Purpose: We investigated the positivity rate and the time period to the positive color change of the urease test in children and adults and assessed the correlation of the urease test to histopathologic findings. Methods: From 1995 to 2000, endoscopic biopsies of the antrum and body were collected f...
Purpose: We investigated the positivity rate and the time period to the positive color change of the urease test in children and adults and assessed the correlation of the urease test to histopathologic findings. Methods: From 1995 to 2000, endoscopic biopsies of the antrum and body were collected from 811 children and 224 adults and subjected to urease tests and histopathology. Results: The positivity rate of the urease test was 49.4% for 0-4 years, 48.4% for 5-9 years, 47.3% for 10-15 years, and 62.5% for 20-29 years in the antrum. The positivity rate was 85.1% in 0-4 years, 82.3% in 5-9 years, 74.7% in 10-15 years, and 74.1% in 20-29 years for the body. In the antrum, the highest positivity rate was <1 hour for the group aged 10-29 years and 6-24 hours in the group <10 years old (p<0.0001). In the body, the highest positivity rate was <1 hour in adults and 6-24 hours in children (p<0.0001). The proportions of the positive reactions within 1 hour were similar for the antrum and the body. In the cases of more severe chronic gastritis, active gastritis, and Helicobacter pylori infiltration, a positive urease test reaction occurred more quickly (p<0.0001). Conclusion: There were significant differences in urease tests according to age and sampling site. The discrepancy between the antrum and the body was greater in younger children. These results might be related to the low density and patchy distribution of bacteria in children and in the body.
Purpose: We investigated the positivity rate and the time period to the positive color change of the urease test in children and adults and assessed the correlation of the urease test to histopathologic findings. Methods: From 1995 to 2000, endoscopic biopsies of the antrum and body were collected from 811 children and 224 adults and subjected to urease tests and histopathology. Results: The positivity rate of the urease test was 49.4% for 0-4 years, 48.4% for 5-9 years, 47.3% for 10-15 years, and 62.5% for 20-29 years in the antrum. The positivity rate was 85.1% in 0-4 years, 82.3% in 5-9 years, 74.7% in 10-15 years, and 74.1% in 20-29 years for the body. In the antrum, the highest positivity rate was <1 hour for the group aged 10-29 years and 6-24 hours in the group <10 years old (p<0.0001). In the body, the highest positivity rate was <1 hour in adults and 6-24 hours in children (p<0.0001). The proportions of the positive reactions within 1 hour were similar for the antrum and the body. In the cases of more severe chronic gastritis, active gastritis, and Helicobacter pylori infiltration, a positive urease test reaction occurred more quickly (p<0.0001). Conclusion: There were significant differences in urease tests according to age and sampling site. The discrepancy between the antrum and the body was greater in younger children. These results might be related to the low density and patchy distribution of bacteria in children and in the body.
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제안 방법
In this study, we investigated the positivity rate and the positive timing of the urease test in children and adults using three biopsy specimens from the gastric antrum and body and assessed the correlation of the urease test to histopathologic findings.
대상 데이터
After the Institutional Review Board reviewed the research protocols of the present study (GNUHIRB2012-07-003), 811 children and 224 young adults who underwent endoscopy of the upper digestive tract from 1995 to 2000 were enrolled. In children, the most common indication for upper gastrointestinal endoscopy was upper abdominal pain (75.
Histopathologic evaluations were performed on 811 children and 224 adults. The proportion of moderate and severe degrees of chronic gastritis in the antrum increased with age, from 10.
This study was supported by a grant from the National R and D Program for Cancer Control of the Ministry of Health & Welfare of the Republic of Korea (0820050). The biospecimens used in this study were provided by Gyeongsang National University Hospital, which is a member of the National Biobank of Korea that is funded by the Ministry of Health and Welfare.
데이터처리
05 were considered to be statistically significant. The inflammatory changes in the gastric antrum and body regions were correlated with the Spearman rank correlation coefficient.
이론/모형
The relationship in the positive timing of the urease test between the antrum and the body was determined by McNemar’s test.
성능/효과
Another factor related to false-negative urease test was to sampling errors because of a patchy distribution of the organism in the stomach’s mucosa [19]. However, the exact reason of false-negative urease tests in this study could not be proved because this study retrospectively reviewed the results of the urease tests and histology only, and no clinical history of volunteers was evaluated.
The pooled sensitivity of the urease test increased when samples were obtained from both the antrum and the body [13,14]. In this study, the positivity rate of the urease test in both the antrum and the body was higher comparing with only the antrum. Increasing the number of gastric antral biopsies significantly improves the sensitivity of the rapid urease test and hastens the time required for the test to become positive for the diagnosis [11].
pylori in the tissue samples or because of a patchy distribution of the organism in the stomach’s mucosa [11]. In this study, the proportions of the positive color change within 1 hour in the antrum and body were similar in all age groups, and no differences in the speed of the positive reaction in the antrum and the body were observed in the adults. The discrepancy of the urease test between the antrum and the body in children was greater than that in adults, and this outcome resulted from the differences in the proportions of the positive color changes during 6-24 hours.
pylori density in the gastric sample, which is generally lower in children than in adolescents and adults [9]. In this study, the severity of active gastritis and the density of H. pylori in the antrum and body increased with age, and this outcome suggested that the histopathologic findings influence the positivity rate and the positive time in the gastric antrum but not in the gastric body.
참고문헌 (21)
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