한국의 잔디에서 분리한 Sclerotinia homoeocarpa 균의 체세포화합성군 분류 Vegetative Compatibility Grouping of Sclerotinia homoeocarpa Isolates Infecting Turfgrass in South Korea원문보기
동전마름병을 일으키는 Sclerotinia homoeocarpa는 한국에서 가장 일반적인 한지형 잔디 병원균이다. 경기도, 충청남도, 전라북도, 경상북도 등 다양한 지역에서 분리한 13개 균주로부터 유도된 nit 변이균주를 이용하여 의한 체세포화합성군(vegetative compatibility group, VCG) 평가가 이루어졌다. 돌연변이주는 질소동화작용에 근거하여 nit1, nit2, nit3, NitM 등 4가지 유형으로 분류되었다. 균주간에 돌연변이주 발생정도는 크게 차이가 있었으며 최대 15개의 변이주를 생산한 균주가 있는 반면에 전혀 형성하지 못한 균주도 존재하였다. 선택배지에서 4가지 돌연변이주 유형 중에 nit1과 NitM의 발생빈도(80%)가 높았으며, nit2와 nit3은 발생빈도가 매우 낮았다. 한 개의 큰 체세포화합성군 그룹과 4개의 작은 그룹이 18개 돌연변이주로부터 결정되었다. Nit 변이균주를 이용하여 의한 VCG 평가는 매우 효율적이었으며, 향후 한국의 Sclerotinia homoeocarpa균의 효율적 관리를 위한 생태학적 역학적인 면을 연구하기 위해서는 보다 많은 지역으로부터 분리된 균주의 평가가 필요하다.
동전마름병을 일으키는 Sclerotinia homoeocarpa는 한국에서 가장 일반적인 한지형 잔디 병원균이다. 경기도, 충청남도, 전라북도, 경상북도 등 다양한 지역에서 분리한 13개 균주로부터 유도된 nit 변이균주를 이용하여 의한 체세포화합성군(vegetative compatibility group, VCG) 평가가 이루어졌다. 돌연변이주는 질소동화작용에 근거하여 nit1, nit2, nit3, NitM 등 4가지 유형으로 분류되었다. 균주간에 돌연변이주 발생정도는 크게 차이가 있었으며 최대 15개의 변이주를 생산한 균주가 있는 반면에 전혀 형성하지 못한 균주도 존재하였다. 선택배지에서 4가지 돌연변이주 유형 중에 nit1과 NitM의 발생빈도(80%)가 높았으며, nit2와 nit3은 발생빈도가 매우 낮았다. 한 개의 큰 체세포화합성군 그룹과 4개의 작은 그룹이 18개 돌연변이주로부터 결정되었다. Nit 변이균주를 이용하여 의한 VCG 평가는 매우 효율적이었으며, 향후 한국의 Sclerotinia homoeocarpa균의 효율적 관리를 위한 생태학적 역학적인 면을 연구하기 위해서는 보다 많은 지역으로부터 분리된 균주의 평가가 필요하다.
Sclerotinia homoeocarpa, the causal agent of dollar spot, is one of the most common pathogens of cool season turfgrasses in South Korea. The vegetative compatibility group (VCG) assay was carried out using nitrate-nonutilizing (nit) mutants recovered from 13 South Korean isolates with various geogra...
Sclerotinia homoeocarpa, the causal agent of dollar spot, is one of the most common pathogens of cool season turfgrasses in South Korea. The vegetative compatibility group (VCG) assay was carried out using nitrate-nonutilizing (nit) mutants recovered from 13 South Korean isolates with various geographical origins. The mutants were divided into four phenotypic classes based on mutation loci associated with nitrogen assimilation: nit1, nit2, nit3, and NitM. The recovered number of nit mutants greatly varied among the isolates, ranging from 0 to 15 mutants. Of the mutants isolated, nit1 and nit2 mutants were most common (80%) while NitM and nit3 were relatively rare. One dominant and four minor VCGs were determined from 18 mutant isolates tested. To study population structures of Korean S. homoeocarpa isolates and increase our understanding of its ecological and epidemiological aspects for dollar spot management on turfgrass, more generated mutants should be tested with more diverse isolate collections.
Sclerotinia homoeocarpa, the causal agent of dollar spot, is one of the most common pathogens of cool season turfgrasses in South Korea. The vegetative compatibility group (VCG) assay was carried out using nitrate-nonutilizing (nit) mutants recovered from 13 South Korean isolates with various geographical origins. The mutants were divided into four phenotypic classes based on mutation loci associated with nitrogen assimilation: nit1, nit2, nit3, and NitM. The recovered number of nit mutants greatly varied among the isolates, ranging from 0 to 15 mutants. Of the mutants isolated, nit1 and nit2 mutants were most common (80%) while NitM and nit3 were relatively rare. One dominant and four minor VCGs were determined from 18 mutant isolates tested. To study population structures of Korean S. homoeocarpa isolates and increase our understanding of its ecological and epidemiological aspects for dollar spot management on turfgrass, more generated mutants should be tested with more diverse isolate collections.
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제안 방법
Agar plugs (5 mm in diameter) were taken from the edge of each nit mutant culture growing on the CDA, and transferred to three types of media: CDA, CDA amended with NaNO2 (0.05% w/v), and CDA amended with hypoxanthine (0.02% w/v).
대상 데이터
homoeocarpa were collected from different commercial golf courses in the northern and middle region of South Korea during 2009 and 2010. Thirteen out of the 22 S. homoeocarpa isolates used in this study spontaneously generated a total of 65 nit mutants on WAC. Of isolates mutant was recovered, the frequency of the mutants resistant to chlorate varied among the isolates.
성능/효과
In conclusion, the prevalence of the same genetic lineage among the many isolates of S. homoeocarpa supports that isolates originated from a clonal population could be involved in outbreaks of dollar spot epidemics in golf courses of South Korea. But, the negative complementation of four isolates indicate that the isolates could be biologically distinct from the dominant VCG.
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