Objectives: Bletillae Rhizoma, the roots of Bletilla striata, is used to restrain the leakage of blood and stop bleeding. It can cure the sores, ulcers, and chapped skin. This study was designed to investigate the collagen metabolism, elastase and tyrosinase activity of Bletillae Rhizoma extract (BR...
Objectives: Bletillae Rhizoma, the roots of Bletilla striata, is used to restrain the leakage of blood and stop bleeding. It can cure the sores, ulcers, and chapped skin. This study was designed to investigate the collagen metabolism, elastase and tyrosinase activity of Bletillae Rhizoma extract (BR). Methods : The effects of BR on type I procollagen production and collagenase activity in human normal fibroblasts Hs68 after UVB (312 nm) irradiation were measured by ELISA method. The elastase activity, tyrosinase activity, and L-DOPA oxidation after treatment of BR were measured as well. Results : In the present study, the collagen production (type I procollagen) was significantly increased to $15.7{\pm}1.8$ ng/ml at a concentration of BR 100 ${\mu}g/ml$ in UVB damaged Hs68 cells. The increased collagenase activity after UVB damage was significantly recovered to $42.7{\pm}0.7%$, $54.5{\pm}3.5%$, and $38.4{\pm}0.9%$ by BR 10, 30, and 100 ${\mu}g/ml$. The activities of BR 10 mg/ml on tyrosinase activity was significantly reduced to $45.1{\pm}8.4%$ as well. However, there were no significant effects on the elastase activity and the L-DOPA oxidation. Conclusion : BR showed the promoting effects of collagen synthesis and inhibitory effects of collagenase activity in Hs68, human normal fibroblast cells. And these could be thought to have the anti-wrinkle effects and whitening effects in vitro. These results suggest that BR may have potential as an anti-aging ingredient in cosmetic treatment.
Objectives: Bletillae Rhizoma, the roots of Bletilla striata, is used to restrain the leakage of blood and stop bleeding. It can cure the sores, ulcers, and chapped skin. This study was designed to investigate the collagen metabolism, elastase and tyrosinase activity of Bletillae Rhizoma extract (BR). Methods : The effects of BR on type I procollagen production and collagenase activity in human normal fibroblasts Hs68 after UVB (312 nm) irradiation were measured by ELISA method. The elastase activity, tyrosinase activity, and L-DOPA oxidation after treatment of BR were measured as well. Results : In the present study, the collagen production (type I procollagen) was significantly increased to $15.7{\pm}1.8$ ng/ml at a concentration of BR 100 ${\mu}g/ml$ in UVB damaged Hs68 cells. The increased collagenase activity after UVB damage was significantly recovered to $42.7{\pm}0.7%$, $54.5{\pm}3.5%$, and $38.4{\pm}0.9%$ by BR 10, 30, and 100 ${\mu}g/ml$. The activities of BR 10 mg/ml on tyrosinase activity was significantly reduced to $45.1{\pm}8.4%$ as well. However, there were no significant effects on the elastase activity and the L-DOPA oxidation. Conclusion : BR showed the promoting effects of collagen synthesis and inhibitory effects of collagenase activity in Hs68, human normal fibroblast cells. And these could be thought to have the anti-wrinkle effects and whitening effects in vitro. These results suggest that BR may have potential as an anti-aging ingredient in cosmetic treatment.
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대상 데이터
Bletillae Rhizoma was purchased from Omniherb (Korea). Bletillae Rhizoma extract (BR) was prepared as follow.
데이터처리
Significances of changes were evaluated using one‐way analysis of variance (ANOVA) with Dunnett’s post hoc test using the SPSS ver. 10.1 (SPSS Inc., USA).
이론/모형
The elastase activity was evaluated by using a modification of a previously reported method of Kraunsoe et al6). In order to evaluate the inhibition of elastase activity, the amount of released p‐nitroaniline, which was hydrolyzed from the substrate, N‐succinyl‐ Ala‐Ala‐Ala‐p‐nitroanilide, by elastase, was read with a maximum absorbance at 410 nm7).
The inhibitory effect of BR on L‐DOPA oxidation was determined according to the method of Joshi et al9). with a slight modification.
성능/효과
In conclusion, BR showed the inhibitory effects on collagenase and slightly increased the synthesis of collagen. The inhibitory effect of BR on elastase was not found out.
후속연구
It is some hints, which might be interpreted as suggesting the possibility of the usage for MMP-1 enzyme inhibitor. However, further research should be performed.
참고문헌 (15)
Bensky D, Clavey S, Stoger E. Materia Medica 3rd Ed. WA : Eastland Press. 2004 : 585-8.
Kim IR, Kim HC, Kuk YB, Park SJ, Park YK, Park JH, Seo BI, Seo YB, Song HJ, Shin MK, Lee YJ, Lee YC, Lee JH, Leem KH, Cho SI, Chung JK, Joo YS, Choi HY. BonchoHak. Seoul : YoungLim Press. 2007 : 428-9.
Emilia M, Luca S, Francesca B, Luca B, Paolo S, Giuseppe F, Gianbattista B, Carmela M, Luigi M, Mauro L. Topical hemostatic agents in surgical practice. Transfus Apher Sci. 2011 ; 45(3) : 305-11.
Kraunsoe JA, Claridge TD, Lowe G. Inhibition of human leukocyte and porcine pancreatic elastase by homologues of bovine pancreatic trypsin inhibitor. Biochemistry. 1996 ; 35(28) : 9090?6.
Kim YH, Chung CB, Kim JG, Ko KI, Park SH, Kim JH, Eom SY, Kim YS, Hwang YI, Kim KH. Antiwrinkle activity of ziyuglycoside I isolated from a Sanguisorba officinalis root extract and its application as a cosmeceutical ingredient. Biosci Biotechnol Biochem. 2008 ; 72(2) : 30311.
Vanni A, Gastaldi D, Giunata G. Kinetic investigations on the double enzyme activity of the tyrosinase mushroom. Ann Chim. 1990 ; 80 : 35-60.
Joshi PC, Carraro C, Pathak MA. Involvement of reactive oxygen species in the oxidation of tyrosine and dopa to melanin and in skin tanning. Biochem Biophys Res Commun. 1987 ; 142(1) : 26574.
Yang ZY, Guon GX, Lin ZF. Elastolytic activity from Flavobacterium odoratum. Microbial screening and cultivation, enzyme production and purification. Process Biochem. 1994 ; 29(6) : 427-36.
Di Lullo GA, Sweeney SM, Korkko J, AlaKokko L, San Antonio JD. Mapping the ligandbinding sites and diseaseassociated mutations on the most abundant protein in the human, type I collagen. J Biol Chem. 2002 ; 277(6) : 422331.
Shin JY, Hur W, Wang JS, Jang JW, Kim CW, Bae SH, Jang SK, Yang SH, Sung YC, Kwon OJ, Yoon SK. HCV core protein promotes liver fibrogenesis via upregulation of CTGF with TGFbeta1. Exp Mol Med. 2005 ; 37(2) : 13845.
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