[논문]흰쥐에서 흑마늘 추출물과 그 성분들에 의한 혈소판 응집억제 효과

최유희; 정형민; 경규항; 류병호; 이광열

doi:10.5352/jls.2011.21.10.1355

흰쥐에서 흑마늘 추출물과 그 성분들에 의한 혈소판 응집억제 효과
Aged Garlic Extract and Its Components Inhibit Platelet Aggregation in Rat 원문보기

생명과학회지 = Journal of life science, v.21 no.10 = no.138, 2011년, pp.1355 - 1363

최유희 (전남대학교 약학대학) , 정형민 (전남대학교 약학대학) , 경규항 (세종대학교 생명과학대학 식품공학부) , 류병호 (경남 남해군 고현면 도울농산영농조합) , 이광열 (전남대학교 약학대학)

초록
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많은 임상 실험을 통해 마늘(Allium sativum)이 심혈관 질환에 유효한 효과가 알려져 있다. 흑마늘(AGE)은 항산화, 항염증, 항암 효과 등 다양한 생물학적 효과가 보고되었다. 그러나, 흑마늘의 심혈관계 질환과의 상관관계에 대한 연구는 아직 미흡한 실정이다. 흑마늘과 그 유래 성분이 혈소판에 미치는 영향을 조사하였다. 백서의 혈액을 얻어, 흑마늘과 그 유래 성분의 thrombin에 의해 유도되는 혈소판 응집에 대한 억제 효과를 조사한 결과, 흑마늘과 diallyl sulphides는 농도의존적으로 혈소판 응집을 억제함을 관찰하였다. 또한 in vivo 실험에서도 마늘 오일과 S-Allylmercapto-cystein (SAMC)는 thrombin과 ADP에 의해 유도되는 혈소판 응집을 유의성 있게 억제하는 것을 관찰하였다. 흑마늘 유래 SAMC와 diallyl sulphides가 혈소판 응집 억제 효과를 나타내는 것을 확인할 수 있었다. 이러한 결과는 흑마늘이 심혈관계 질환의 예방을 위한 건강보조식품으로의 새로운 가능성을 제시한다.

Abstract ▼ AI-Helper

Many clinical trials have demonstrated the beneficial effects of garlic (Allium sativum) on general cardiovascular health. Aged garlic extract (AGE) is known to display diverse biological activities such as in antioxidant, anti-inflammatory and anticancer activities. However, few studies have been directed on the effect of AGE on cardiovascular function. In this study, we aimed to investigate the effect of AGE and its components on platelet activation, a key contributor in thrombotic diseases. In freshly isolated rat platelets, AGE and its components have shown inhibitory activities on thrombin-induced platelet aggregation. These in vitro results were further confirmed in an in vivo platelet aggregation measurement where tail vein injection of garlic oil and S-Allylmercapto-cysteine (SAMC) significantly reduced thrombin and ADP-induced platelet aggregation. Potential active components for antiplatelet effects of AGE were identified to be SAMC and diallyl sulphide through agonist-induced platelet aggregation assay. These results indicate that aged garlic extract can be a novel dietary supplement for the prevention of cardiovascular risks and the improvement of blood circulation.

주제어

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문제 정의

In this study, we demonstrated that AGE and its components can inhibit agoinsts-induced platelet aggregation. These in vitro results were further confirmed in an in vivo platelet aggregation where single oral administration of garlic oil reduced platelet aggregation.

제안 방법

Water soluble sulfur compound, S-allylmerceptocysteine (SMAC) showed that the inhibitory effect on platelet aggregation than followed by SAC. Also, individual components of AGE were tested for their effects on ADP or collagen induced platelet aggregation. SAMC, garlic oil and three dially sulphides inhibited ADP induced platelet aggregation in a dose-dependent manner (Fig.
For the identification of active components for antiplatelet effects of AGE, individual components of AGE (garlic oil, SAC, SAMC, DAS, DADS, DATS) were tested for their effects on thrombin induced platelet aggregation. Total percentage aggregation of platelet was reduced concentration-dependent manner (Fig.
To confirm the antiplatelet effect of SAMC and garlic oil, we conducted an in vivo platelet aggregation measurement after single or multiple oral administration of SAMC (150 μg/kg body weight once daily) and garlic oil (1 ml or 3 ml/kg body weight once daily) as control corn oil.

대상 데이터

AGE was donated by Do-Wool nongsan. Diallyl sulphide (DAS) was obtained from Fluka and diallyl disulphide (DADS) was obtained from Sigma-Aldrich Chemical (St. Louis, MO, USA). Diallyl trisulphide (DATS) was obtained from LKT.
Trisodium citrate, ethanol, ethyl acetate, methanol, dimethyl sulfoxide, adenosine, NaCl, KCl, MgCl₂, HEPES, glucose, NaHCO₃, Na₂HPO₄, CaCl₂, glutaraldehyde, EDTA, urethane, and bovine serum albumin were obtained from Sigma-Aldrich Chemical (St. Louis, MO, USA). Collagen was from Chrono-log (Harvertown, PA, USA).

데이터처리

Data were analyzed using Student’s t-test, with p values <0.05 taken to indicate statistical significance.

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