[논문]미꾸라지(<tex>$Misgurnus$</tex> <tex>$mizolepis$</tex>)와 미꾸리(<tex>$M.$</tex> <tex>$anguillicaudatus$</tex>) 및 유도된 종간 잡종의 세포유전학적 연구

이승기; 김동수

[국내논문] 미꾸라지($Misgurnus$ $mizolepis$)와 미꾸리($M.$ $anguillicaudatus$) 및 유도된 종간 잡종의 세포유전학적 연구
Cytogenetic Analysis of Reciprocal Hybrids Reveals a Robertsonian Translocation between Mud Loach ($Misgurnus$ $mizolepis$) and Cyprinid Loach ($M.$ $anguillicaudatus$) 원문보기

Korean journal of Ichthyology = 한국어류학회지, v.24 no.1, 2012년, pp.1 - 10

초록
AI-Helper

미꾸라지, 미꾸리 및 상반교배를 통해 유도된 종간 잡종의 세포유전학적 분석을 수행하였다. 미꾸라지와 미꾸리의 염색체 수는 각각 2n=48 (12M+4SM+32A), 2n=50 (10M+4SM+36A)이었고, 잡종군들의 염색체수는 각각 2n=49 (11M+4SM+34A)였다. 모든 그룹의 염색체는 동일한 arm number (NF=64)를 갖고 있었으며, 염색체 다형현상, 암수 간 이형의 염색체는 관찰되지 않았다. 적혈구의 크기, DNA 함량을 분석한 결과 잡종군들은 미꾸라지와 미꾸리의 중간 값을 나타냈다. 염색체의 NORs (nucleolar organizing regions)은 모두 동일한 중부염색체 단완부에서 Ag-positive signal이 나타났다. 이상의 결과는 미꾸라지의 1번 중부 염색체와 미꾸리의 차단부 염색체가 Robertsonian 형의 염색체 전좌 과정을 거쳤을 것을 시사한다.

Abstract ▼ AI-Helper

Reciprocal hybrids between the mud loach ($Misgurnus$ $mizolepis$) and cyprinid loach ($M.$ $anguillicaudatus$) were produced by artificial fertilization. The chromosome number of mud loach was 2n=48, consisting of 12M+4SM+32A chromosomes. The cyprinid loach has 2n=50, consisting of 10M+4SM+36A chromosomes. The chromosome numbers of the diploid reciprocal hybrids were 2n=49, consisting of 11M+4SM+34A chromosomes. All the karyotypes documented in this study had the same arm number of 64. There was no evidence of chromosomal polymorphisms or sex-related heteromorphism. The cytogenetic traits of the hybrid genotypes were intermediate between those of the parent species. In all genotypes, the chromosomal NORs localized to the terminal short arms of the same metacentric chromosome pair. These results suggest that Robertsonian translocation occurred between metacentric chromosome 1 of mud loach and acrocentric chromosome of cyprinid loach.

Keyword

AI 본문요약
AI-Helper

* AI 자동 식별 결과로 적합하지 않은 문장이 있을 수 있으니, 이용에 유의하시기 바랍니다.

문제 정의

The objective of this study was to identify the origin of the haploid complement of chromosomes found in these hybrids and to evaluate the chromosomal NOR phenotypes of the mud loach, the cyprinid loach, and their reciprocal hybrids.

제안 방법

A flow-cytometric analysis was performed to estimate the average cellular DNA content. Blood cells, at a concentration of more than 10⁶ cells mL^-1 , were collected from the caudal veins of 20 individuals per genotype.

대상 데이터

The mud loach and cyprinid loach were obtained from a fish farm located in Jeollabuk-do, Korea. The fish were temporarily maintained in laboratory aquariums at 28℃.

데이터처리

Differences in the genome contents and erythrocyte sizes of the genotypes were tested by one-way analysis of variance (AVOVA) and comparisons between means were made with the Tukey test. Differences were considered significant at P levels of 0.

후속연구

In the present study, cytogenetic results may indicate that Robertsonian translocation occurred in the evolution between the mud loach and cyprinid loach. However, further research is required to determine whether Robertsonian fusion occurred between acrocentric chromosomes in the cyprinid loach. Molecular cytogenetic techniques, such as genomic in situ hybridization, may allow to a more definitive analysis of these reciprocal hybrids and the origins of the parental fishes, when the total genomic DNA from one parent is labeled and hybridized with the fluorescently labeled chromosomes of the other parental fish.

참고문헌 (20)

Animal genome size database. Available from http://www.genomesize.com (accessed October 2011).
Boron, A. 2003. Karyotypes and cytogenetic diversity of the genus Cobitis (Pisces, Cobitidae) in Poland: A review. Cytogenetic evidence for a hybrid origin of some Cobitis triploids. Folia Biol. (Krakow), 51: 49-54.
Cioffi, M.B., C. Martins and L.A. Bertollo. 2010. Chromosome spreading of associated transposable elements and ribosomal DNA in the fish Erythrinus erythrinus. BMC Evol. Biol., 10: 271.

