Amyloid beta ($A{\beta}$)-induced neurotoxicity is a major pathological mechanism of Alzheimer's disease (AD). In this study, we investigated the inhibitory effect of L-theanine, a component of green tea (Camellia sinensis) on $A{\beta}_{1-42}$-induced neurotoxicity and oxidati...
Amyloid beta ($A{\beta}$)-induced neurotoxicity is a major pathological mechanism of Alzheimer's disease (AD). In this study, we investigated the inhibitory effect of L-theanine, a component of green tea (Camellia sinensis) on $A{\beta}_{1-42}$-induced neurotoxicity and oxidative damages of macromolecules. L-theanine inhibited $A{\beta}_{1-42}$-induced generation of reactive oxygen species, and activation of extracellular signal-regulated kinase and p38 mitogenic activated protein kinase as well as the activity of nuclear factor kappa-B. L-theanine also signifi cantly reduced oxidative protein and lipid damage, and elevated glutathione level. Consistent with the reduced neurotoxic signals, L-theanine (10-50 ${\mu}g$/ml) concomitantly attenuated $A{\beta}_{1-42}$ (5 ${\mu}M$)-induced neurotoxicity in SK-N-MC and SK-N-SH human neuroblastoma cells. These data indicate that L-theanine on $A{\beta}$-induced neurotoxicity prevented oxidative damages of neuronal cells, and may be useful in the prevention and treatment of neurodegenerative disease like AD.
Amyloid beta ($A{\beta}$)-induced neurotoxicity is a major pathological mechanism of Alzheimer's disease (AD). In this study, we investigated the inhibitory effect of L-theanine, a component of green tea (Camellia sinensis) on $A{\beta}_{1-42}$-induced neurotoxicity and oxidative damages of macromolecules. L-theanine inhibited $A{\beta}_{1-42}$-induced generation of reactive oxygen species, and activation of extracellular signal-regulated kinase and p38 mitogenic activated protein kinase as well as the activity of nuclear factor kappa-B. L-theanine also signifi cantly reduced oxidative protein and lipid damage, and elevated glutathione level. Consistent with the reduced neurotoxic signals, L-theanine (10-50 ${\mu}g$/ml) concomitantly attenuated $A{\beta}_{1-42}$ (5 ${\mu}M$)-induced neurotoxicity in SK-N-MC and SK-N-SH human neuroblastoma cells. These data indicate that L-theanine on $A{\beta}$-induced neurotoxicity prevented oxidative damages of neuronal cells, and may be useful in the prevention and treatment of neurodegenerative disease like AD.
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대상 데이터
The membrane was then incubated for 3 h at room temperature with specific antibodies. Rabbit polyclonal antibodies against active form of JNK1 (1:500), ERK2 (1:500) and p38 MAPK (1:500), p65 (1:500) mouse polyclonal antibodies against phosphorylation forms of JNK1 (1:500), ERK2 (1:500) and p38 MAPK (1:500), and p50 (1:500) (Santa Cruz Biotechnology Inc., Santa Cruz, CA) were used in this study. The blots were then incubated with the corresponding conjugated anti-rabbit, anti-mouse and anti-goat immunoglobulin G-horseradish peroxidases (Santa Cruz Biotechnology Inc.
데이터처리
Statistical analysis of the data was carried out using analysis of variance (ANOVA) for repeated measures followed by Dunnette’s post-hoc analysis using GraphPad Prism 4 software (Version 4.03, GraphPad software, Inc.).
이론/모형
Gel mobility shift assay was done using a slight modifi cation of a previously described method (Hong et al., 2000). Briefl y, the cultured cells were washed three times with icecold phosphate buffered saline (PBS, pH 7.
After centrifugation at 15,000×g for 6 min, the supernatant contained the nuclear proteins. The protein level was determined by a microplate modifi cation of the Bradford method (Bio-Rad Bulletin 1177, Bio-Rad Lab., Richmond, CA). The DNA binding activity of transcription factors was assayed according to the manufacturer’s instructions (Promega Co.
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