[논문]Porcine Fatty Acid Synthase Gene Polymorphisms Are Associated with Meat Quality and Fatty Acid Composition

Kim, Sang-Wook; Choi, Yang-Il; Choi, Jung-Suck; Kim, Jong-Joo; Choi, Bong-Hwan; Kim, Tae-Hun; Kim, Kwan-Suk

doi:10.5851/kosfa.2011.31.3.356

Porcine Fatty Acid Synthase Gene Polymorphisms Are Associated with Meat Quality and Fatty Acid Composition 원문보기

Korean journal for food science of animal resources = 한국축산식품학회지, v.31 no.3, 2011년, pp.356 - 365

Kim, Sang-Wook (Department of Animal Science, Chungbuk National University) , Choi, Yang-Il (Department of Animal Science, Chungbuk National University) , Choi, Jung-Suck (Department of Animal Science, Chungbuk National University) , Kim, Jong-Joo (School of Biotechnology, Yeungnam University) , Choi, Bong-Hwan (Division of Animal Genomics and Bioinformatics, National Institute of Animal Science) , Kim, Tae-Hun (Division of Animal Genomics and Bioinformatics, National Institute of Animal Science) , Kim, Kwan-Suk (Department of Animal Science, Chungbuk National University)

Abstract ▼ AI-Helper

We assessed the effects of single-nucleotide polymorphisms (SNPs) within the porcine fatty acid synthase (FASN) gene regarding meat quality and fatty acid composition in two pig populations: Korean native pigs (KNP) were crossed with Yorkshire (YS) $F_2$, and KNP were crossed with Landrace (LR) $F_2$. Direct DNA sequencing using eight KNP and eight YS pigs revealed two SNPs: c.265C>T (silent) in exon 4 and c.6545A>C (Asn${\rightarrow}$His) in exon 39. The frequency of the two SNPs was analyzed using the polymerase chain reaction-restriction fragment length polymorphism method in seven pig breeds and their association with meat quality traits and fatty acid composition was studied. In the $KNP{\times}YSF_2$ population, both SNPs were significantly associated with the level of monounsaturated fatty acids, including palmitoleic (C16:1) and oleic acid (C18:1) (pC was associated with intramuscular fat content in both populations. Our results indicate that variations in c.265C>T and c.6545A>C of the pig FASN can be used to select animals with better fatty acid composition and meat quality. Moreover, KNP was a useful breed for identifying genetic factors affecting meat quality and fatty acid composition and for producing high quality pork.

주제어

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문제 정의

Therefore, the objectives of this study were to identify variations in porcine FASN, to determine if the variations are genetic factors that affect meat quality and fatty acid composition, and then to evaluate the traits of different breeds with respect to the FASN gene.

제안 방법

Primers for exons 38-41 of FASN were created in two steps. First, GenBank sequences from pig (AY954688), cattle (AF285607), human (AY451392), and goat (DQ915966) were obtained using the NCBI BLAST program (http://blast.ncbi.nlm.nih.gov/), and primers in exon regions 38-41 spanning pig FASN introns were designed using Oligo 6 software. These primer sets were then used to sequence the PCR product.
In this study, two SNPs, c.265C>T (Silent) and c.6545A>C (Asn→His), were rediscovered and characterized in several commercially important pig breeds and their association with fatty acid composition and meat quality was studied.
The association of three haplotype classes with collected economic traits was also analyzed using the same model used to analyze the effects of individual genotypes.

데이터처리

Genotypic effects of variations in the FASN gene (c.265C>T and c.6545A>C) were estimated with mixed-model analysis using the SAS 9.1 Package/PC and the differences between genotypes were examined using least significant difference tests.
is the random error. The least-square means of the genotypes estimated using these equations were tested using the t-test, and the phenotypic differences between haplotypes were tested using contrast tests.

성능/효과

05). It was apparent that animals with the CC genotype had better WHC values than animals with a CT or TT genotype. In addition, we combined the KNP × YS F₂ and KNP × LR F₂ populations to analyze five meat quality measurements in the combined population: C-pro, IMF, C-ash, WHC and 24-h pH, which have common phenotypic data between them.
In addition, we combined the KNP × YS F₂ and KNP × LR F₂ populations to analyze five meat quality measurements in the combined population: C-pro, IMF, C-ash, WHC and 24-h pH, which have common phenotypic data between them. Of the five meat quality measurements in the combined population, WHC and 24-h pH showed significant differences; individuals with the CC genotype had better WHC and higher 24-h pH than individuals with a CT or TT genotype (Table 4). Data from the fatty acid composition analysis of the KNP × YS F₂ population showed highly significant effects on C16:1 and C18:1 (p = 0.
Of the five meat quality traits in the combined population, C-pro, IMF, WHC, and 24-h pH showed a significant association (p<0.05).
The fatty acid composition analysis of the KNP × YS F2 population had highly significant effects on C16:1 and C18:1 (P =0.0008 and 0.0036, respectively); individuals with an AA or CA genotype had higher C18:1 contents than individuals with the CC genotype (Table 5).
The frequency of the A allele in the five pig breeds was examined through the analysis of the c.6545A>C (Asn→His) genotypes, and the results show that the frequency of the A allele was higher in the Korean native (1.00) and Berkshire (0.73) breeds than in the Landrace (0.50), Yorkshire (0.40), or Duroc (0.50) breeds.
This study revealed that the FASN gene is closely linked with variation of fatty acids that affect the meat quality of pigs and that the KNP breed has better meat quality and fatty acid composition than foreign breeds. These results provide an important basis for discovering genetic factors related to diverse and differential meat quality and fatty acid composition in different pig breeds.

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