콩 재조합자식계통을 이용한 콩 종자의 크기와 지방산 조성의 양적 형질 유전자좌 분석 Analysis of Quantitative Trait Loci (QTLs) for Seed Size and Fatty Acid Composition Using Recombinant Inbred Lines in Soybean원문보기
콩은 세계 유지작물시장에서 48%을 차지하는 중요한 작물이다. 콩 종실의 크기와 기름함량의 양적 및 질적 개선이 콩 육종에 있어서 가장 중요한 목적중의 하나이다. 이 연구의 목적은 종실의 크기와 지방산조성을 조절하는 양적 형질유전자좌를 밝히는 것이다. 큰올콩과 익산10호의 교배로부터 F2:10 세대의 재조합자식계통 115계통을 이용하였다. 협의 유전력 검정에서는 백립중이 0.72, 포화지방산(팔미트산 + 스테아릭산)이 0.60, 올레익산이 0.83과 리놀레익산이 0.77 및 리놀렌산이 0.81을 나타내었다. 백립중과 연관된 양적형질유전자좌는 염색체 1번, 3번, 8번, 9번과 16번 및 17번에 7개로 나타났다. 포화지방산은 염색체 17번과 19번에 2개의 독립된 양적 형질 유전자좌가 연관되어 있었다. 올레익산 함량에 대해서는 다섯 개의 독립적인 양적 형질 유전자좌가 염색체 7번, 11번, 14번과 16번 및 19번에서 확인하였다. 리놀레익산 함량에 대한 5개의 양적 형질 유전자좌는 염색체 2번, 11번, 14번과 16번 및 19번에 있었다. 리놀렌산 함량은 3개의 양적형질유전자좌가 염색체 8번과 10번 및 19번에 관련되어 있었다. 그리고 올레익산과, 리놀레익산 및 리놀렌산에 공통적으로 확인되는 주요 양적 형질 유전자좌는 염색체 19번 이었다.
콩은 세계 유지작물시장에서 48%을 차지하는 중요한 작물이다. 콩 종실의 크기와 기름함량의 양적 및 질적 개선이 콩 육종에 있어서 가장 중요한 목적중의 하나이다. 이 연구의 목적은 종실의 크기와 지방산조성을 조절하는 양적 형질 유전자좌를 밝히는 것이다. 큰올콩과 익산10호의 교배로부터 F2:10 세대의 재조합자식계통 115계통을 이용하였다. 협의 유전력 검정에서는 백립중이 0.72, 포화지방산(팔미트산 + 스테아릭산)이 0.60, 올레익산이 0.83과 리놀레익산이 0.77 및 리놀렌산이 0.81을 나타내었다. 백립중과 연관된 양적형질유전자좌는 염색체 1번, 3번, 8번, 9번과 16번 및 17번에 7개로 나타났다. 포화지방산은 염색체 17번과 19번에 2개의 독립된 양적 형질 유전자좌가 연관되어 있었다. 올레익산 함량에 대해서는 다섯 개의 독립적인 양적 형질 유전자좌가 염색체 7번, 11번, 14번과 16번 및 19번에서 확인하였다. 리놀레익산 함량에 대한 5개의 양적 형질 유전자좌는 염색체 2번, 11번, 14번과 16번 및 19번에 있었다. 리놀렌산 함량은 3개의 양적형질유전자좌가 염색체 8번과 10번 및 19번에 관련되어 있었다. 그리고 올레익산과, 리놀레익산 및 리놀렌산에 공통적으로 확인되는 주요 양적 형질 유전자좌는 염색체 19번 이었다.
Soybean [Glycine max(L.) Merr.] is an important crop, accounting for 48% of the world market in oil crops. Improvements in economic traits, such as quality and oil constituents, arethe most important objectives in soybean breeding. The objective of this study was to identify quantitative trait loci ...
