[논문]Investigating the Induced Systemic Resistance Mechanism of 2,4-Diacetylphloroglucinol (DAPG) using DAPG Hydrolase-Transgenic Arabidopsis

Chae, Dae-Han; Kim, Da-Ran; Cheong, Mi Sun; Lee, Yong Bok; Kwak, Youn-Sig

doi:10.5423/ppj.oa.02.2020.0031

Investigating the Induced Systemic Resistance Mechanism of 2,4-Diacetylphloroglucinol (DAPG) using DAPG Hydrolase-Transgenic Arabidopsis 원문보기

The plant pathology journal, v.36 no.3, 2020년, pp.255 - 266

Chae, Dae-Han (Division of Applied Life Science (BK21Plus) and Institute of Agriculture & Life Science, Gyeongsang National University) , Kim, Da-Ran (Department of Plant Medicine, Gyeongsang National University) , Cheong, Mi Sun (Division of Applied Life Science (BK21Plus) and Institute of Agriculture & Life Science, Gyeongsang National University) , Lee, Yong Bok (Division of Applied Life Science (BK21Plus) and Institute of Agriculture & Life Science, Gyeongsang National University) , Kwak, Youn-Sig (Division of Applied Life Science (BK21Plus) and Institute of Agriculture & Life Science, Gyeongsang National University)

Abstract ▼ AI-Helper

Plant immune responses can be triggered by chemicals, microbes, pathogens, insects, or abiotic stresses. In particular, induced systemic resistance (ISR) refers to the activation of the immune system due to a plant's interaction with beneficial microorganisms. The phenolic compound, 2,4-diacetylphloroglucinol (DAPG), which is produced by beneficial Pseudomonas spp., acts as an ISR elicitor, yet DAPG's mechanism in ISR remains unclear. In this study, transgenic Arabidopsis thaliana plants overexpressing the DAPG hydrolase gene (phlG) were generated to investigate the functioning of DAPG in ISR. DAPG was applied onto 3-week-old A. thaliana Col-0 and these primed plants showed resistance to the pathogens Botrytis cinerea and Pseudomonas syringae pv. tomato DC3000. However, in the phlG transgenic A. thaliana, the ISR was not triggered against these pathogens. The DAPG-mediated ISR phenotype was impaired in transgenic A. thaliana plants overexpressing phlG, thus showing similar disease severity when compared to untreated control plants. Furthermore, the DAPG-treated A. thaliana Col-0 showed an increase in their gene expression levels of PDF1.2 and WRKY70 but this failed to occur in the phlG transgenic lines. Collectively, these experimental results indicate that jasmonic acid/ethylene signal-based defense system is effectively disabled in phlG transgenic A. thaliana lines.

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표/그림 (6)

그림 Fig. 1. Induction of disease resistance against Botrytis cinerea via 2,4-diacetylphloroglucinol (DAPG)-priming in Arabidopsis.
그림 Fig. 2. Induction of disease resistance against Pst DC3000 via 2,4-diacetylphloroglucinol (DAPG)-priming in Arabidopsis thaliana.
그림 Fig. 3. Disease severity in 2,4-diacetylphloroglucinol (DAPG)-primed Arabidopsis thaliana Col-0 and the phlG transgenic line (D16, H2).
그림 Fig. 4. Disease severity in 2,4-diacetylphloroglucinol (DAPG)-primed Arabidopsis thaliana Col0 and phlG transgenic lines (D16, H2).
그림 Fig. 5. Salicylic acid and jasmonic acid/ethylene signaling genes' expression in Arabidopsis thaliana Col-0, D16, and H2 plants after challenging them with a fungal pathogen.
그림 Fig. 6. Salicylic acid and jasmonic acid/ethylene signaling genes' expression in Arabidopsis thaliana Col-0, D16, and H2 plants after challenging them with a bacterial pathogen.

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