[논문]Calcineurin may regulate multiple endocytic processes in C. elegans

Song, Hyun-Ok; Ahnn, Joo-Hong

doi:10.5483/bmbrep.2011.44.2.96

Calcineurin may regulate multiple endocytic processes in C. elegans 원문보기

BMB reports, v.44 no.2, 2011년, pp.96 - 101

Song, Hyun-Ok (Department of Infection Biology, Zoonosis Research Center, Wonkwang University School of Medicine) , Ahnn, Joo-Hong (Department of Life Science, BK21 (Life Science for Global Warming Team), College of Natural Sciences, Hanyang University)

Abstract ▼ AI-Helper

Calcineurin is a serine/threonine protein phosphatase controlled by $Ca^{2+}$ and calmodulin that has been implicated in various signaling pathways. Previously, we reported that calcineurin regulates coelomocyte endocytosis in Caenorhabditis elegans. So far, simple and powerful in vivo approaches have been developed to study various endocytic processes in C. elegans. Using these in vivo assays, we further analyzed the endocytic phenotypes of calcineurin mutants. We observed that the calcineurin mutants were defective in apical endocytosis in the intestine as well as synaptic vesicle recycling in the nerve cord. However, we found that calcineurin mutants displayed normal receptor-mediated endocytosis in oocytes. Therefore, our results suggest that calcineurin may regulate specific sets of endocytic processes in nematode.

주제어

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문제 정의

The exact mechanism for the regulation of endocytosis in tissues is still unknown, even though many genes have been identified as being involved. Therefore, our results may help the understanding of the mechanisms of various endocytic pathways.

제안 방법

In this study, we further analyzed the function of calcineurin in various endocytic pathways such as yolk endocytosis in oocytes, apical endocytosis in the intestine, and synaptic vesicle recycling in the nerve cord using in vivo assay systems featuring C. elegans. We observed defects in each type of endocytosis in three calcineurin mutants: loss-of-function(lf) and gain-of-function(gf) mutants of calcineurin A, tax-6(p675) and tax-6(jh107), as well as the null mutant of calcineurin B, cnb-1(jh103).

대상 데이터

Worms were mounted on a 2% agarose pad in 10 mM levamisole (Sigma) and then photographed using Carl Zeiss (Axio Imager. A1).

성능/효과

ndocytosis) genes, are shared in oocytes and coelomocyte endocytosis (15, 18-20). Surprisingly, our results show that calcineurin mutants displayed normal RME in oocytes, suggesting that calcineurin may not regulate yolk endocytosis by oocytes or may have a minor role in RME.

후속연구

A defect in apical food uptake from the intestine as well as feeding defect may result in caloric restriction in calcineurin mutants, and this may result in smaller body size in calcineurin loss-of-function(lf) and null mutants. Further studies may be needed to examine this possibility.
At the cellular level, calcineurin is known to regulate synaptic vesicle recycling via dynamin, a large GTPase (26, 27). Thus, further study could reveal the genetic relationship between calcineurin and dynamin or other components in the regulation of synaptic vesicle recycling. Our study also provides evidence that calcineurin may function in various endocytic processes.

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