[논문]한국 내 토마토 재재종의 RAPD에 의한 동정과 유전적 다양성

허만규; 윤선주; 강선철

doi:10.5352/jls.2011.21.1.15

한국 내 토마토 재재종의 RAPD에 의한 동정과 유전적 다양성
Identification and Genetic Diversity of Korean Tomato Cultivars by RAPD Markers 원문보기

생명과학회지 = Journal of life science, v.21 no.1 = no.129, 2011년, pp.15 - 21

허만규 (동의대학교 분자생물학과) , 윤선주 ((주)바이오파머) , 강선철 (대구대학교 생명과학과)

초록
AI-Helper

재배종 토마토(Lycopersicum esculentum)는 중요 작물의 하나이다. 36 재배종에 대해 80개 RAPD (random amplified polymorphic DNA) 마커로 동정과 다형성을 조사하였다. 80개 마커 중 36개(45.0%)는 다형성을 나타내었다. 재배종 토마토에서 다형성의 탐지는 유익한 유전자형의 선택에 의한 분자 지도 발전 가능성을 제공할 수 있다. 분자 마커는 역시 식물 육종 지적 권리(PBRs)의 동정과 보호를 위해 사용될 수 있다. 한 예로써 OPC-13 시발체를 사용한 DNA 다형은 Junk Pink와 Ailsa Craighp 품종은 OPC-13-01 밴드가 결여되어 있다. OPA-12-03와 OPB-15-07는 TK-70 품종에 특이 마커로 다른 품종에는 없다. 본 연구의 재배종 토마토에서 DNA 다형은 일부 예외는 있지만 개화 타입과 관련이 있다. 이런 접근은 바람직한 토마토 품종 육성에 유전적 정보를 높이는데 유익할 것으로 사료된다.

Abstract ▼ AI-Helper

Cultivated tomato, Lycopersicum esculentum, is a very important crop. We selected 36 cultivars and studied them for identification and polymorphism by employing random amplified DNA (RAPD) analysis with 80 oligonucleotide primers. Of the 80 primers, 36 primers (45.0%) were polymorphic. Detection of polymorphism in cultivated tomato opens up the possibility of development of its molecular map by judicious selection of genotypes. Molecular markers can also be used for cultivar identification and protection of the plant breeder's intellectual property rights (plant breeders' rights, PBRs). As an example, DNA polymorphism using OPC-13 primer that did not produce the OPC-13-01 band was only found in Junk Pink and Ailsa Craighp cultivars. OPA-12-03 and OPB-15-07 were fragments specific to the TK-70 cultivar and were absent in other cultivars. DNA polymorphism in cultivated tomato in this study was correlated with a type of inflorescence, although some cultivars had exceptions. These approaches will be useful for developing marker-assisted selection tools for genetic enhancement of the tomato plant for desirable traits.

주제어

AI 본문요약
AI-Helper

* AI 자동 식별 결과로 적합하지 않은 문장이 있을 수 있으니, 이용에 유의하시기 바랍니다.

가설 설정

DNA bands obtained from a collection of 36 tomato cultivars amplified with OPC-11 primer. M: Molecular weight of standard.

제안 방법

All RAPD bands were scored manually and only unambiguously scored bands were used in the analyses. In addition, replicate cultivars were assayed in separate experiments to verify repeatability of results. Because RAPD bands are dominant markers, it was assumed that each band corresponded to a single character with two alleles, presence (1) and absence (0) of the band.

대상 데이터

In this study thirty-six cultivars of tomato from Korea were analyzed for RAPD (random amplified polymorphic DNA) markers. RAPD assay has been useful in determining genetic relationships among closely related species [5].

이론/모형

A phenetic relationship was constructed by the neighborjoining (NJ) method [19] using the NEIGHBOR program in PHYLIP version 3.57 [6].

성능/효과

In conclusion, the present study indicated the presence of DNA polymorphism in cultivated tomato using RAPDs. This open up a possibility for developing a molecular genetic map that will lead to the application of marker-assisted selection tools in genetic enhancement of cultivated tomato.
From a number of 80 primers, 44 primers did not give any clear-cut bands, or showed only smeared bands. The results with these primers were omitted from the analyses, and RAPD markers amplified 36 primers (45.0%) were analyzed. The number of bands generated by each primer varied from 2 (OPC-10) to 11 (OPC-06), with an average of 6.

후속연구

However, dendrograms obtained with RAPD marker systems did not indicate group of cultivars according to their morphological types. Thus, associating polymorphic DNA fragments with different traits requires further study using the selected trait-specific cultivars and their progenies.

