Russian deer velvet antlers were divided into three parts and subjected to a extraction process using hot water at 100, 110, and $120^{\circ}C$ or an extraction with 70% ethanol. Each extract was analyzed for its biochemical components, including uronic acid, sulfated-glycosaminoglycans (...
Russian deer velvet antlers were divided into three parts and subjected to a extraction process using hot water at 100, 110, and $120^{\circ}C$ or an extraction with 70% ethanol. Each extract was analyzed for its biochemical components, including uronic acid, sulfated-glycosaminoglycans (sulfated-GAGs), and sialic acid, and the antioxidant and anti-acetylcholinesterase activities were investigated. Different levels of uronic acid and sulfated-GAGs were observed in the extracts according to the water temperature used for the extraction, and contents decreased with increasing extraction temperature. The upper layer of each extract showed high amounts of uronic acid and sulfated-GAGs, followed by the middle and base layers. Ethanol extraction was more effective for recovering uronic acid than sulfated-GAGs. Sialic acid content was the highest in the $110^{\circ}C$ extracts but was not observed in the ethanol extracts. Velvet antler extracts showed strong antioxidant activities against DPPH and hydrogen peroxide as well as strong reducing power in a dose-dependent manner. However, the antioxidant activities were different in each layer and according to the extraction method. Additionally, velvet antler extracts exhibited inhibitory activity against acetylcholinesterase, which is associated with Alzheimer's disease, in a dose-dependent manner. These results suggest that velvet antler extracts are useful as a functional food ingredient and/or a pharmaceutical.
Russian deer velvet antlers were divided into three parts and subjected to a extraction process using hot water at 100, 110, and $120^{\circ}C$ or an extraction with 70% ethanol. Each extract was analyzed for its biochemical components, including uronic acid, sulfated-glycosaminoglycans (sulfated-GAGs), and sialic acid, and the antioxidant and anti-acetylcholinesterase activities were investigated. Different levels of uronic acid and sulfated-GAGs were observed in the extracts according to the water temperature used for the extraction, and contents decreased with increasing extraction temperature. The upper layer of each extract showed high amounts of uronic acid and sulfated-GAGs, followed by the middle and base layers. Ethanol extraction was more effective for recovering uronic acid than sulfated-GAGs. Sialic acid content was the highest in the $110^{\circ}C$ extracts but was not observed in the ethanol extracts. Velvet antler extracts showed strong antioxidant activities against DPPH and hydrogen peroxide as well as strong reducing power in a dose-dependent manner. However, the antioxidant activities were different in each layer and according to the extraction method. Additionally, velvet antler extracts exhibited inhibitory activity against acetylcholinesterase, which is associated with Alzheimer's disease, in a dose-dependent manner. These results suggest that velvet antler extracts are useful as a functional food ingredient and/or a pharmaceutical.
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제안 방법
In this study, Russian deer velvet antler was divided into three parts, which were then subjected to extraction using hot water at three different temperatures and also 70% ethanol solution. The biochemical compositions of the extracts were analyzed, and their antioxidant and antiacetylcholinesterase activities were evaluated.
(Yeosu, Korea). The antler was divided into three parts (upper (RU), middle (RM), and base part (RB)), with a 60 g portion being used for each experiment (Fig. 1). The first extraction process was conducted with hot water at 100℃ for 1 h by autoclaving (MAC-601, Tokyo Rikakikai Co.
In this study, Russian deer velvet antler was divided into three parts, which were then subjected to extraction using hot water at three different temperatures and also 70% ethanol solution. The biochemical compositions of the extracts were analyzed, and their antioxidant and antiacetylcholinesterase activities were evaluated.
데이터처리
The mean values were compared using one-way ANOVA followed by Duncan’s test.
이론/모형
Acetylcholinesterase (AChE) inhibitory activities of velvet antler extracts were investigated using Ellman assay, and the results are depicted in Fig. 5. All of the extracts inhibited AChE activity in a dose-dependent manner, and the extracts at 100℃ in each part, including RU100, RM100, and RB100, showed potent anti-AChE activities.
DPPH scavenging activity was measured according to the method of Blois (1958). DPPH solution (1.
Hydrogen peroxide scavenging activity was determined according to the method of Müller (1985).
Sulfated-GAGs content was determined by the dimethylmethylene blue (DMB) dye binding method (Farndale et al., 1986). Briefly, the color reagent was prepared by dissolving 0.
성능/효과
4. Dose-dependent augmentation of reducing power was observed, and the 70% ethanol extracts (RUE, RME, and RBE) showed comparatively higher reducing power compared to those of the hot water extracts. In addition, the upper part extracts (RU100, RU110, and RU120) possessed higher reducing power than those of the middle (RM100, RM110, and RM120) and base part extracts (RB100, RB110, and RB120).
All of the extracts inhibited AChE activity in a dose-dependent manner, and the extracts at 100℃ in each part, including RU100, RM100, and RB100, showed potent anti-AChE activities. On the other hand, RUE, RME, and RBE also inhibited AChE activity, and the levels of inhibition were higher and/or similar compared to those of RU100, RM100, and RB100.
2, velvet antler extracts obtained from each part effectively quenched DPPH radical in a dose-dependent manner. The DPPH scavenging activity decreased with increasing temperature, and RU100, RM100, and RB100, which were extracted at 100℃ from each part, exhibited the highest scavenging activities. The 70% ethanol RUE, RME, and RBE also showed DPPH radical scavenging activities in a dose-dependent manner, whereas their scavenging activities were lower than those of RU100, RM100, and RB100, respectively.
The RU100, RM100, and RB100 extracts at 100℃ showed good scavenging ability compared to those of the RU110, RM110, and RB110 and RU120, RM120, and RB120 extracts.
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