서울 근교의 민물 습지(FW), 해수습지(SW), 산림 토양(FS) 그리고 매립지 복토(LS)의 메탄산화세균 군집을 clone library/sequencing 기법을 이용하여 분석하였다. 메탄산화세균인 Methylocaldum, Methlyococcus과 Methylosinus는 FS와 SW에서 풍부하였으며, Methylobacter와 Methylomonas는 FW에서 풍부하였고, Methylocystis와 Methylomicrobium은 LS에서 우점하였다. 메탄 산화가 관찰되기 전까지 필요한 lag phase는 각 토양별로 유의적으로 차이가 있었고, 메탄 산화속도는 $FW{\geq}LS{\geq}SW>FS$순이었다. 이러한 결과들은 토양의 환경조건은 메탄산화세균의 군집과 메탄산화능에 영향을 미치는 중요한 인자임을 시사한다.
서울 근교의 민물 습지(FW), 해수습지(SW), 산림 토양(FS) 그리고 매립지 복토(LS)의 메탄산화세균 군집을 clone library/sequencing 기법을 이용하여 분석하였다. 메탄산화세균인 Methylocaldum, Methlyococcus과 Methylosinus는 FS와 SW에서 풍부하였으며, Methylobacter와 Methylomonas는 FW에서 풍부하였고, Methylocystis와 Methylomicrobium은 LS에서 우점하였다. 메탄 산화가 관찰되기 전까지 필요한 lag phase는 각 토양별로 유의적으로 차이가 있었고, 메탄 산화속도는 $FW{\geq}LS{\geq}SW>FS$순이었다. 이러한 결과들은 토양의 환경조건은 메탄산화세균의 군집과 메탄산화능에 영향을 미치는 중요한 인자임을 시사한다.
Methanotrophic communities from freshwater wetland (FW), seawater wetland (SW), forest (FS), and landfill soils (LS) around Seoul of South Korea, were characterized using comparative sequence analyses of clone libraries. Proportions of Methylocaldum, Methlyococcus and Methylosinus were found to be g...
Methanotrophic communities from freshwater wetland (FW), seawater wetland (SW), forest (FS), and landfill soils (LS) around Seoul of South Korea, were characterized using comparative sequence analyses of clone libraries. Proportions of Methylocaldum, Methlyococcus and Methylosinus were found to be greater in FW and SW, while Methylobacter and Methylomonas were more notable in FS and Methylocystis and Methylomicrobium more prominent in LS. Lag periods behind the initiation of methane oxidation significantly varied amongst the soils. Methane oxidation rates were greater in $FW{\geq}LS{\geq}SW>FS$ (p<0.05). Thus, the environmental setting is a significant factor influencing the communities and capabilities of methanotrophs.
Methanotrophic communities from freshwater wetland (FW), seawater wetland (SW), forest (FS), and landfill soils (LS) around Seoul of South Korea, were characterized using comparative sequence analyses of clone libraries. Proportions of Methylocaldum, Methlyococcus and Methylosinus were found to be greater in FW and SW, while Methylobacter and Methylomonas were more notable in FS and Methylocystis and Methylomicrobium more prominent in LS. Lag periods behind the initiation of methane oxidation significantly varied amongst the soils. Methane oxidation rates were greater in $FW{\geq}LS{\geq}SW>FS$ (p<0.05). Thus, the environmental setting is a significant factor influencing the communities and capabilities of methanotrophs.
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제안 방법
In contrast, methanotrophs in wetland or landfill soils may have lower enzymatic affinity levels since they grow on methane produced in the soils or emitted from the landfills, respectively. The main objective in the present study was to test hypotheses that environmental setting can determine whether a specific type of methanotrophs is present, and that it also can determine methane-oxidation potential of methanotrophs.
대상 데이터
oxidation of four different soil environments. Four regions with different environmental settings in the suburbs of Seoul, South Korea, were selected for soil sampling: landfill (Gapyeong, Gyeonggi province), freshwater wetland (Namyanju, Gyeonggi), seawater wetland (Yeongjong island, Incheon) and forest (Ungil mountain, Gyeonggi) in Korea. Five subsamples were collected at a depth of 10 cm from each soil site.
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