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제안 방법
- Different extraction solvents (i.e., n-hexane, chloroform, ethanol, and methanol) and extraction times (i.e., ranging from 10 to 90 min) were investigated and the yields of the reference compounds were analyzed. As shown in Fig.
- In this study, we used an GC method to simultaneously determine two reference compounds in samples of Menthae herba.
- Linear regression analysis was performed by plotting the peak area ratio of each standard compound to that of the internal standard on the y-axis, versus the concentration of each component on the x-axis. The stock solutions of standard compounds were diluted to smallest concentration with methanol.
- , low, medium, high) spiked in sample extract. The precision was measured in five replicates of spiked samples for intra-day and five successive days for inter-day. The relative standard deviation (RSD) was measured to assess precision.
- The accuracy was evaluated by recovery test. The recovery test was conducted by adding three different concentrations of each known standard (i.e., low, medium, and high) to samples before extraction. The samples were extracted and analyzed using the method described above.
- The reproducibility of developed method was evaluated by the precisions of samples during intra- and inter-day. The intra- and inter-day variations of reference compounds were less than 1.
- To evaluate the accuracy of our method, recovery was measured by adding three different concentrations of mixed standard solutions to samples. Recovery values of the reference compounds ranged from 95.
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