Colonizing Ability of Pseudomonas fluorescens 2112, Among Collections of 2,4-Diacetylphloroglucinol-Producing Pseudomonas fluorescens spp. in Pea Rhizosphere원문보기
Kim, Sang-Dal
(School of Biotechnology, Yeungnam University)
,
Fuente, Leonardo De La
(Department of Entomology and Plant Pathology, Auburn University)
,
Weller, David M.
(Department of Plant Pathology, USDA-ARS, Root Disease and Biological Control Research Unit, Washington State University)
,
Thomashow, Linda S.
(Department of Plant Pathology, USDA-ARS, Root Disease and Biological Control Research Unit, Washington State University)
Pseudomonas fluorescens 2112, isolated in Korea as an indigenous antagonistic bacteria, can produce 2,4-diacetylphloroglucinol (2,4-DAPG) and the siderophore pyoveridin2112 for the control of phytophthora blight of red-pepper. P. fluorescens 2112 was classified into a new genotype C among the 17 gen...
Pseudomonas fluorescens 2112, isolated in Korea as an indigenous antagonistic bacteria, can produce 2,4-diacetylphloroglucinol (2,4-DAPG) and the siderophore pyoveridin2112 for the control of phytophthora blight of red-pepper. P. fluorescens 2112 was classified into a new genotype C among the 17 genotypes of 2,4-DAPG producers, by phlD restriction fragment length polymorphism (RFLP). The colonizing ability of P. fluorescens 2112 in pea rhizosphere was equal to the well-known pea colonizers, P. fluorescens Q8r1 (genotype D) and MVP1-4 (genotype P), after 6 cycling cultivations for 18 weeks. Four tested 2,4-DAPG-producing Pseudomonas spp. could colonize with about a 96% dominance ratio against total bacteria in pea rhizosphere. The strain P. fluorescens 2112 was as good a colonizer as other Pseudomonas spp. genotypes in pea plant growth-promoting rhizobacteria.
Pseudomonas fluorescens 2112, isolated in Korea as an indigenous antagonistic bacteria, can produce 2,4-diacetylphloroglucinol (2,4-DAPG) and the siderophore pyoveridin2112 for the control of phytophthora blight of red-pepper. P. fluorescens 2112 was classified into a new genotype C among the 17 genotypes of 2,4-DAPG producers, by phlD restriction fragment length polymorphism (RFLP). The colonizing ability of P. fluorescens 2112 in pea rhizosphere was equal to the well-known pea colonizers, P. fluorescens Q8r1 (genotype D) and MVP1-4 (genotype P), after 6 cycling cultivations for 18 weeks. Four tested 2,4-DAPG-producing Pseudomonas spp. could colonize with about a 96% dominance ratio against total bacteria in pea rhizosphere. The strain P. fluorescens 2112 was as good a colonizer as other Pseudomonas spp. genotypes in pea plant growth-promoting rhizobacteria.
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제안 방법
For the population monitoring of P. fluorescens 2112 in the rhizosphere with KMB3+ rif, spontaneous mutation of the wild-type strain was induced by selecting resistant colonies on LB medium containing 200 µg/ml and 100 µg/ml rifampicin with six cycles of subculturing.
대상 데이터
fluorescens strains were used. P. fluorescens STAD384 (genotype C), P. fluorescens MVP 1-4 (genotype P), and P. fluorescens Q8r1 (genotype D) were obtained from the collections of the USDA-ARS and Washington State University. Genotypes P and D were confirmed to be the best colonizing strains for pea rhizosphere [5].
5 cm mesh screen prior to use. The pea (Pisum sativum L) cultivars used in this study were Columbian and were harvested in 2003 in Washington State.
데이터처리
Comparisons among groups were done by one-way ANOVA (p<0.05).
Different letters at values in rows show significant difference using one-way ANOVA, post hoc tests by Duncan (p<0.05).
이론/모형
The 2,4-DAPG-producing Pseudomonas spp. of rhizosphere or pot soil were determined by the phlD-specific PCR-based TDC method [5]. Soil, rhizosphere soil, or cycled pea roots were harvested in a 50 ml capped tube and the bacteria were extracted with 15 ml of autoclaved distilled water (10 ml for soil).
성능/효과
After the first three cycles, whereas the P2112 inoculated pots displayed 25.9% increased plant weight, the plant weight increase of strains STAD 384, MVP1-4, and Q8r1 was <5% (Fig. 5).
Concerning the population density of the total culturable bacteria in the total rhizosphere, all four strains in the inoculated soil increased to an average of log 8.29 CFU/g after cycle 1, increasing about 100 times from an average of log 6.37 CFU/g at cycle 0. In the case of phlD+, the average population density of the four Pseudomonas spp.
among the biocontrol agents for each area. In this report, a foreign 2,4-DAPG producer was also shown be capable of effectively colonizing roots of pea plants grown in soil recovered from Washington state; the present genotype competed well with genotypes D (Q8r1) and P (MVP1-4). This suggests that foreign-born biocontrolPGPR could colonize the agriculture soil of other countries.
have more affinity for the rhizosphere than other soil bacteria. The average percentage of phlD+ Pseudomonas relative to total bacteria increased from 69.2% at non-plating cycle 0 to 91.9% at cycle 1, 92.2% at cycle 2, 96.7% at cycle 3, and 96.6% at cycle 4 (Table 3). These results indicate that the fluorescent Pseudomonas spp.
The basal population sizes of four inoculated phlD+ strains were determined to be log 4.37 (P2112), log 4.37 (STAD384), log 4.21 (MVP1-4), and 4.68 (Q8r1) CFU/g soil, and phlD+ strain of non-inoculated control soil was log 3.55 CFU/g soil (Fig. 3).
in rhizosphere was suggested to be an important mechanism for plant growth-promoting (PGP) effect. The weight per plant of the upper-ground stem and leaves of the pea plant in P2112-inoculated pots had the highest PGR effect, and the promoting rate was 121.4% compared with that of noninoculated control pot; P. fluorescens STAD384-, MVP1-4-, and Q8r1-inoculated pots displayed a plant weight increase of 103.6%, 106.3%, and 108.1%, respectively. Among the four strains, P.
fluorescens 2112 was the highest populating strain in pea plant rhizosphere. These results support the view that a foreign P. fluorescens 2112 could also exhibit good colonizing potency in soil encountered in Washington state; P. fluorescens Q8r1 increased to log CFU 8.8 (cycle 4) to display the highest population density. In the case of Q8r1, it is conceivable that the strain harmonizes with the indigenous soil bacteria in Quincy soil.
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