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Effect of Extraction Condition on the Content of EGCG and Caffeine of Green Tea: Comparison with the Inhibitory Activity on Pancreatic Lipase 원문보기

Natural product sciences, v.19 no.2, 2013년, pp.166 - 172  

Lee, Eun Song (Gyeonggi Science High School for the Gifted) ,  Lee, Mi Kyeong (College of Pharmacy, Chungbuk National University)

Abstract ▼ AI-Helper 아이콘AI-Helper

Caffeine and epigallocatechin gallate (EGCG) are major constituents of green tea, the leaves of Camellia sinensis (Theaceae). Although EGCG is well known for diverse beneficial effect, caffeine is sometimes harmful with adverse effects. Therefore, the extraction efficiency was investigated using dif...

주제어

#Green tea   #Quantitation   #HPLC-DAD   #Caffeine   #EGCG   #Pancreatic lipase  

AI 본문요약
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제안 방법

  • 0 mg) was weighed accurately and extracted with the mixture of ethanol-water (100, 75, 50, 25 and 0% ethanol in water) for 30 min using sonic apparatus. Effects of extraction time were evaluated by extracting powdered green tea (100.0 mg) with 50% ethanol in water for different time periods such as 5, 10, 20, 60, 120 and 180 min using sonic apparatus. For the optimization of extraction method, powdered green tea was extracted at RT or 70 °C or using ultrasonic apparatus for 30 min.
  • For the optimization of extraction condition with great extraction efficacy and anti-obesity effect, the content of caffeine and EGCG in green tea extraction from different extraction condition was quantitated using HPLC analysis. The inhibitory effect on pancreatic lipase activity was also investigated.
  • General experimental procedures − Analysis was performed using a Waters HPLC system equipped with Waters 600 Q-pumps, a 996 photodiode array detector, and Waters Empower software using Gemini-NX ODScolumn (2.6 µm, 4.6 × 100 mm) for quantitation.
  • The effects of extraction solvent on extraction efficiency were first evaluated. Green tea was extracted with the mixture of ethanol and water (100%, 75%, 50%, 25% and 0% ethanol in water) and analyzed. As shown in Fig.
  • Next, we analyzed the content of EGCG and caffeine in green tea extract using HPLC analysis (Figs. 1A and 1B). The content of EGCG was greatly affected by different extraction condition.

대상 데이터

  • Materials − Dried green tea, the leaves of Camellia sinensis, was provided purchased from the local herbal market, Chungbuk, Korea in November 2012. They were identified by the herbarium of College of Pharmacy at Chungbuk National University, where a voucher specimen was deposited (CBNU201211-GT). Caffeine and EGCG were purchased from Sigma-Aldrich Chemical Co.
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참고문헌 (25)

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