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제안 방법
- , 2011). In this study, in consideration of economic aspects, the culture broth of the by-product of A. tamarii isolated from dead silkworms was used. A variety of physiological effects of A.
대상 데이터
- Louis, MO, USA). Sodium nitrate (NaNO2) and ethyl acetate (EtOAc) were obtained from Junsei (Tokyo, Japan). Sodium carbonate (Na2CO3) and phosphoric acid (H3PO4) were obtained from Samchun Chemical (Seoul, Korea).
이론/모형
- 7 cells were grown in DMEM with 10% FBS and 1% P/S. The cytotoxic effects of CE were determined using the MTT assay. 5×104 cells/well in 96 well plates were treated with CE and incubated for 24 hr.
- Total phenol content was measured using the Folin-Dennis method. In brief, 50 µl of the samples were added to 50 µl of 1 N FC reagent in 96 well plates.
- The total phenol content was measured spectrophotometrically as in the Folin-Dennis method, Prussian blue method, and Vanillin HCl method (Whang et al., 2001). The total phenol content of CE was determined using the Folin-Dennis method and the standard tannic acid calibration curve.
- , 2001). The total phenol content of CE was determined using the Folin-Dennis method and the standard tannic acid calibration curve. The results showed that the total phenol content of CE was increased in a dose-dependent manner (Fig.
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