[논문]한국 내 국부적으로 분포하는 지리사초의 알로자임 변이와 집단구조

허만규; 최주수

doi:10.5352/jls.2010.20.8.1151

한국 내 국부적으로 분포하는 지리사초의 알로자임 변이와 집단구조
Allozyme Variation and Population Structure of Carex okamotoi (Cyperaceae), a Korean Endemic Species 원문보기

생명과학회지 = Journal of life science, v.20 no.8 = no.124, 2010년, pp.1151 - 1158

초록
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한국 내 국부적으로 분포하는 지리사초(Carex okamotoi)의 14집단에 대해 유전적 다양성과 집단구조를 실시하였다. 이 식물은 세 산맥(태백산맥, 소백산맥 노령산맥)의 고지대(700~1,500 m)에 제한적으로 자생한다. 다형성을 나타내는 대립유전자좌위는 40.0%였다. 종 수준과 집단 수준에서 유전적 다양성은 낮았으며($H_{ES}$=0.106; $H_{EP}$=0.094), 집단간 분화도 낮았다($G_{ST}$=0.082). 14집단에 대한 임의 교배로부터 산출한 편차는 0.238이였다. 간접적으로 산출한 세대간 이주하는 개체들의 수는 2.78이였다(Nm=2.78). 고정지수 분석에서 이형접합체의 실질적 결핍이 일부 집단과 대립유전자좌위에서 나타났다. 집단간 유전적 동질성은 평균 0.986이였다.

Abstract ▼ AI-Helper

The genetic diversity and population structures of fourteen Carex okamotoi (Cyperaceae) populations in Korea were determined using genetic variation at 25 allozyme loci. The Carex okamotoi species is native to Korea. It is endemic to three mountains (Mt. Taeback, Mt. Sobak, and Mt. Noreong) where it is found at 700~1,500 m above sea level. The percentage of polymorphic loci was 40.0%. Genetic diversity at the species level and at the population level was low ($H_{ES}$=0.106; $H_{EP}$=0.094), and the extent of the population divergence was relatively low ($G_{ST}$=0.082). Measurement of deviation from random mating ($F_{IS}$) within the 14 populations was 0.238. An indirect estimate of the number of migrants per generation was 2.78 (Nm=2.78). Analysis of fixation indices revealed a substantial heterozygosity deficiency in some populations and at some loci. Mean genetic identity between populations was 0.986.

주제어

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문제 정의

Recently, the species has become economically important as a protectant of washout. The objectives of this study are to estimate how much allozyme diversity is maintained in the species and to describe how genetic variation is distributed within and among populations of C. okamotoi.

대상 데이터

2). Four major clusters of C. okamotoi regions, corresponding to groups of the western, southern, central, and eastern regions, revealed by the tree. Within the four major clades, associated between distance and genetic divergence were somewhat more tenuous, the two spatially closest pairs of regions (central and eastern regions) exhibited the highest genetic identities.

이론/모형

A phenetic relationship was constructed by the neighbor-joining (NJ) method [22] using the NEIGHBOR program in PHYLIP version 3.57 [4].
The genetic structure within and among populations was also evaluated using Wright's F-statistics: FIT, FIS, and FST.

성능/효과

okamotoi regions is notable. Analyses of subpopulation structure within four of the regions indicated that, on an average, 2.2% of the total genetic diversity for each region was found between subpopulations (Table 4).
238) indicates that there was a significant deficit of heterozygotes in the populations. Analysis of fixation indices, calculated for all polymorphic loci in each population, showed a slight deficiency of heterozygotes relative to Hardy-Weinberg expectations (Table 3). For example, on an individual locus level, 92.
Karron [14] reported that widespread species had a greater number of polymorphic loci than restricted congeners. For all 9 genera cited by Karron where number of alleles polymorphic loci was calculated, widespread species had more alleles than restricted species. The differences in genetic variability among Carex honoensis, Carex humilis var.
okamotoi are lower than that of its associates, the temperate-zone species. In addition, among three Korean Carex species for which there are allozyme data (Table 5), C. okamotoi had the lowest total genetic diversity (H_T). Rare and/or endemic species may become genetically depauperate, historically or prehistorically, owing to small population size, to strong directional selection for specialized niches, or to a combination of these factors [27].
In addition, the correlation between genetic distance and geographic distance was low (r=0.51, p<0.05), and indicated that geographically close populations tended to be genetically similar and about 74% of the variation in genetic distance was caused by unknown factors other than distance.
240). On a per locus basis, the proportion of total genetic variation due to differences among populations (G_ST) ranged from 0.007 for Pgi-2 to 0.341 for Fe-1 with a mean of 0.082, indicating that about 6.0% of the total allozyme variation was among populations. The estimate of gene flow based on G_ST was slightly high among Korean populations of C.
Ten of the 25 loci (40.0%) showed detectable polymorphism in at least one population, while the remaining fourteen loci (Acp, Fe-2, Gpi-1, Idh-1, Lap-1, Lap-2, Mdh-2, Mdh-3, Mdh-4, Me, Mnr-1, Per-3, Per-4, Pgd-2, and Pgm-2) were monomorphic in all populations (Table 1). Significant differences were found in allele frequencies between regions for 3 loci (Fe-1, Per-2, and Mdh-1).

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DOI : 10.5352/JLS.2010.20.8.1151 [무료]
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