[논문]고지방식이로 유발된 비만마우스에서 산마늘 잎추출물의 항비만효과

구세광; 정인권; 전우현; 김주완

고지방식이로 유발된 비만마우스에서 산마늘 잎추출물의 항비만효과
Allium victorialis Leaf Extract Prevents High Fat Diet Induced Obesity in Mice 원문보기

Journal of veterinary clinics = 한국임상수의학회지, v.28 no.3 = no.80, 2011년, pp.280 - 286

구세광 (대구한의대 한의학과) , 정인권 (대구한의대 한의학과) , 전우현 (대구한의대 한의학과) , 김주완 (주식회사 글루칸)

초록
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본 실험은 고지방식이로 유발된 비만마우스에서 산마늘 잎 메탄올 추출물의 항비만 효과를 알아보고자 체중변화, 사료섭취량변화, 렙틴과 아디포넥틴 수치변화, 난소주위 지방무게 변화 측정과 지방세포의 조직병리학적 검사를 실시하였으며, 메트포민을 체중당 250 mg 투여한 약물대조군과 비교평가 하였다. 비만유발을 위하여 고지방식이를 13주간 공급하였으며, 고지방식이 공급 7일째에 증류수(비만대조군), 메트포민(약물대조군), 산마늘 잎 메탄올 추출물을 투여하였다. 비만대조군은 확연한 비만이 유발되었으며, 산마늘 잎 메탄올 추출물(62.5, 125, 250 mg/kg) 투여군에서는 이러한 비만이 유의적으로 감소하였다. 결론적으로 산마늘 잎 메탄올 추출물은 비만유발 마우스에서 비만개선작용을 나타내었으며, 나아가 인체에서도 유의한 비만개선작용을 할 것으로 생각된다.

Abstract ▼ AI-Helper

The antiobese effects of Allium victorialis (AV) leaf methanol extract were evaluated in a high fat diet (HFD) supplied mice. The changes on the body weight, food consumption, leptin and adiponectin levels as well as the periovarian fat weights and histopathology of adipocytes were examined. The effects were compared with those of a group given 250 mg/kg of metformin. After 91 days of a continuous HFD supply, the mice were significantly showed obesity. However, the obesity induced by the HFD was inhibited by the AV extract treatment at the three different doses (62.5, 125 and 250 mg/kg) respectively. The results suggest that the AV methanol extract is beneficial for improving the diet-induced obesity in humans.

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문제 정의

However, there are no reports of the effects of AV extract on obesity. Therefore, this study evaluated the antiobese effects of an AV methanol extract in mice supplied with a HFD to test the hypothesis that an AV extract can improve diet-induced obesity in humans.

제안 방법

Female ICR mice (6-wk old upon receipt, SLC, Japan) were used in this study after allowing 7 days acclimatization. The animals were allocated four per polycarbonate cage in a temperature (20-25℃) and humidity (40-45%) controlled room.
The changes in body weight were calculated 6 days after initiating the HFD, at initial administration day, and then weekly until the end of the experiment using an automatic electronic balance (Precisa Instrument, Switzland). At the initiation of administration and at the end of the experimental period, all experimental animals were fasted overnight (water was provided; about 12hr) to decrease the effects of feeding.
The remaining eight rats were used as the intact control. The dosing of the test articles was initiated from 7 days after HFD supply, and each sample was administered orally once a day over a 12 weeks period.
The experimental groups were divided into a normal pellet diet (Superfeed Co., Korea) supplied an intact control diet, HFD (Diet Research, USA; Table 1) supplied control, 250 mg/ kg of metformin-administered group, and 62.5, 125 and 250 mg/ kg AV extract groups. Forty mice were supplied with a HFD.

대상 데이터

The mice were placed in a 12hr : 12hr light : dark cycle with a normal rodent pellet diet and water supplied ad libitum during acclimatization. Forty eight mice (8 per group) were selected for the experiments based on their body weight at 7 days after initiating the HFD supply to selected adapted mice. This study was carried out with prior approval of the Animal Ethical Committee, The University of Daegu Haany University.

데이터처리

The variance homogeneity was examined using the Levene test. If the Levene test indicated no significant deviations from variance homogeneity, the data was analyzed by one way ANOVA test followed by a least-significant differences multi-comparison test to determine the pairs of group comparisons that were significantly different. A non-parametric comparison test, KruskalWallis H test was conducted in the case of significant deviation from variance homogeneity in the Levene test.

이론/모형

Multiple comparison tests for different dose groups were conducted. The variance homogeneity was examined using the Levene test. If the Levene test indicated no significant deviations from variance homogeneity, the data was analyzed by one way ANOVA test followed by a least-significant differences multi-comparison test to determine the pairs of group comparisons that were significantly different.

성능/효과

In conclusion, a methanol extract of AV showed relatively good inhibitory effects on the HFD-induced obese. The results suggest that the administration of the AV extract can improve the diet-induced obesity in humans.
No meaningful changes on the mean daily food consumptions were detected in all administered groups tested as compared with HFD control except for metformin and AV extract 125 mg/kg administered groups, in which significant (p < 0.01 or p < 0.05) increases of mean daily food consumptions were detected as compared with HFD control, respectively (Fig 1).
The serum leptin levels were significantly lower in the metformin group and AV extract-administered groups (at all three doses) than the HDF controls; The values are expressed as the mean ± S.D. of eight mice.

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고지방식이로 유발된 비만마우스에서 산마늘 잎추출물의 항비만효과
Allium victorialis Leaf Extract Prevents High Fat Diet Induced Obesity in Mice 원문보기

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고지방식이로 유발된 비만마우스에서 산마늘 잎추출물의 항비만효과 Allium victorialis Leaf Extract Prevents High Fat Diet Induced Obesity in Mice 원문보기

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고지방식이로 유발된 비만마우스에서 산마늘 잎추출물의 항비만효과
Allium victorialis Leaf Extract Prevents High Fat Diet Induced Obesity in Mice 원문보기

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