고지방식이로 유발된 간과 신장의 손상에서 산마늘 메탄올 추출물의 효과를 알아보고자 하였다. 12주 동안 산마늘 메탄올 추출물 62.5, 125, 250 mg/kg/day를 투여한 결과 고지방식이로 유발된 혈청중의 AST, ALT, BUN, creatinine의 증가 억제와 간의 조직병리학적 변화를 감소시켜 지방간염과 신장의 손상이 현저하게 감소하였다. 또한, 항산화 효소인 superoxide dismutase, glutathione, catalase의 증가와 malondialdehyde의 감소로 항산화방어체계(antioxidant defense system)를 강화시켰다. 산마늘 메탄올 추출물 125 mg/kg 투여군은 대조군인 silymarin 100 mg/kg 투여군과 유사한 효과를 나타내었다. 결론적으로 산마늘 메탄올 추출물은 고지방식이로 유발되는 간과 신장의 손상을 완화하는 유용한 작용을 하는 것으로 나타났다.
고지방식이로 유발된 간과 신장의 손상에서 산마늘 메탄올 추출물의 효과를 알아보고자 하였다. 12주 동안 산마늘 메탄올 추출물 62.5, 125, 250 mg/kg/day를 투여한 결과 고지방식이로 유발된 혈청중의 AST, ALT, BUN, creatinine의 증가 억제와 간의 조직병리학적 변화를 감소시켜 지방간염과 신장의 손상이 현저하게 감소하였다. 또한, 항산화 효소인 superoxide dismutase, glutathione, catalase의 증가와 malondialdehyde의 감소로 항산화방어체계(antioxidant defense system)를 강화시켰다. 산마늘 메탄올 추출물 125 mg/kg 투여군은 대조군인 silymarin 100 mg/kg 투여군과 유사한 효과를 나타내었다. 결론적으로 산마늘 메탄올 추출물은 고지방식이로 유발되는 간과 신장의 손상을 완화하는 유용한 작용을 하는 것으로 나타났다.
The hepatoprotective and nephroprotective effects of Allium victorialis (AV) methanol extracts were investigated on high fat diet (HFD) supplied mice. Treatment of AV extracts (62.5, 125, 250 mg/kg) once a day for 12 weeks markedly decreased the liver steatohepatitis and kidney damages. AV extracts ...
The hepatoprotective and nephroprotective effects of Allium victorialis (AV) methanol extracts were investigated on high fat diet (HFD) supplied mice. Treatment of AV extracts (62.5, 125, 250 mg/kg) once a day for 12 weeks markedly decreased the liver steatohepatitis and kidney damages. AV extracts were inhibited the serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), blood urea nitrogen (BUN) and creatinine elevations and reduced the histopathological changes of livers induced by HFD supply. In addition, AV extracts strengthened the antioxidative defense system with an increased activity of superoxide dismutase (SOD), glutathione (GSH), catalase and reduced malondialdehyde (MDA) levels. The 125 mg/kg of AV extract showed similar favorable effects as compared with silymarin 100 mg/kg. It is suggested that AV methanol extract administration is beneficial to the improvement of the alleviation of liver and kidney damages in HFD supplement.
The hepatoprotective and nephroprotective effects of Allium victorialis (AV) methanol extracts were investigated on high fat diet (HFD) supplied mice. Treatment of AV extracts (62.5, 125, 250 mg/kg) once a day for 12 weeks markedly decreased the liver steatohepatitis and kidney damages. AV extracts were inhibited the serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), blood urea nitrogen (BUN) and creatinine elevations and reduced the histopathological changes of livers induced by HFD supply. In addition, AV extracts strengthened the antioxidative defense system with an increased activity of superoxide dismutase (SOD), glutathione (GSH), catalase and reduced malondialdehyde (MDA) levels. The 125 mg/kg of AV extract showed similar favorable effects as compared with silymarin 100 mg/kg. It is suggested that AV methanol extract administration is beneficial to the improvement of the alleviation of liver and kidney damages in HFD supplement.
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제안 방법
In present study, elevations of serum AST and ALT were detected after HFD supply with hypertrophy of hepatocytes related to steatohepatitis, fatty changes. 84 days of all three different dosages of AV extracts treatment favorably inhibit the serum AST and ALT elevations with hepatocyte hypertrophy and steatohepatitis at histopathological observation. Therefore, it is considered that AV extracts effectively prevents or treats the hepatic damages induced by abnormal diets.
