditions employed in this study.
To evaluate of oral toxicity of Epimedium koreanum nakai (EKN) water extract, three doses of EKN water extract (10, 100 and 1000 mg/kg/day) were administered to healthy Sprague-Dawley rats for 4 weeks. The results showed that there are no abnormal signs during the experimental period. The weights of testis and epididymis were increased dose-dependently, but seminal vesicle was decreased compared with control group. In hematology and serum chemistry, changes were observed within the normal ranges. In histological finding, slight to moderated changes were found but those were commonly or rarely encountered in the normal rats. These results suggest that no observed adverse effect level (NOAEL) of the oral application of EKN was considered to be more than 1000 mg/kg in SD rats under the conditions employed in this study.
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제안 방법
After 4 weeks administration, all rats were anesthetized and bled via caudal vena cava after measure of the body weight, and the blood samples were subjected to hematological analysis by automatic analyzer (MS9-5, Kubota, Japan). The rats were sacrificed by cervical dislocation and organs, such as heart, lung, spleen, liver, thymus, stomach, left kidney, bladder, left testis, left epididymis, prostate and lefts seminal vesicle were dissected and weighed after washing with phosphate buffered saline (PBS) and wiped out PBS from organs.
In the present study, we investigated the subacute toxicity of the EKN water extract in SD rats. The animals were received repeated doses of EKN water extract at 10, 100 and 1000 mg/kg/day for 4 weeks consecutive days. During the experimental period, no mortality and signs of toxicity were observed in the EKN water extract dosing groups, as compared with the control group.
As regards with EKN, there are few reports concerning its toxicological aspects. The purpose of this study, therefore, is to know the possible toxicity of the EKN water extract by orally 4 weeks consecutive administration.
The rats were divided into three experimental groups and one control group (n=5). The rats of experimental groups (EKN-10, -100, -1000) were administered with 10, 100 and 1,000 mg/kg/day of the EKN water extract, per os, using gastric gavage daily for 4 weeks, respectively. Control group was given with saline in the same manner.
a ; each of 10, 100 and 1,000 mg/kg/day of EKN water extract was administered each 5 rats orally for 28 days. b ; The values were expressed with means ± SD.
대상 데이터
Twenty, 3-months-old male SD rats (355 ± 24 g BW) were purchased from Orient Bio inc. (Seoul) and acclimated for 1 week.
데이터처리
All data were expressed with means ± standard deviation. The values of body weight, organ weight, CBC count, serum chemistry, DSP and sperm count were analyzed using student t test. The values significantly different with those of control group were indicated at the levels of P < 0.
성능/효과
After administration of EKN water extract for 4 weeks, the body weights of EKN-10, -100 and -1000 were increased 0%, 3.6%, -10.7% compared with control group, respectively. But there are no significant changes in the body weight.
Consequently, base on the results in the present study, it was concluded that oral administration of EKN water extract in the concentration up to 1000 mg/kg/day for 4 weeks had shown no toxicity in rats. Also, it was uncertain but recognized that the EKN revealed accelerating effects on the male reproductive organs, meanwhile it had no adverse effects on the values of blood cell count and biochemistry in SD rats.
Especially, the absolute weights of testis in EKN-100 and EKN-1000 group were increased significantly (P < 0.05), and the absolute weights of epididymis in EKN100 group were also increased significantly (P < 0.05).
On the other hand, the mean corpuscular hemoglobin concentration in EKN-10 group were increased significantly (P < 0.01), while in EKN1000 group was decreased significantly (P < 0.01) and the mean corpuscular volume in EKN-1000 group was increased significantly (P < 0.01), while that in EKN-10 and EKN-100 groups were decreased significantly with P < 0.01 and P < 0.05, respectively.
The other hands, in reproductive organ, weight of the seminal vesicle were decreased in all EKN-groups, the weight of reproductive organs such as testis and epididymis in all EKN groups were increased dose-dependently, and were significantly (P < 0.05) increased in testis of EKN-100 and -1000 group, and epididymis in EKN-100 compared with control group.
The weights and DSP of testis were increased dose-depen-dently in all EKN groups, although these values in EKN-10 and EKN-100 groups were lower than those in control group. The sperm counts of EKN-10 and EKN-1000 groups were also increased in cauda epididymis (Table 5).
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