Objective: The aim of this study was to investigate and compare the in vivo effects of prostaglandin E2 (PGE2) administered by different methods on orthodontic tooth movement and bone metabolism macroscopically, histopatologically, and biochemically. Methods: Forty-five young adult New Zealand rabbi...
Objective: The aim of this study was to investigate and compare the in vivo effects of prostaglandin E2 (PGE2) administered by different methods on orthodontic tooth movement and bone metabolism macroscopically, histopatologically, and biochemically. Methods: Forty-five young adult New Zealand rabbits were randomly divided into 3 experimental groups (n = 10/group), 1 positive control group (n = 10), and 1 negative control group (n = 5). The experimental rabbits were fitted with springs exerting 20-g reciprocal force on the maxillary incisors and PGE2 (10 ${\mu}g/mL$) was administered by the intravenous, submucosal, or intra ligamentous route aft er appliance insertion and on days 1, 3, 7, and 14 thereafter. All rabbits were sacrificed on day 21 and their premaxillae were resected for histologic evaluation. Results: Tooth movement was observed in the experimental and positive control groups, but the intraligamentous PGE2 group had the highest values of all analyzed parameters, including serum calcium and phosphorus levels and osteoclastic and osteoblastic populations (p < 0.001). Conclusions: Sub mucosal and intraligamentous PGE2 administration significantly increases orthodontic tooth movement and bone metabolism, but the intraligamentous route seems to be more effective.
Objective: The aim of this study was to investigate and compare the in vivo effects of prostaglandin E2 (PGE2) administered by different methods on orthodontic tooth movement and bone metabolism macroscopically, histopatologically, and biochemically. Methods: Forty-five young adult New Zealand rabbits were randomly divided into 3 experimental groups (n = 10/group), 1 positive control group (n = 10), and 1 negative control group (n = 5). The experimental rabbits were fitted with springs exerting 20-g reciprocal force on the maxillary incisors and PGE2 (10 ${\mu}g/mL$) was administered by the intravenous, submucosal, or intra ligamentous route aft er appliance insertion and on days 1, 3, 7, and 14 thereafter. All rabbits were sacrificed on day 21 and their premaxillae were resected for histologic evaluation. Results: Tooth movement was observed in the experimental and positive control groups, but the intraligamentous PGE2 group had the highest values of all analyzed parameters, including serum calcium and phosphorus levels and osteoclastic and osteoblastic populations (p < 0.001). Conclusions: Sub mucosal and intraligamentous PGE2 administration significantly increases orthodontic tooth movement and bone metabolism, but the intraligamentous route seems to be more effective.
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제안 방법
Hundred 40-μm-thick sections were examined by starting with a random single-digit number and analyzing every 10th section thereaft er; for example, beginning with section 7, sections 7, 17, and so on were examined until 10 sections were analyzed.
In this study, the in vivo effects of different routes of PGE2 administration on orthodontic tooth movement and bone metabolism were investigated and compared by macroscopic, histologic, and biochemical evaluations. Intraligamentous and sub mucosal PGE2 administration substantially increased the osteoblastic and osteoclastic populations in the alve olar bone, considerably increasing orthodontic tooth move ment; however, the intraligamentous route was more eff ective.
The aim of the present study was to investigate and compare the in vivo effects of PGE2 administered by different routes on orthodontic tooth movement and bone metabolism macroscopically, histologically, and biochemically.
The experimental animals (n = 10/group) were fitted with the same appliance and injected with 0.06-mL PGE2 (10 μg/mL) in the bilateral auricular veins (intravenous PGE2 group), submucosa immediately distal to the maxillary incisors (submucosal PGE2 group), or PDL surrounding the maxillary incisors (intraligamentous PGE2 group).
대상 데이터
06-mL injection volume was chosen because it corresponded to the minimal injection volume of the intraligamentous injector. PGE2 powder (p5640) was obtained from Sigma-Aldrich (St. Louis, MO, USA) and prepared as a 1 mg/mL stock solution by dissolving in ethanol. This solution was then diluted with a 2% lidocaine solution (2 mL lidocaine and 98 mL distilled water) containing 1:100,000 epinephrine to yield the 10 μg/mL PGE2 solution.
이론/모형
005), the Kruskal-Wallis test was used to assess the differences among the groups. As significant differences were found, the Mann-Whitney U-test was used to determine the pairs of groups responsible for the signifi cant diff erences. Th e data are presented as mean ± standard deviation.
Because the assumption of normality was rejected (p < 0.005), the Kruskal-Wallis test was used to assess the differences among the groups.
, Chicago, IL, USA) was used for statistical analyses. The Kolmogorov-Smirnov test was used to evaluate the data for normality. Because the assumption of normality was rejected (p < 0.
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