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제안 방법
- Accordingly, this study was designed to clarify the possibility of Rg1 as an effective candidate to ameliorate hyperglycemia and hyperlipidemia in a diabetic animal model (db/db mice).
- One day before the sacrifice, an intraperitoneal glucose tolerance test (IPGTT) was conducted on all of the db/db mice after a 12-h overnight fast. To determine the glucose tolerance, the mice were intraperitoneally injected with glucose (0.
- The blood glycated hemoglobin (HbA1c) of each sample sacrificed mice was measured using a MicroMatTM II Hemoglobin A1c Test (Bio-Rad Laboratories, Hercules, CA, USA). The plasma insulin and glucagon (Mouse ELISA Kit, ALPCO Diagnostics, Salem, NH, USA) levels were determined using a quantitative sandwich enzyme immunoassay kit.
- All groups were fed a standard AIN-76 semi-synthetic diet [2,3]. The mice had free access to food and water, and their body weight was measured once every five days throughout the experiment. The diabetic control group was orally administered deionized water and two experimental groups (acarbose, and Rg1) were orally administered acarbose (50 mg/kg BW) or Rg1 (10 mg/kg BW) for 15 days.
- The diabetic control group was orally administered deionized water and two experimental groups (acarbose, and Rg1) were orally administered acarbose (50 mg/kg BW) or Rg1 (10 mg/kg BW) for 15 days. The mice were then starved for 12 hrs, after which they were anesthetized with ether and their blood samples were taken from the inferior vena cava to measure the plasma biomarkers (HbA1c, insulin, glucagon, etc.). The mice were handled in strict accordance with the Pusan National University guidelines for the care and use of laboratory animals.
- One day before the sacrifice, an intraperitoneal glucose tolerance test (IPGTT) was conducted on all of the db/db mice after a 12-h overnight fast. To determine the glucose tolerance, the mice were intraperitoneally injected with glucose (0.5 g/kg BW) and the glucose concentrations of blood drawn from the tail vein were determined immediately upon collection at 30, 60, and 120 min after glucose injection using a Glucometer (GlucoDr, Allmedicus, Anyang, Korea).
대상 데이터
- Twenty one male C57Bl/KsJ-db/db mice (8 weeks old) were obtained from Jackson Laboratory (Bar Harbor, ME, USA). The mice were all individually housed in polycarbonate cages under a 12-h light-dark cycle at, 21-23℃ and 40-60% humidity.
데이터처리
- Significant difference between two groups was determined using Student’s t-test.
- The data were evaluated by one-way ANOVA and the differences between means were determined using Tukey’s multiple-range test.
이론/모형
- In addition, the plasma concentrations of glucose, total cholesterol, triglyceride, and HDL-cholesterol (Asan Diagnostics, Seoul, Korea) were determined using an enzymatic method. The plasma free fatty acids (FFAs) concentrations were determined using an enzymatic colorimetric method (Wako Pure Chemical Industries, Japan). All blood samples obtained were centrifuged at 1,000× g for 15 min at 4℃ for biochemical analysis.
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