[논문]Effects of Abeliophyllum distichum Nakai Flower Extracts on Antioxidative Activities and Inhibition of DNA Damage

Ahn, Joungjwa; Park, Jae Ho

doi:10.7732/kjpr.2013.26.3.355

Effects of Abeliophyllum distichum Nakai Flower Extracts on Antioxidative Activities and Inhibition of DNA Damage 원문보기

韓國資源植物學會誌 = Korean journal of plant resources, v.26 no.3, 2013년, pp.355 - 361

Ahn, Joungjwa (Department of Food Science and Industry, Jungwon University) , Park, Jae Ho (Department of Medicinal Plant Science, Jungwon University)

Abstract ▼ AI-Helper

The present study attempts to evaluate antioxidant activities of extracts from Abeliophyllum distichum. Nakai flower. The samples were collected in Jangyyeon-myeon, Goesan-gun, Korea and extracted with either hot-water or ethyl acetate (EtOAC). In DPPH, hydroxyl radical scavenging activity and $Fe^{2+}$ chelating activity of EtOAC extracts were 93.41%, 98.43%, and 7.38%, while those of hot-water extracts were 86.93%, 41.33% and 47.68% at 200 ${\mu}g/ml$, respectively. In ${\varphi}X$-174 RF I plasmid DNA cleavage assay, the protective effects of EtOAC and hot-water extracts against oxidative DNA damage were 82% and 17% at 200 ${\mu}g/ml$, respectively. Both extracts showed the protective effect of DNA migration by oxidative stress in intracellular DNA migration assay. Both extracts had no cytotoxity in NIH3T3 cells. Several polyphenolic compounds were identified such as 2-methoxy-benzoic acid, vanillic acid, phytol and pulegone by GC/MS. These results indicated that extracts of Abeliophyllum distichum Nakai flower showed antioxidant activities and protective activities against oxidative DNA damage and showed the possibility to be used as an effective natural antioxidants.

주제어

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문제 정의

Therefore, in this study, we demonstrate the protective effect on oxidative DNA damage of extracts from Abeliophyllum distichum Nakai via its antioxidant activity for the establishment of new value for the herbal medicine.

제안 방법

Earlier study in our lab has been shown antioxidant activities and protective effect of Abeliophyllum distichum Nakai leaves and possibility of its application (Park, 2011). Therefore, the present study was designed to evaluate the inhibitory effect on oxidative DNA damage of extracts from Abeliophyllum distichum Nakai flower by analyzing its antioxidant activities and cytotoxity.

대상 데이터

The major 10 compounds were identified and listed by GC/MS (Fig. 7). Those were polyphenolic compounds such as coumaran, bensenacetic acid and vanllic acid, which may be related with antioxidant activities of Abeliophyllum distichum Nakai flower extracts.

성능/효과

The results indicated that no inhibitory effect was found in low concentrations of both aqueous and EtOAC fractions, whereas little effect was found at 200 μg/ml (Fig. 5).
Therefore, these results indicated that the extracts from Abeliophyllum distichum Nakai flower had high oxidative activities and protective effect on DNA damage and showed the possible use of Abeliophyllum distichum Nakai flower as a effective natural antioxidants.

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