[논문]Biological Control of Apple Ring Rot on Fruit by Bacillus amyloliquefaciens 9001

Li, Yan; Han, Li-Rong; Zhang, Yuanyuan; Fu, Xuechi; Chen, Xinyi; Zhang, Lixia; Mei, Ruhong; Wang, Qi

doi:10.5423/ppj.si.08.2012.0125

Biological Control of Apple Ring Rot on Fruit by Bacillus amyloliquefaciens 9001 원문보기

The plant pathology journal, v.29 no.2, 2013년, pp.168 - 173

Li, Yan (Key Laboratory of Plant Pathology, Ministry of Agriculture, Department of Plant Pathology, China Agricultural University) , Han, Li-Rong (Research and Development Center of Biorational Pesticides, Northwest A & F University) , Zhang, Yuanyuan (Key Laboratory of Plant Pathology, Ministry of Agriculture, Department of Plant Pathology, China Agricultural University) , Fu, Xuechi (Key Laboratory of Plant Pathology, Ministry of Agriculture, Department of Plant Pathology, China Agricultural University) , Chen, Xinyi (Key Laboratory of Plant Pathology, Ministry of Agriculture, Department of Plant Pathology, China Agricultural University) , Zhang, Lixia (China Green Health Agriculture (Beijing) Biotechnology Co., Ltd.) , Mei, Ruhong (Key Laboratory of Plant Pathology, Ministry of Agriculture, Department of Plant Pathology, China Agricultural University) , Wang, Qi (Key Laboratory of Plant Pathology, Ministry of Agriculture, Department of Plant Pathology, China Agricultural University)

Abstract ▼ AI-Helper

Apple ring rot disease, caused by Botryosphaeria dothidea (Moug. ex. Fr) Ces. et de Not., is one of the most important diseases on apple fruits. In this study, strain 9001 isolated from healthy apple fruits from an infested orchard was evaluated for its biocontrol activity against apple ring rot in vitro and in vivo. Strain 9001 showed obvious antagonistic activity to B. dothidea YL-1 when plated on potato dextrose agar. Soaking healthy apples in the bacterial suspensions of strain 9001 prior to artificial inoculation of fungal pathogen resulted in a dramatic decrease in disease incidence when compared to the control. Moreover, either field application in the growth season or postharvest treatment of apples from infected orchards with bacterial suspensions of strain 9001 resulted in significantly reduced disease incidence within the storage period for 4 months at room temperature. Based on the phylogenetic analysis of 16S rRNA and the gyrA gene, strain 9001 was identified as Bacillus amyloliquefaciens. These results indicated that B. amyloliquefaciens 9001 could be a promising agent in biocontrol of apple ring rot on fruit, which might help to minimize the yield loss of apple fruit during the long postharvest period.

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dothidea, and situated at Beiliu village, Changping District, Beijing, China. In the orchard, eight trees were chosen for the biocontrol trial. Bacterial suspensions of strain 9001 at the concentration of 10⁸ cfu/mL were sprayed on four trees on May 11, June 5, June 30 and Sept.

성능/효과

Phylogenetic identification of strain 9001 based on 16S rRNA and gyrA gene sequence analysis. Based on 16S rRNA gene sequence analysis, an NCBI BLAST search yielded a close similarity to B. amyloliquefaciens strain DM09 and B. subtilis strain M16K 16S rRNA, indicating that strain 9001 belongs to the species B. amyloliquefaciens or B. subtilis. This was clarified by the sequence of the gyrase gene gyrA, which has previously been shown to be effective for resolving these closely related taxa of the B.
In conclusion, B. amyloliquefaciens 9001 was found to have effective biocontrol activity against apple ring rot in vitro and in vivo. Moreover, either field application in the growth season or postharvest treatment of apples from infected orchard with bacterial suspensions of B.
Hence, an assay involving spores of pathogens and isolates were expected to give more realistic picture than dual culture assay. In our results, the supernatant of B. amyloliquefaciens 9001 cell culture showed a strong inhibition of spore germination of the pathogen B. dothidea YL1 in vitro (Table 2). This result is consistent with previous studies reporting that B.
Therefore, after being immersed in different concentrations of Bacillus strain 9001, apples were inoculated with fungal spores at the concentration of 10⁵ spores/mL. The data showed that apples treated with strain 9001 at the concentration of either 10⁶ or10⁷ cfu/mL showed no symptoms until 37 days after inoculation (Table 2). In contrast, apples treated with sterile distilled water showed rot symptoms 20 days post inoculation (Table 2).
amyloliquefaciens 9001 resulted in significantly reduced disease incidence in the storage period. These results indicated that B. amyloliquefaciens 9001 could be a promising agent in biocontrol of apple ring rot on fruit. Further research is required to explore its mechanism of biocontrol.

후속연구

amyloliquefaciens 9001 could be a promising agent in biocontrol of apple ring rot on fruit. Further research is required to explore its mechanism of biocontrol.
is also considered to play a key factor in the biological control activity (Janisiewicz and Korsten, 2002). Further research is required to explore this mechanism of biocontrol.
However, its effect on apple ring rot disease is uncertain. The objective of the present study was to evaluate the biological control activity in vitro and in vivo and to phylogenetically identify it based on 16S rRNA and gyrA gene sequence analysis. The results of our study may provide an alternative to the present methods of prevention to control the apple ring rot during the storage period.
The objective of the present study was to evaluate the biological control activity in vitro and in vivo and to phylogenetically identify it based on 16S rRNA and gyrA gene sequence analysis. The results of our study may provide an alternative to the present methods of prevention to control the apple ring rot during the storage period.

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