This study evaluated the hepatoprotective and nephroprotective effects of AV methanol extracts on HFD supplied mice. In order to observe the effect of AV extracts, 62.5, 125 and 250 mg/kg of AV extracts were orally administered once a day for 84 days from 7 days after the HFD supply, and the efficacy was compared with 100 mg/kg of silymarin, a well established potent antioxidant (1,27).
Forty mice were supplied with HFD and the remaining eight rats were used as the intact control. The dosing of the test articles was initiated from 7 days after the HFD supply, and each sample was then administered orally once a day over a 12 weeks period.
Eight mice per groups, 48 mice were selected for further experiments base on the body weight at 7 days after initiation of HFD supply to selected adapted mice. The experimental groups were divided into a normal pellet diet (Superfeed Co., Korea) supplied intact control, HFD (Diet Research, USA; Table 1) supplied control, 100 mg/kg of silymarin-administered group, and 62.5, 125 and 250 mg/kg of AV extractsdosing groups. Forty mice were supplied with HFD and the remaining eight rats were used as the intact control.
This study evaluated the hepatoprotective and nephroprotective effects of AV methanol extracts on HFD supplied mice. In order to observe the effect of AV extracts, 62.
대상 데이터
(Korea) after confirming the morphology under microscopy. A voucher specimen of AV has been deposited at the herbarium located at the Department of Herbal Biotechnology, Daegu Haany University (SMN2008001Ku). The methanol AV extracts (yield 11.
Light : dark cycle was 12hr : 12hr and normal rodent pellet diet and water were supplied free to access during acclimatization. Eight mice per groups, 48 mice were selected for further experiments base on the body weight at 7 days after initiation of HFD supply to selected adapted mice. The experimental groups were divided into a normal pellet diet (Superfeed Co.
Female ICR mice (6-wk old upon receipt, SLC, JAPAN) were used in this study after 7 days of acclimatization. Animals were allocated four per polycarbonate cage in a temperature (20-25℃) and humidity (40-45%) controlled room.
데이터처리
Variance homogeneity was examined using the Levene test. If the Levene test indicated no significant deviations from variance homogeneity, the obtain data were analyzed by one way ANOVA test followed by least-significant differences multi-comparison test to determine which pairs of group comparison were significantly different. In case of significant deviations from variance homogeneity were observed at Levene test, a non-parametric comparison test, KruskalWallis H test was conducted.
이론/모형
GSH was determined by a spectrophotometric method using Ellman’s reagent (5).
Results are expressed as µmol GSH/g tissue. SOD was determined by the modified version from the method of Minami and Yoshikawa (21). Briefly, 15 µl of liver homogenate were mixed with 450 µl of cold deionized water, 125 µl of chloroform, and 250 µl of ethanol.
Multiple comparison tests for different dose groups were conducted. Variance homogeneity was examined using the Levene test. If the Levene test indicated no significant deviations from variance homogeneity, the obtain data were analyzed by one way ANOVA test followed by least-significant differences multi-comparison test to determine which pairs of group comparison were significantly different.
성능/효과
In the present study, HFD supplied mice were showed marked steatohepatitis (increases of serum AST and ALT levels, hypertrophy of hepatocytes and lipid depositions) with kidney damages (elevation of serum BUN and creatinine levels). In addition, decreases of antioxidant defense system, increases of liver MDA content with decreases of liver GSH, SOD and catalase contents were detected as results of 91 days of HFD supply in this study. However, these liver and kidney damages induced by HFD supply were marked inhibited by 84 days of continuous treatment of all three different dosages of AV extracts, and 125 mg/kg of AV extract showed similar favorable effects as compared with silymarin 100 mg/kg, respectively.
In conclusion, it is suggested that AV methanol extract administration is beneficial for the improvement of the alleviation of liver and kidney damages in HFD supplement.
Significant (p < 0.01 and p < 0.05) increases of serum AST, ALT, BUN and creatinine levels were detected in HFD control as compared with intact control.
후속연구
Therefore, it is believed that AV extracts have showed hepatoprotective and nephroprotective effects on HFD supplied mice resulting from the antioxidative and free radical scavenging activities, because the antioxiative effects of AV extracts are already reported (28) and AV extracts strengthened the antioxidative defense system with an increased activity of SOD, GSH and catalase and reduced MDA levels in the present study. However, it remains to be needed the further studies to elucidate the exact mechanism of favorable effects of AV extracts detected in this study.
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