Soybean [Glycine max(L.) Merr.] is an important crop, accounting for 48% of the world market in oil crops. Improvements in economic traits, such as quality and oil constituents, arethe most important objectives in soybean breeding. The objective of this study was to identify quantitative trait loci (QTLs) that control seed size and fatty acid contents in soybean. 115 $F_{2:10}$ recombinant inbred lines (RIL) developed from a cross of 'Keunolkong' and 'Iksan10' were used. Narrow-sense heritability estimates based on a plot mean on 100 seed weight, saturated fatty acid (palmitic acid + stearic acid), and oleic, linoleic, and linolenic acid content were 0.72, 0.60, 0.83, 0.77 and 0.81, respectively. The 100 seeds weight was related to seven QTLs located on chromosomes 1, 3, 8, 9, 16 and 17. Two independent QTLs for saturated fatty acid content were identified on chromosomes 17 and 19. Five independent QTLs for oleic acid content wereidentified on chromosomes7, 11, 14, 16 and 19. Five QTLs for linoleic acid content were located on chromosomes 2, 11, 14, 16 and 19. Three QTLs for linolenic acid content were located on chromosomes 8, 10 and 19. Oleic, linoleic, and linolenic acid had one major common QTL on chromosome 19. Thus, linoleic and linolenic acid content were identified as common QTLs.
Soybean [Glycine max(L.) Merr.] is an important crop, accounting for 48% of the world market in oil crops. Improvements in economic traits, such as quality and oil constituents, arethe most important objectives in soybean breeding. The objective of this study was to identify quantitative trait loci (QTLs) that control seed size and fatty acid contents in soybean. 115 $F_{2:10}$ recombinant inbred lines (RIL) developed from a cross of 'Keunolkong' and 'Iksan10' were used. Narrow-sense heritability estimates based on a plot mean on 100 seed weight, saturated fatty acid (palmitic acid + stearic acid), and oleic, linoleic, and linolenic acid content were 0.72, 0.60, 0.83, 0.77 and 0.81, respectively. The 100 seeds weight was related to seven QTLs located on chromosomes 1, 3, 8, 9, 16 and 17. Two independent QTLs for saturated fatty acid content were identified on chromosomes 17 and 19. Five independent QTLs for oleic acid content wereidentified on chromosomes7, 11, 14, 16 and 19. Five QTLs for linoleic acid content were located on chromosomes 2, 11, 14, 16 and 19. Three QTLs for linolenic acid content were located on chromosomes 8, 10 and 19. Oleic, linoleic, and linolenic acid had one major common QTL on chromosome 19. Thus, linoleic and linolenic acid content were identified as common QTLs.
* AI 자동 식별 결과로 적합하지 않은 문장이 있을 수 있으니, 이용에 유의하시기 바랍니다.
문제 정의
The primary objective of the present research was to improve the breeding efficiency of unsaturated fatty acid quality in soybean. This study was conducted to identify simple sequence repeats (SSR) markers associated with QTLs and marker-assisted selection (MAS) for oleic, linoleic, and linolenic acid contents in soybean.
The primary objective of the present research was to improve the breeding efficiency of unsaturated fatty acid quality in soybean. This study was conducted to identify simple sequence repeats (SSR) markers associated with QTLs and marker-assisted selection (MAS) for oleic, linoleic, and linolenic acid contents in soybean.
제안 방법
All significant markers from the SLG-Regr analysis were then combined into a multiple-linkage group regression (MLG-Regr) at p<0.05 to determine the combination of independent markers those were explaining the greatest amount of phenotypic variation in a given trait.
The PCR reaction was performed in a total volume of 10 μl containing 25 ng of template DNA, 0.15 M of each forward and reverse primers, 200 M of each dNTP, 2 mM MgCl2, 0.1% Triton X-100, 1× reaction buffer (10 mM Tris-HCl pH 8.5, 100 mM KCl) and 0.5 U of Taq DNA polymerase (BioBasic Taq Polymerase, Applied Bio Basic, Canada).
대상 데이터
[15]. A total of 199 soybean SSR markers (http: //soybase.agron.iastate.edu/ssr.htm) were used to screen for polymorphisms between mapping parents. The primer pairs showing parental polymorphisms were used for SSR genotyping in RIL progenies.
The well-characterized soybean cultivars, 'Keunolkong' and 'Iksan10' were used as mapping parents.
데이터처리
For each SSR and morphological marker, the class means for seed weight, protein and oil concentration were compared for significance (p<0.05) using an F-test from the Type Ⅲ mean squares, as obtained from the General Linear Model (GLM) procedure of SAS.