상세보기
Gold, J. 1984. Silver-staining and heteromorphism of chromosomal nucleolus organizer regions in North American cyprinid fishes. Copeia, 1: 232: 5-11.
Hardie, D.C. and P.D.N. Hebert. 2003. The nucleotypic effects of cellular DNA content in cartilaginous and ray-finned fishes. Genome, 46: 683-706.

상세보기
Hashimoto, D.T., P.P. Parise-Maltempi, A. Laudicina, J. Bortolozzi, J.A. Senhorini, F. Foresti and F. Porto-Foresti. 2009. Repetitive DNA probe linked to sex chromosomes in hybrids between Neotropical fish Leporinus macrocephalus and Leporinus elongatus (Characiformes, Anostomidae). Cytogenet. Genome Res., 124: 151-157.

상세보기
Howell, W.M. and D.A. Black. 1980. Localization of the nucleolus organizer regions on the sex chromosomes of the banded killifish, Fundulus diaphanous. Copeia, 3: 544-546.
Kim, D.S., Y.K. Nam and I.S. Park. 1995. Survival and karyological analysis of reciprocal diploid and triploid hybrids between mud loach (Misgurnus mizolepis) and cyprinid loach (Misgurnus anguillicaudatus). Aquaculture, 135: 257-265.

상세보기
Levan, A., K. Fredga and A.A. Sandberg. 1964. Nomenclature for centromeric position on chromosomes. Hereditas, 52: 201-220.

상세보기
Li, Y.J., Y. Tian, M.Z. Zhang, P.P. Tian, Z. Yu, S. Abe and K. Arai. 2010. Chromosome banding and FISH with rDNA probe in the diploid and tetraploid loach Misgurnus anguillicaudatus. Ichthyol. Res., 57: 358-366.

상세보기
Martin, V., P. Rab and H. Kullmann. 2008. Karyotype differentiation in Chromaphyosemion killifishes (Cyprinodontiformes, Nothobranchiidae): patterns, mechanisms and evolutionary implications. Bio. J. Linn. Soc., 94: 143-153.

상세보기
Nelson, J.S. 2006. Fishes of the World (Fourth Edition). John Wiley & Sons, Inc., Hoboken, New Jersey, 146 pp.
Park, I.S., Y.K. Nam and D.S. Kim. 2006. Growth performance, morphometric traits and gonad development of induced reciprocal diploid and triploid hybrids between mud loach (Misgurnus mizolepis Gunter) and cyprinid loach (Misgurnus anguillicaudatus Cantor). Aquacult. Res., 10: 363-371.
Phillips, R.B. and P. Rab. 2000. Chromosome evolution in the Salmonidae (Pisces): An update. Biol. Rev., 76: 1-25.
Phillips, R.B., M.R. Morasch, P.A. Wheeler and G.H. Thorgaard. 2005. Rainbow Trout (Oncorhynchus mykiss) of Idaho and Alaskan Origin (2n58) Share a Chromosome Fusion Relative to Trout of California Origin (2n60). Copea, 3: 661-664.
Porto-Foresti, F., C. Oliveira, Y.A. Tabata, M.G. Rigolino and F. Foresti. 2006. Relationship among growth and different NOR phenotypes in a specific stock of rainbow trout (Oncorhynchus mykiss). Braz. J. Bio., 67: 355-361.
Santi-Rampazzo, A.P., P.B. Nishiyama, P.E.B. Ferreira and I.C. Martins-Santos. 2008. Intrapopulational polymorphism of nucleolus organizer regions in Serrapinnus notomelas (Characidae, Cheirodontinae) from the Parana River. J. Fish Bio., 72: 1236-1243.

상세보기
Sczepanski, T.S., R.B. Noleto, M.M. Cestari and R.F. Artoni. 2010. A comparative study of two marine catfish (Siluriformes, Ariidae): Cytogenetic tools for determining cytotaxonomy and karyotype evolution. Micron, 41: 193-197.

상세보기
Tanaka, M., S. Kimura, T. Fujimoto, S. Sakao, E. Yamaha and K. Arai. 2003. Spontaneous mosaicism occurred in normally fertilized and gynogenetically induced progeny of the kokanee salmon Oncorhynchus nerka. Fish. Sci., 69: 176-180.

상세보기
Vitturi, R., M. Columbia, S. Vizzini, A. Libertini, R. Barbieri and A. Mazeola. 2005. Chromosomal location polymorphism of major rDNA sites in two Mediterranean populations of the killifish Aphanius fasciatus (Pisces: Cyprinodontidae). Micron, 36: 243-246.

상세보기

저자의 다른 논문 :

LOADING...

활용도 분석정보

상세보기

다운로드

내보내기

활용도 Top5 논문

해당 논문의 주제분야에서 활용도가 높은 상위 5개 콘텐츠를 보여줍니다.
더보기 버튼을 클릭하시면 더 많은 관련자료를 살펴볼 수 있습니다.

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format