[논문]Antioxidant activities and determination of phenolic compounds isolated from oriental plums (Soldam, Oishiwase and Formosa)

Kim, Se-Na; Kim, Mee-Ree; Cho, Soo-Muk; Kim, So-Young; Kim, Jung-Bong; Cho, Young-Sook

doi:10.4162/nrp.2012.6.4.277

Antioxidant activities and determination of phenolic compounds isolated from oriental plums (Soldam, Oishiwase and Formosa) 원문보기

Nutrition research and practice, v.6 no.4, 2012년, pp.277 - 285

Kim, Se-Na (Department of Agrofood Resources, NAAS, RDA) , Kim, Mee-Ree (Department of Food and Nutrition, Chungnam National University) , Cho, Soo-Muk (Department of Agrofood Resources, NAAS, RDA) , Kim, So-Young (Department of Agrofood Resources, NAAS, RDA) , Kim, Jung-Bong (Department of Agrofood Resources, NAAS, RDA) , Cho, Young-Sook (Department of Agrofood Resources, NAAS, RDA)

Abstract ▼ AI-Helper

The purposes of this study were to determine phenolic compounds and to evaluate antioxidant activities of plums (Soldam, Oishiwase and Formosa). Soldam contains the highest amount of total phenolics among cultivars (Formosa: 4.0%, Oishiwase: 3.3%, Soldam: 6.4% for total phenolic) as well as the total flavonoids of which constituents were mainly myricetin and anthocyanidin. The antioxidant activities were measured by DPPH, ABTS radical scavenging, and SOD-like activities. The DPPH radical scavenging activity of Korean plum extracts (200 ${\mu}g/mL$) showed more than 43%, and the Soldam turned out to be the highest : ID50 value: 160-177 ${\mu}g/mL$ for Formosa and Oishiwase; 58-64 ${\mu}g/mL$ for Soldam. The ABTS radical scavenging activity of Korean plum extracts (200 ${\mu}g/mL$) was found to be more than 50%. The SOD-like activity of Korean plum extracts (200 ${\mu}g/mL$) showed more than 70%. Among three kinds of cultivars, Soldam had the highest antioxidant activity. The nitrite scavenging activity of Soldam was 61.5%, which is the highest, compared with that of the other cultivars, about 50%. From these results, Korean plums turned out to be phytochemical rich fruit as well as to show high antioxidant activities.

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가설 설정

1) Ultrasonification extracts at 60℃
3) The BHT is used at the concentration of 25 μg/mL (n = 3).

제안 방법

In this experiment, polyphenol contents of Formosa, Oishiwase and Soldam were analyzed by using Folin-denish and the result are shown in Fig. 1A. Total polyphenol content of Soldam 6.
In this study, we determinated the phenolic compounds and evaluated antioxidant activities of oriental plums (Soldam, Oishiwase and Formosa). Total polyphenols widely distributed in the plant as one of the secondary metabolites that has various structures and molecular weight.
However these studies are specific to some varieties of plum, it is not enough for the overall study on major varieties of oriental plums. The objectives in this study were to assess antioxidant capacity, to determine total phenolic compound content and identify the configuration of phenolic compound of various varieties of plums.

데이터처리

Statistical comparisons were performed via one-way analysis of variance (ANOVA) followed by Duncan’s multiple range test.

이론/모형

Nitrite scavenging ability was measured according to the method of Gray and Dugan [34]. The 1 mL of sample added 1 mL of 1 mM NaNO₂ was adjusted to pH 1.
Total polyphenolic content was determined according to the method of Folin-denish [30]. The reaction was mixed to 1 mL of extracted sample, 10 mL of 10% Na₂CO₃ and 1mL of 1N-Folin-Ciocalteau’s phenol reagent.

성능/효과

5%. The highest scavenging abilities of Soldam obtained by stirring and ultrasonification extraction were in 61.5 and 50.3%, respectively.
There was no significant difference between as Formosa and Oishiwase depending on extraction method and the activity value at the 200 μg/mL concentration of Formosa ranged from low level of 73 to 76%, Oishiwase ranged from low level of 72 to 76%.
These results of DPPH radical Scavenging activity experiment, there was no significant difference between as Formosa and Oishiwase well as extraction method (Table 3, Fig. 4). But Soldam showed the highest DPPH radical scavenging activity and that was concentration-dependent.
1A. Total polyphenol content of Soldam 6.4% Formosa 4.3%, and Oishiwase 3.3% of the 3 different varieties that were subjected in this study. Compared with polyphenol content of domestic commercial fruit as like 2.

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