The aim of the study was to investigate pig reference families, generated from Korean native pigs (KNP) that were crossed with Yorkshire (YS) breeds, which were used to evaluate genetic markers to select breeding animals with superior pork quality. A set of five candidate genes (PRKAG3, MC4R, CAST, ...
The aim of the study was to investigate pig reference families, generated from Korean native pigs (KNP) that were crossed with Yorkshire (YS) breeds, which were used to evaluate genetic markers to select breeding animals with superior pork quality. A set of five candidate genes (PRKAG3, MC4R, CAST, ESR, and PRLR ) was analyzed for association with pork quality traits. PRKAG3 (I199V) SNP genotypes were significantly associated with muscle moisture, protein, and fat contents. The MC4R D298N polymorphism was significantly associated with meat tenderness and color traits. The CAST polymorphism was significantly associated with muscle moisture and crude protein traits. These three genes have been associated with pork quality traits in other pig populations, and some of our results are consistent with earlier studies. In addition, two reproductive candidate genes (ESR and PRLR ) did not have significant associations. These results suggest that further study is warranted to investigate and develop more DNA markers associated with pork quality in our KNP-crossed pig families.
The aim of the study was to investigate pig reference families, generated from Korean native pigs (KNP) that were crossed with Yorkshire (YS) breeds, which were used to evaluate genetic markers to select breeding animals with superior pork quality. A set of five candidate genes (PRKAG3, MC4R, CAST, ESR, and PRLR ) was analyzed for association with pork quality traits. PRKAG3 (I199V) SNP genotypes were significantly associated with muscle moisture, protein, and fat contents. The MC4R D298N polymorphism was significantly associated with meat tenderness and color traits. The CAST polymorphism was significantly associated with muscle moisture and crude protein traits. These three genes have been associated with pork quality traits in other pig populations, and some of our results are consistent with earlier studies. In addition, two reproductive candidate genes (ESR and PRLR ) did not have significant associations. These results suggest that further study is warranted to investigate and develop more DNA markers associated with pork quality in our KNP-crossed pig families.
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제안 방법
Digested PCR products were analyzed on 2.5∼4% agarose gels, and each allele was scored manually.
, 2001). Therefore, in this study, genotyping and association analyses of 5 SNPs in the aforementioned genes were investigated in pig reference families generated from Korean native pig (KNP)-crossed Yorkshire (YS) breeds, with the objective to evaluate KNP x YS families as a reference population for exploring and identifying genetic markers to select breeding animals with superior pork quality and to understand genomic mechanisms underlying pork quality variation between KNP and YS.
이론/모형
A goodness-of-fit chi-square test was used to test for Hardy-Weinberg equilibrium (HWE) by comparing the observed number of subjects for each genotype with the expected number of subjects, assuming HWE; genotype distributions were tested at each polymorphic locus for departure from HWE. A GLM procedure in SAS (Version 9.
성능/효과
05) are listed in Table 4. Based on the results, the PRKAG3 (I199V) SNP genotypes were significantly associated with muscle moisture, protein, and fat contents. The PRKAG3 AA genotype animals had more lipids in the muscle, and the muscle lipid content was negatively correlated with muscle moisture content.
후속연구
Our study did not investigate the possible interactions between the candidate gene polymorphisms due to the limited sample size of the animals. Therefore, additional work is warranted with more animals and gene polymorphisms to develop a selection program using DNA marker information.
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