[논문]B16/F10 흑색종양세포에서 삼내자 메탄올 추출물의 멜라닌 생성에 미치는 억제효과

윤정원; 한정민; 윤화정; 고우신

doi:10.6114/jkood.2013.26.1.001

[국내논문] B16/F10 흑색종양세포에서 삼내자 메탄올 추출물의 멜라닌 생성에 미치는 억제효과
Inhibitory Effects of Methanol Extract of Kaempferia galanga on melanogenesis in B16/F10 Melanoma Cells 원문보기

韓方眼耳鼻咽喉皮膚科學會誌 = The journal of Korean Medicine Ophthalmology & Otolaryngology & Dermatology, v.26 no.1, 2013년, pp.1 - 18

윤정원 (동의대학교 한의과대학 안이비인후피부과학교실) , 한정민 (동의대학교 한의과대학 안이비인후피부과학교실) , 윤화정 (동의대학교 한의과대학 안이비인후피부과학교실) , 고우신 (동의대학교 한의과대학 안이비인후피부과학교실)

Abstract ▼ AI-Helper

Objective: Recently the demands for the effective and safe depigmentative and anti-aging agents of the skin have increased due to the medical, pharmaceutical and cosmetic reasons. The purpose of this study is to investigate the MKG(Methanol Extract of Kaempferia galanga) and their dermal bioactivity properties related to cosmeceuticals such as depigmentation. Methods: We assessed inhibitory effects of MKG on melanin production in B16/F10 melanoma cells, on mushroom tyrosinase activity, effects of MKG on the expression tyrosinase, TRP-1, TRP-2, GSK-$3{\beta}$, CREB, MITF in B16/F10 melanoma cells without cytotoxicity range. Cell viability was measured by MTT assay and tyrosinase activity was assessed using by DOPA staining, western-blot analysis. We measured inhibition of melanin synthesis and tyrosinase activity by down-regulation of melanogenic enzyme expressions in ${\alpha}$-MSH induced melanogenesis B16/F10 melanoma cells. Results: MKG inhibited tyrosinase-activity, total melanin contents and dendrite out-growth. MKG inhibited melanogenesis by down-regulation of tyorsinase, TRP-1, TRP-2, CREB, and MITF in B16/F10 cells. The treatment with MKG at the 12.5, $25{\mu}g/ml$ level significantly inhibited the melanin synthesis induced ${\alpha}$-MSH in B16/F10 melanoma cells compared with untreated control. Conclusion: These results suggest that MKG inhibit melanin biosynthesis which is involved in hyper-pigmentation. So MKG is considered to be used as a whitening components reducing cytotoxicity.

Keyword

질의응답

핵심어	질문	논문에서 추출한 답변
	멜라닌은 무엇인가?	멜라닌은 피부 표면의 표피 기저층에 존재하는 멜라닌세포의 멜라닌소체에서 합성되어, 수지상 돌기를 통해 표피의 각질형성세포로 이동되어 피부색을 나타내는 색소이고, 자외선에 의해 생성된 oxygen free radical을 제거하여 피부를 보호하는 긍정적인 기능을 하고 있다. 그러나 과도한 멜라닌 생성으로 인해 색소가 침착되어 기미, 주근깨 및 피부반점을 형성하고 피부노화 및 피부암 등 유발에 관여하는 것으로 알려져 있다2,3).
	멜라닌의 과잉 생산은 피부에 어떤 영향을 주는가?	멜라닌은 피부 표면의 표피 기저층에 존재하는 멜라닌세포의 멜라닌소체에서 합성되어, 수지상 돌기를 통해 표피의 각질형성세포로 이동되어 피부색을 나타내는 색소이고, 자외선에 의해 생성된 oxygen free radical을 제거하여 피부를 보호하는 긍정적인 기능을 하고 있다. 그러나 과도한 멜라닌 생성으로 인해 색소가 침착되어 기미, 주근깨 및 피부반점을 형성하고 피부노화 및 피부암 등 유발에 관여하는 것으로 알려져 있다2,3).
	기존에 사용되던 미백물질인 kojic acid, arbutin, vitamin C, 토코페롤 등은 어떤 문제를 가지고 있는가?	화장품 시장에서 사용되는 미백물질은 작용기전에따라 tyrosinase 효소 활성을 억제하는 kojic acid, arbutin과 vitamin C, hydroquinone 등과 자외선차단제나 산란제, 각질층의 턴-오버 촉진, 활성 산소종을 소거하는 토코페롤 등으로 분류할 수 있다. 그러나 이런 물질들은 불안정하여 피부 알러지나 독성을 나타내기도 하며 분해나 착색, 이취, 효과의 불분명 및 안전성 문제 등으로 그 사용이 제한되고 있다6,7). 따라서 안전하면서도 미백활성이 높은 새로운 물질의 개발이 요구되고 있으며, 보다 안전하고 효율적인 천연 한방 성분에 대한 관심이 높아지고 있다.

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