Means of traits, correlation, and analysis of variance were determined by SAS program. Narrow-sense heritability was calculated on a per-plot basis, using estimates of the variance components [7].
이론/모형
The oil contents were determined by auto-soxhlet method with Buchi B-811 extracted system. 2 g of ground samples was extracted by hexane for 2 hr, pre-heated for ten minutes, and then dried 1 hour at 105℃.
성능/효과
MLG-Regr analysis identified five QTLs on chromosome 2, 11, 14, 16 and 19 (Table 3). Five independent QTLs near the marker satt590 on chromosome 2, satt197 on chromosome 11, satt556 on chromosome 14, satt183 on chromosome 16, and sct010 on chromosome 19 were identified with accounting of 3.72, 7.82, 4.33, 5.98 and 7.55% of phenotypic variation, respectively. Moreover, their individual QTLs showed a relatively low phenotypic explanation though they accounted total phenotypic variation of 31.
In our results, marker on chromosome 19 turned out to be a major QTL for oil and unsaturated fatty acid content. If colligated the result of this study, it is believed that the seed composition material as oil and fatty acid content were mainly controlled by environmental stresses and they are seed size on genotypes. Finally, we suggest that both the identified QTLs and recently identified lines of germplasms in this report will promote breeders in accumulation favorable alleles for improvement in soybean quality.
55% of phenotypic variation, respectively. Moreover, their individual QTLs showed a relatively low phenotypic explanation though they accounted total phenotypic variation of 31.03% for linoleic acid content.
후속연구
If colligated the result of this study, it is believed that the seed composition material as oil and fatty acid content were mainly controlled by environmental stresses and they are seed size on genotypes. Finally, we suggest that both the identified QTLs and recently identified lines of germplasms in this report will promote breeders in accumulation favorable alleles for improvement in soybean quality.
참고문헌 (33)
Beuselinck, P. R., D. A. Sleper, and K. D. Bilyeu. 2006. An assessment of phenotype selection for linolenic acid using genetic markers. Crop Sci. 46, 747-750.
Brummer, E. C., G. L. Graef, J. Orf, J. R. Wilcox, and R. C. Shoemaker. 1997. Mapping QTL for seed protein and oil content eight soybean populations. Crop Sci. 37, 370-378.
Burton, J. W. 1987. Quantitative genetics: Results relevant to soybean breeding. In J. R. Wilcox (ed.) Soybeans: Improvement, production and uses, 2nd eds. Agron. Monogr. 16 ASA, CSSA, ANd SSSA, Madison, WI.
Chung, J., H. L. Babka, G. L. Graef, P. E. Staswick, D. J. Lee, P. B. Cregan, R. C. Shoemaker, and J. E. Specht. 2003. The seed protein, oil and yield QTL on soybean linkage group I. Crop Sci. 43, 1053-1067.
Glaudements, H. D, M. M. J. Timmermans, and H. Rijkse. 1998. The world of edible oils. pp. 1-5 Robobank International Marketing, Utrecht, the Netherlands.
Fehr, W. R., G. A. Welke, E. G Hammond, D. N. Duvick, and S. R. Cianzio. 1991. Inheritance of elevated palmitic acid content in soybean seed oil. Crop Sci. 31, 1522-1524.
Haldane, J. B. S. 1919. The combination of linkage values and the calculation of distances between the loci of linked factors. J. Genet. 8, 299-309.
Hammond, E. G. 1991. Organization of rapid analysis of lipids in many individual plants. Modern Methods of Plant Analysis 12, 321-329
Hammond, E. G. and W. R. Fehr. 1975. Oil quality improvement in soybeans - Glycine max (L.) Merr. Fette Seifen Anstrichmittle Verbunden Mit Der Zeitschrift sie Ernahrungsindustrie 77, 97-101.
Hoeck, J. A., W. R. Fehr, R. C. Shoemaker, G. A. Welke, S. L. Johnson, and S. R. Cianzio. 2003.Molecular marker analysis of seed size in soybean. Crop Sci. 43, 68-74.
