[논문]Leaf Rot and Leaf Ring Spot Caused by Rhizoctonia solani in Chinese Cabbage

Shim, Chang-Ki; Kim, Min-Jeong; Kim, Yong-Ki; Jee, Hyeong-Jin; Hong, Sung-Jun; Park, Jong-Ho; Han, Eun-Jung; Yun, Jong-Chul

doi:10.5423/rpd.2013.19.4.300

Leaf Rot and Leaf Ring Spot Caused by Rhizoctonia solani in Chinese Cabbage 원문보기

Research in plant disease = 식물병연구, v.19 no.4, 2013년, pp.300 - 307

Shim, Chang-Ki (Organic Agriculture Division, National Academy of Agricultural Science, Rural Development Administration) , Kim, Min-Jeong (Organic Agriculture Division, National Academy of Agricultural Science, Rural Development Administration) , Kim, Yong-Ki (Organic Agriculture Division, National Academy of Agricultural Science, Rural Development Administration) , Jee, Hyeong-Jin (Organic Agriculture Division, National Academy of Agricultural Science, Rural Development Administration) , Hong, Sung-Jun (Organic Agriculture Division, National Academy of Agricultural Science, Rural Development Administration) , Park, Jong-Ho (Organic Agriculture Division, National Academy of Agricultural Science, Rural Development Administration) , Han, Eun-Jung (Organic Agriculture Division, National Academy of Agricultural Science, Rural Development Administration) , Yun, Jong-Chul (Organic Agriculture Division, National Academy of Agricultural Science, Rural Development Administration)

Abstract ▼ AI-Helper

This study was conducted to determine the occurrence of leaf rot and leaf ring spot, caused by Rhizoctonia solani in Chinese cabbage under seedling nursery and cultivation greenhouses. Symptoms of leaf rot and leaf ring spot were found in three Chinese cabbage cultivars, Brassica campestris subsp. pekinensis, 'Ryeokgwang', 'Daetong', and 'CR mat'. In Hwacheon, the disease incidence was 73.8% in the seedling stage of the Chinese cabbage. In Icheon, the symptoms were observed on the upper leaves of the Chinese cabbage cultivar, 'Norangmini' with 20.5% of disease incidence. The symptoms appeared as primary lesions consisting of small, circular necrotic ring spots with gray color, 1.4-3.0 mm in diameter, accompanied by secondary rot lesions with large irregular borders of leaves. The color of mycelial mat of 20 isolates was dark brown and light brown. The average hyphal diameter of all the isolates was within 5.01-11.12 ${\mu}m$. Among the 20 strains isolated from Chinese cabbage, 16 isolates and four isolates anastomosed with the AG-1 (IB) and AG-1 (IC), respectively. Twenty isolates tested were only virulent on foliage parts of Chinese cabbage leaves but were avirulent on stem parts of the plants. Based on the mycological characteristics and pathogenicity test on host plants, the fungus was identified as Rhizoctonia solani.

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문제 정의

This paper will discuss the incidence and the causal organism of leaf rot and leaf ring spot diseases of Chinese cabbage under the organic Chinese cabbage seedling nursery and cultivation greenhouses in Hwacheon and Icheon.

제안 방법

This study identified twenty isolates of R. solani obtained from organic Chinese cabbage fields by morphology and anastomosis grouping. R.

대상 데이터

3). Ten isolates collected from Hwacheon anastomosed with AG-1 (IB). From Icheon, four isolates were anastomosed with AG-1 (IB) and six isolates anastomosed with AG-1 (IC) (Table 4, Fig.
solani. Tester strains of multinucleate Rhizoctonia including AG-1 (IA), AG1 (IB), AG-1 (IC), AG-2-I, AG-2-2 (IIIB), AG-2-2 (IV), AG-3, AG-4 and unidentified two strains, R. cerealis, and R. edophytica were used. Single 5-mm-diameter agar disc was cut from the perimeter of a 2- to 3-dayold colony of each isolate on PDA.
Disease incidence. The study was conducted in 2011 at the three seedling nursery greenhouses in Hwacheon and ten cultivation greenhouses in Icheon. Chinese cabbage with leaf rot and leaf ring spot symptoms were visually determined and recorded based on the percentage of diseased plants in 20 replicates of each greenhouse one week before transplanting and harvesting time, respectively, in the seedling nursery and cultivation greenhouses.
Twenty isolates of R. solani were tested for pathogenic potential on Chinese cabbage, ‘Daetong’ with 15 seedlings for each isolate.

이론/모형

edophytica were obtained from the Korean Agricultural Culture Collection (KACC) (Table 1). The determination of the anastomosis group of the isolates was determined by using the methodology of Kim et al. (1993). Twenty isolates of R.

성능/효과

After four days of growth on PDA, white sclerotial initials were visible and after at eight days, all sclerotia of 20 isolates were dark brown and showed small (0.38−1.28 mm) and uniform of sclerotia formation on PDA.
It was concluded that on the basis of mycological characteristics and pathogenicity test on host plants, the twenty isolates were identified as R. solani AG-1 (IB) and AG-1 (IC).
9% for ‘CR mat’ (Table 2). One week before transplanting, leaf rot and leaf ring spot symptoms of three Chinese cabbage cultivars were investigated having 73.8% of disease incidence (Fig. 1).
The results indicated that only leaf rot and leaf ring spot symptoms were observed on the foliage part of Chinese cabbage of seedlings and plants in Hwacheon seedling nursery and Icheon cultivation greenhouse. Earlier researches showed that most bottom rot pathogen, R.

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