[논문]온라인 시료주입과 UPLC-Orbitrap 질량분석법을 이용한 수질 조류독소의 고속분석방법 개발 및 환경시료적용

장제헌; 김윤석; 최재원

온라인 시료주입과 UPLC-Orbitrap 질량분석법을 이용한 수질 조류독소의 고속분석방법 개발 및 환경시료적용
Fast and Accurate Determination of Algal Toxins in Water using Online Preconcentration and UPLC-Orbitrap Mass Spectrometry

한국물환경학회지 = Journal of Korean Society on Water Environment, v.28 no.6, 2012년, pp.843 - 850

장제헌 (한국수자원공사 수질분석연구센터) , 김윤석 (한국수자원공사 수질분석연구센터) , 최재원 (한국수자원공사 수질분석연구센터)

Abstract ▼ AI-Helper

Due to the fast response to algae bloom issue in drinking water treatment plant, very fast determination methodology for algal toxin is required. In this study, column switching technique based online preconcentration method was combined with high resolution full scan mass spectrometer to save sample preparation time and to obtain fast and accurate result. After parameter optimization of online preconcentration, 1mL filtered sample was directly injected to trap column with switching valve system. Next, target toxins are eluted by 98% acetonitrile and analysed with 150 - 1,100 amu scan range at 50,000 resolving power. Method detection limit (MDL) for microcystin-LR, the most toxic isomer, was 0.1 ng/mL and others such as microcystin-YR, microcystin-RR and nodularin were 0.08, 0.03 and 0.04 ng/mL, respectively. This is the best improved sensitivities with 1mL volume in the literature. Furthermore, due to the use of ultra pressure HPLC (UPLC), the whole method run was completed in 4 min. Real sample applications for 173 sample including 55 surface water and 118 treatment plant samples for raw and treated water could be done within 16 hours. In our calculation, this methodology is roughly 80% faster than the previous manual solid-phase extraction with LC-MS/MS method.

주제어

참고문헌 (17)

Kim, J. H., Kim, H. C., and Yun, M. A. (2009). Method for simultaneous determination of cyanotoxins in water by LCMS/ MS, Journal of Korean Society on Water Environment, 25(4), pp. 597-605. [Korean Literature]
Lee, J. J., Kim, H. B., Moon, J. S., Lee, J. A., Lee, H. J., Park, H. K., Park, J. H., and Seo, J. K. (2010). Assessment of Microcystin Analysis Methods for Convenient Nonitoring, Korean Society on Water Environment (Fall), pp. 643-644. [Korean Literature]
NIER. (2009). An Assessment of Warning Criteria on Algal Bloom (II), National Institute of Environmental Research, pp. 4-5. [Korean Literature]
Park, H. K., Kim, H. B., Moon, J. S., Lee, J. A., Lee, H. J., Lee, J. J., Park, J. H., Seo, J. K., and Youn, S. J. (2011). Investigation of Criterion on Harmful Algae Alert System using Correlation between Cell Number and Cellular Microcystins Content of Korean Toxic Cyanobacteria, Journal of Korean Society on Water Environment, 27(4), pp. 491-498. [Korean Literature]
Yu, S. J., Han, E. Y., Hwang, J. Y., Ryu, J. K., and Yoon, Y. S. (1999). Analysis of Microcystins in Daecheong Reservior using by High Performance Liquid Chromatography, Journal of Korean Society on Water Environment, 15(4), pp. 517-526. [Korean Literature]
Cong, L., Chena, Q., Huang, B., Lu, B., Ren, Y., and Zhang, J. (2006). Determination of Trace Amount of Microcystins in Water Samples using Liquid Chromatography Coupled with Triple Quadrupole Mass Spectrometry, Nalytica Chimica Acta, 569 (31), pp. 157-168.
Fastner, J., Flieger, I., and Neumann, U. (1998). Optimized Extraction of Microcystins from Field Samples a Comparison of Different Solvents and Procedures, Water Research, 32(10), pp. 3177-3181.

상세보기
Harada, K., Matsuura, K., and Suzuki, M. (1988). Analysis and Purification of Toxic Peptides from Cyanobacteria by Reversed-phase High-performance Liquid Chromatography, Journal of Chromatography A, 448, pp. 275-283.

상세보기
Harada, K. I., Kiyonaga, F., Makoto, S., and Tomoyo, N. (2004). Comprehensive Analysis System using Liquid Chromatography. Mass Spectrometry for the Biosynthetic Study of Peptides Produced by Cyanobacteria, Journal of Chromatography A, 1033, pp. 107-113.

상세보기
Henriksen, A. S. and Olli, K. (1996). Sedimentation and Buoyancy of Aphanizomenon cf. flos-aquac (Nostocales, Cyanophyta) in a Nutrient-replete and Nutrient-depleted Coastal Area of the Baltic Sea, Hycologia, 35, pp. 94-101.
Lawton, L. A., Codd, G. A., and Edwards, C. (1994). Extraction and High-performance Liquid Chromatographic Method for the Determination of Microcystins in Raw and Treated Waters, Analyst, 119, pp. 1525-1530.

상세보기
Lee, H. S., Kim, K., Kim, Y. H., Do, K. S., Jeong, C. K., Lee, H. M., and Choi, S. J. (1999). On Line Trace Enrichment for the Simultaneous Determination of Microcystins in Aqueous Samples using High Performance Liquid Chromatography with Diode-array Detection, Journal of Chromatography A, 848 (1.2), pp. 179-184.
Petrovic, M., Barcelo, D., and Tavazzi, S. (2002). Columnswitching System with Restricted Access Pre-column Packing for an Integrated Sample Cleanup and Liquid Chromatographic - mass Spectrometric Analysis of Alkylphenolic Compounds and Steroid Sex Hormones in Sediment, Journal of Chromatography A, 971(20), pp. 37-45.
Repavich, W. M., Meisner, L. F., Sonzogni, W. C., Standridge, J. H., and Wedepohl, R. E. (1990). Cyanobacteria (blue-green algae) in Wisconsin Waters: Acute and Chronic Toxicity, Water Research, 24, pp. 225-231.

상세보기
Sivonen, K. (2009). Cyanobacterial Toxins. Encyclopedia of Microbiology (Third Edition), pp. 290-307.
WHO. (1999). Toxic Cyanobacteria in Water, A Guide to Their Public Health Consequences, Monitoring and Management, pp. 163-164.
Zweigenbaum, J. A., Beattieb, K. A., Coddb, G. K., and Heniona, J. D. (2000). Direct Analysis of Microcystins by Microbore Liquid Chromatography Electrospray Ionization Ion-trap Tandem Mass Spectrometry, Journal of Pharmaceutical and Biomedical Analysis, 23(4), pp. 723-733.

상세보기

저자의 다른 논문 :

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

AI-Helper ※ AI-Helper는 을 사용합니다.

AI-Helper

안녕하세요, AI-Helper입니다. 좌측 "선택된 텍스트"에서 텍스트를 선택하여 요약, 번역, 용어설명을 실행하세요.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증