Hu, F. B., M. J. Stampfer, J. E. Manson, E. Rimm, G. A. Colditz, B. A. Rosner, C. H. Hennekens, and W. C. Willett. 1997. Dietary fat intake and the risk of coronary heart disease in women. N. Eng. J. Med. 337, 1491-1499
Hu, F. B., M. J. Stampfer, J. E. Manson, E. Rimm, G. A. Colditz, B. A. Rosner, C. H. Hennekens, and W. C. Willett. 1997. Dietary fat intake and the risk of coronary heart disease in women. N. Eng. J. Med. 337, 1491-1499
Kim, H. K., S. T. Kang, M. G. Choung, C. S. Jung, K. W. Oh, I. Y. Baek, and B. G. Son. 2006. Simple sequence repeat markers linked to quantitative trait loci controlling seed weight, protein and oil content in soybean. J. Life Sci. 16, 949-954.
Keim, P., T. C. Olson, and R. C. Shoemaker. 1988. A rapid protocol for isolating soybean DNA. Soybean Genet. Newsl. 15, 150-154.
Lee, S. H., M. A. Bailey, M. A. R. Mian, T. E. Carter, E. R. Shipe, D. A. Ashley, W. A. Parrott, R. S. Hussey, and H. R. Boerma. 1996. RFLP loci associated with soybean seed protein and oil content across populations and locations.
Liu, K. 1997. Soybeans: Chemistry, technology and utilization. Chapman and Hall, New York.
Li, Z., R. F. Wilson, W. E. Rayford, and H. R. Boerma. 2002. Molecular mapping genes conditioning reduced palmitic acid content. Crop Sci. 42, 373-378.
Mansur, L. M., J. H. Orf, K. Chase, T. Jarvik, P. B. Cregan, and K. G. Lark. 1996. Genetic mapping of agronomic traits using recombinant inbred lines of soybean [Glycine max (L.) Merr.]. Crop Sci. 36, 1327-1336.
Orf, J. H., K. Chase, F. R. Alder, L. M. Mansur, and L. G. Lark. 1999. Genetics of soybean agronomic traits: II. Interaction between yield quantitative trait loci in soybean. Crop Sci. 39, 1652-1657.
Panthee, D. R., V. R. Pantalone, D. R. West, A. M. Saxton, and C. E. Sams. 2005. Quanttitative trait loci for seed protein and oil concentration, and seed size in soybean. Crop Sci. 45, 2015-2022.
Rakow, G. and D. I. McGegor. 1973. Opportunities and problems in modification of levels of rapeseed C18 unsaturated fatty acid. J. Am. Oil Chem. Soc. 50, 400-403.
Robertson J. A. and J. K. Tomas. 1976. Chemical and microbial changes in dehulled confectionary sunflower kernels during storage under controlled conditions. J. Milk Food Technol. 39, 18-23.
Son, Y. K., J. J. Hwang, S. L. Kim, Y. H. Ryu, D. C. Shin, and J. Y. Yoo. 1997. Effect of soybean cultivars Korean traditional deonjang (soybean paste) processing. Korea Soybean Digest 14, 27-36.
Topfer, R., N. Martini, and J. Schell. 1995. Modification of plant lipid synthesis. Science 268, 681-686.
Vles R. O. and J. J. Gottrnbos. 1989. Nutritional characteristics and food use of vegetable oils, pp. 63-86, In Robbelen, G. et al. (eds.), Oil crops of the World, Their Breeding and Utilization, McGraw-Hill, New York.
Wang, T., T. Harp, E. G. Hammond, J. S. Burris, and W. R. Fehr. 2001. Seed physiological performance of soybeans with altered saturated fatty acid contents. Seed Sci. Res. 11, 93-97.
Watanabe, S, T. Tajuddin, N. Yamanaka, M. hayashi, and K. Harada. 2004. Analysis of QTLs for reproduction development and seed quality traits in soybean using recombinant in bred lines. Breed. Sci. 54, 399-407.
Wilcox, J. R., J. F. Cavins, and N. C. Nielsen. 1984. Genetic alteration of soybean oil composition by a chemical mutagen. J. Am. Oil Chem. Soc. 61, 97-100.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.