[논문]흰쥐 심근경색 모델에서 혈장 Atrial Natriuretic Peptide의 변화

안동춘; 김인식

흰쥐 심근경색 모델에서 혈장 Atrial Natriuretic Peptide의 변화
Changes of the Plasma Atrial Natriuretic Peptide during Myocardial Infarction in Rats 원문보기

Journal of veterinary clinics = 한국임상수의학회지, v.29 no.2, 2012년, pp.148 - 153

안동춘 (전북대학교 생체안전성연구소, 수의과대학 해부학교실) , 김인식 (전북대학교 생체안전성연구소, 수의과대학 해부학교실)

초록
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Atrial natriuretic peptide (ANP)은 여러 가지 다양한 심근질환과 연관성이 있는 것으로 알려져 있으나 심근경색에서 ANP의 변화에 대해서는 명확하게 밝혀져 있지 않다. 따라서 본 연구에서는 흰쥐 심근경색 모델을 활용하여 혈장내 ANP의 변화를 살펴보아 심근경색에서 ANP의 역할을 규명해보고자 하였다. 수컷 흰쥐 60일령에서 왼심장동맥을 결찰 하여 심근경색 모델을 만들었고 개흉하여 sham 대조군을 삼았다. 각각의 실험군과 대조군을 수술 후 1, 3, 6, 12, 18시간과 1, 3, 5, 7, 14 및 30일에 희생시켜 실험에 사용하였다. 심근경색의 크기는 planimetry와 perimetry법을 적용하여 측정하였고 혈장내 ANP 농도는 방사면역측정법을 적용하여 측정하였다. 왼심장동맥을 결찰한 실험군에서 평균 심근경색의 크기는 왼심실의 39.6-44.5%이었고 유의성 있는 차이는 없었다. 혈장내 ANP 농도는 심근경색 후 1, 3, 6, 12, 18 및 24시간에 대조군에 비하여 명확하게 증가하였으나 3, 5, 7, 14 및 30일령에서 ANP 농도는 대조군에 비하여 유의성 있는 차이를 나타내지 않았다. 이러한 결과는 수컷 흰쥐에서 혈장내 ANP 농도는 심근경색 초기에 명확하게 증가함을 입증하였고 급성심근경색의 진단을 위한 생체표지인자로 이용할 수 있는 가능성을 제시해주고 있다.

Abstract ▼ AI-Helper

Atrial natriuretic peptide (ANP) is associated with the variety of disorders of myocardial function. The effect, however, of myocardial infarction (MI) on ANP has not been fully described. Thus, this study investigated the effect of experimental MI, induced by left coronary artery ligation, on ANP secretion. Male Sprague-Dawley rats aged 60 d underwent ligation of the left anterior descending coronary artery to induce MI and were compared with a group that underwent a sham operation. Rats of sham operation had a similar procedure without having the suture tightened around the coronary artery. Animals were sacrificed at 1, 3, 6, 12, and 18 h or 1, 3, 5, 7, 14, and 30 d after the procedure. MI size was assessed by planimetry and perimetry, and plasma ANP levels were determined by radioimmunoassay. Mean infarct size was 39.6-44.5% of the left ventricle after coronary occlusion in experimental groups. No significant differences were observed in infarct size among groups. In contrast, the concentration of plasma ANP was significantly higher at 1, 3, 6, 12, 18, and 24 h after left coronary artery ligation than in sham animals. This parameter, however, did not differ significantly at 3, 5, 7, 14, and 30 d after ligation compared with sham-ligated controls. These results demonstrate that plasma ANP levels are markedly increased in the early phase of MI in the male rat and can be a useful biomarker for diagnosis in acute MI.

주제어

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문제 정의

This study observed a marked elevation of circulating ANP in the early phase of MI caused by coronary ligation in rats. ANP levels were highest in rats with the largest MI and the most severe cardiac dysfunction.

제안 방법

Rats were divided into 11 groups of eight rats for analysis at 1, 3, 6, 12, and 18 h and 1, 3, 5, 7, 14, and 30 d after left coronary artery ligation. Sham operated animals were included in groups analyzed at each time point.

데이터처리

Significant differences among the mean values of sham ligation and coronary artery ligation rats within each group were determined by a Student’s t-tests.
Significant differences between the means of different groups were determined by analysis of variance (ANOVA), followed by Duncan's test.

이론/모형

All animals were fed normal rat chow and had access to water ad libitum. This study was carried out in accordance with the Chonbuk National University Animal Experimental Guidelines.

성능/효과

Dogs with chronic renal failure had twice the ANP concentrations of control dogs, while those dogs suffering from congestive heart failure had a 6-fold increase in plasma ANP levels compared to healthy dogs (31). In this study, the concentration of plasma ANP was significantly higher at 1, 3, 6, 12, 18, and 24 h after left coronary artery ligation than in sham animals. This parameter, however, did not differ significantly at 3, 5, 7, 14, and 30 d after ligation compared with sham-ligated controls.

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