참고문헌 (22)

Ballester, J. and M. C. de Vicente. 1998. Determination of F1 hybrid seed purity in pepper using PCR-based markers. Euphytica 103, 223-226.
Carelli, B. P., L. T. S. Gerald, F. G. Grazziotin, and S. Echeverrigaray. 2006. Genetic diversity among Brazilian cultivars and land laces of tomato Lycopersicon esculentum Mill. revealed by RAPD markers. Genet. Res. Crop. Evol. 53, 395-400.

상세보기
Ciccarese, F., M. Amenduni, D. Schiavone, and M. Cirulli. 1998. Occurrence and inheritance of resistance to powdery mildew (Oidium lycopersici) in Lycopersicon species. Plant Pathology 47, 417-419.

상세보기
Cooke, R. J., G. M. M. Bredemeifer, M. W. Ganal, R. Peeters, P. Isaac, and S. Rendell. 2003. Assessment of the uniformity of wheat and tomato varieties at DNA microsattellite loci. Euphytica 132, 331-341.
Demeke, T., R. P. Adams, and R. Chibbar. 1992. Potential taxonomic use of random amplified polymorphic DNA (RAPD): a case study in Brassica. Theor. Appl. Genet. 84, 990-994.

상세보기
Felsenstein, J. 1993. PHYLIP (Phylogeny Inference Package) Version 3.5s. Distributed by the Author. Department of Genetics, Univ. of Washington, Seattle.
Ghislain, M., D. Zhang, D. Fajardo, Z. Huaman, and R. J. Hijmans. 1999. Marker-associated sampling of the cultivated Andean potato Solanum phureja collection using RAPD markers. Genet. Res. Crop. Evol. 46, 547-555.

상세보기
Huang, C. C., Y. Y. Cui, C. R. Weng, P. Zabel, and P. Lindhout. 2000. Development of diagnostic PCR markers closely linked to the tomato powdery mildew resistance gene Ol-1 on chromosome 6 of tomato. Theor. Appl. Genet. 101, 918-924.

상세보기
Lee, W. S., B. S. Kim, and H. Y. Lee. 2002. Horticultural characteristics of valuable strains in cherry tomato. Korean J. Hort. Sci. Techol. 20, 74.
Helentjaris, T., G. King, M. Slocum, C. Siedenstrang, and S. Wegman. 1985. Restriction fragment polymorphisms as probes for plant diversity and their development as tools for applied breeding. Plant Mol. Biol. 5, 109-118.

상세보기
Ilbi, H. 2003. RAPD markers assisted varietal identification and genetic purity test in pepper, Capsicum annuum. SciHort. 97, 211-218.

상세보기
Kresovich, S., J. G. K. Williams, J. R. McFerson, E. J. Routman, and B. A. Schaal. 1992. Characterization of genetic identities and relationships of Brassica oleraceae L. via a random amplified polymorphic DNA assay. Theor. Appl. Genet. 85, 190-196.
Macko, A. and D. Grzebelus. 2008. DcMater trasposon display markers as a tool for diversity evaluation of carrot breeding materials and for hybrid seed purity testing. J. Appl. Genet. 49, 33-39.

상세보기
Martinez, S. G., L. Andreani, M. G. Gusano, F. Geuna, and J. J. Ruiz. 2006. Evaluation of amplified fragment length polymorphism and simple sequence repeats for tomato germplasm fingerprinting: utility for grouping closely related traditional cultivars. Genome 49, 648-656.

상세보기
Molnar, S. J., L. E. James, and K. J. Kasha. 2000. Inheritance and RAPD tagging of multiple genes for resistance to net blotch in barley. Genome 43, 224-231.

상세보기
Nei, M. 1973. Analysis of gene diversity in subdivided populations. Proc. Natl. Acad. Sci. U.S.A. 70I, 3321-3323.

상세보기
Paran, I., M. Horowitz, D Zamir, and S. Wolf. 1995. Random amplified polymorphic DNA markers are useful for purity determination of tomato hybrids. HortScience 30, 377.

상세보기
Rick, C. M. 1990. J. W. de Verna, and R. T. Chetelet. 1990. Experimental ingression to the cultivated tomato from related wild nightshades, pp. 19-30, In Bennett, A. B. and S. D. O'Neill (eds.), Horticultural biotechology. New York.
Saitou, N. and M. Nei. 1987. The neighbor-joining method: A new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4, 406-425.

상세보기
Subramanian, V., S. Gurtu, R. C. Nageswara, and S. H. Higam. 2000. Identification of DNA polymorphism in cultivated groundnut using random amplified polymorphic DNA (RAPD) assay. Genome 43, 646-660.
Williams, J. G. K, A. R. Kubelik, K. J. Livak, J. A. Rafalski, and S. V. Tingey. 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res. 18, 6531-6535.

상세보기
Yeh, F. C., R. C. Yang, and T. Boyle. 1999. POPGENE Version 1.31, Microsoft Windows-based Freeware for Population Genetic Analysis. University of Alberta, Alberta.

저자의 다른 논문 :

LOADING...

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증