[논문]Association of FASN and SCD genes with fatty acid composition in broilers

Maharani, Dyah; Seo, Dong-Won; Choi, Nu-Ri; Jin, Shil; Cahyadi, Muhammad; Jo, Cheorun; Lee, Jun-Heon

doi:10.7744/cnujas.2013.40.3.215

Association of FASN and SCD genes with fatty acid composition in broilers 원문보기

농업과학연구 = CNU Journal of agricultural science, v.40 no.3, 2013년, pp.215 - 220

Maharani, Dyah (Department of Animal Science and Biotechnology, Chungnam National University) , Seo, Dong-Won (Department of Animal Science and Biotechnology, Chungnam National University) , Choi, Nu-Ri (Department of Animal Science and Biotechnology, Chungnam National University) , Jin, Shil (Department of Animal Science and Biotechnology, Chungnam National University) , Cahyadi, Muhammad (Department of Animal Science and Biotechnology, Chungnam National University) , Jo, Cheorun (Department of Animal Science and Biotechnology, Chungnam National University) , Lee, Jun-Heon (Department of Animal Science and Biotechnology, Chungnam National University)

Abstract ▼ AI-Helper

Fatty acids (FAs) were considered in activating nuclear hormone receptors that play significant roles in the cellular lipid metabolism by the regulation of several genes. Previously, fatty acid synthase (FASN) and stearoyl-CoA desaturase (SCD) genes have been known to regulating the FA metabolism. In this study, associations of FASN and SCD genes with fatty acid (FA) composition in broilers were investigated. Tissue samples from 95 Cobb 500 broilers were used for DNA extraction. The g.1222 A>G SNP located in intron 42 of FASN gene and 2 SNPs in SCD gene, one in exon 2 (g.3728A>G) and the other in exon 4 (g.12903G>A), were subjected for genotyping using PCR-RFLP method. One of the SNPs in SCD gene, SNP g.3728A>G had significant association with myristoleic acid (C14:1; P<0.05), palmitic acid (C16:0; P<0.05), palmitoleic acid (C16:1; P<0.05) and saturated FA (SFA; PG in FASN gene had only suggestive association with arachidic acid (C20:0; P=0.08). The findings in this study suggest that the SNP in exon 2 of SCD gene can be used as a molecular marker for selecting birds having desirable FA composition in broilers.

주제어

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문제 정의

However, none of study has been reported the polymorphisms of FASN and SCD gene and their associations with fatty acid composition in chicken. Therefore, this study aimed to identify the relationships between the polymorphisms in both FASN and SCD genes with fatty acid compositions in broilers.

제안 방법

1222 A>G SNP of FASN gene and fatty acid composition, this is the first report to investigate the SNP in FASN gene and the association test with fatty acid composition.
Amplifications were performed for 10 min at 94℃, 35 cycles of 30 s at 94℃, 30 s at the annealing temperature, and 30 s at 72℃, and a final extension of 10 min at 72℃ using either a GeneAmp PCR system 2700 (Applied Biosystems, Franklin Lakes, NJ, USA) or a C1000™ Thermal Cycler(BioRad Laboratories, Hercules, CA, USA).

대상 데이터

Table 2 shows the descriptive statistics for fatty acid composition in broiler chicken. Fourteen FAs including total SFA, PUFA and MUFA were detected in each sample. Total SFA contained four FAs, namely myristic acid (C14:0), palmitic acid (C16:0) stearic acid (C18:0) and arachidic acid (C20:0), with an average level of 0.
Ninety five Cobb 500 broilers were used in this study. They were reared under the same feeding conditions until 28 days of age which had approximately 1.

데이터처리

The effects of FASN and SCD genotypes on FA composition traits were tested using the general linear model (GLM) in the SPSS ver. 17.0 program(SPSS, Chicago, IL, USA). In order to test the pairwise differences between the effects of genotypes, Tukey’s test was also performed.

이론/모형

The SNP g.37284A>G and g.12903G>Alocated in exon 2 and 4, respectively, were used for genotyping using PCR-RFLP methods.
00(Tom Hall, Ibis Therapeutics, California, USA) and the single nucleotide polymorphisms (SNPs) were confirmed based on the electrophoregram results. The SNPs in FASN and SCD genes were genotyped by the PCR-restriction fragment length polymorphism(PCR-RFLP) method (Table 1). The restriction enzyme digestion was performed in 20 µl reaction volumes with approximately 15 µl of PCR products and 2 units of each restriction enzyme.

성능/효과

In the case of SCD gene, the SNPs were manually identified by direct sequencing using fifteen chicken DNA samples. As the results, five SNPs were detected in two exons (exon 2 and 4) and two introns (intron 2 and 5). The SNP g.
As the results, the SNP g.3728A>G in SCD gene had significant association with myristoleic acid (C14:1; P<0.05), palmitic acid (C16:0; P<0.05), palmitoleic acid (C16:1; P<0.05) and saturated FA (SFA; P<0.05).
The detailed analysis revealed that the g.3728A>G SNP of SCD gene was significantly associated with myristoleic acid (C14:1; P-value=1.1x10-2), palmitic acid (C16:0; P-value=2.4x10-2), palmitoleic acid (C16:1; P-value=4.2x10-2) and saturated FA (SFA; P-value=3.5x10-2).
5x10^-2). The results indicated that the identified SNP was associated with an increase in palmitic acid and SFA but decrease in myristoleic acid in heterozygote animals. Moreover, the effect of the SNP in GG homozygous animals indicated the increase in palmitoleic acid.

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