사물탕가향부자(四物湯加香附子)가 난소적출 흰쥐의 우울 및 학습에 미치는 영향 The Effect of Samul-tanggahyangbuja on Depression and Learning on Repeated Stress in Ovariectomized Rats원문보기
목 적: 본 연구에서는 부인과에서 빈용되고 있는 사물탕에 향부자를 가미한 처방을 사용하여 반복적인 스트레스를 가한 난소적출 흰쥐에 대하여 항우울 효과와 학습에 미치는 영향을 살펴보고자 하였다. 방 법: 난소적출 흰쥐에 2주간 반복적인 스트레스를 주고, 사물탕가향부자(100 mg/kg, 400 mg/kg)을 경구 투여한 후 행동검사로 Morris water maze test, Forced swimming test, Sucrose intake 측정과 Social exploration 관찰을 시행하였으며 혈액검사로 혈청 Corticosterone, IL-$1{\beta}$와 TNF-${\alpha}$의 변화를 측정하였다. 결 과: 1. Morris water maze test에서 사물탕가향부자 400 mg 투여군은 실험 3, 4일째 에 사물탕가향부자 100 mg 투여군은 실험 4일째에 acquisition trial 수행의 시간이 대조군에 비해서 단축되는 유의한 효과를 보였다. 2. Forced swimming test에서 사물탕가향부자 400 mg 투여군은 대조군에 비해서 immobility 시간이 유의하게 줄어들었다. 3. Sucrose intake test에서 사물탕가향부자 400 mg 투여군은 대조군에 비해서 현저히 자당 섭취량이 증가하였고, social exploration 관찰에서 사물탕가향부자 400 mg 투여군은 대조군에 비해서 현저히 active social behavior가 증가하였다. 4. Corticosterone 측정에서 사물탕가향부자를 투여한 후 corticosterone 수준이 감소하는 변화가 있었지만 유효하지는 않았다. 5. 사물탕가향부자 투여군(100 mg, 400 mg)에서 IL-$1{\beta}$와 TNF-${\alpha}$의 양이 대조군에 비해 유의하게 감소되었다. 결 론: 이상의 결과로 사물탕가향부자는 난소적출 흰쥐의 우울 및 인지력 저하에 유효함을 알 수 있었다.
목 적: 본 연구에서는 부인과에서 빈용되고 있는 사물탕에 향부자를 가미한 처방을 사용하여 반복적인 스트레스를 가한 난소적출 흰쥐에 대하여 항우울 효과와 학습에 미치는 영향을 살펴보고자 하였다. 방 법: 난소적출 흰쥐에 2주간 반복적인 스트레스를 주고, 사물탕가향부자(100 mg/kg, 400 mg/kg)을 경구 투여한 후 행동검사로 Morris water maze test, Forced swimming test, Sucrose intake 측정과 Social exploration 관찰을 시행하였으며 혈액검사로 혈청 Corticosterone, IL-$1{\beta}$와 TNF-${\alpha}$의 변화를 측정하였다. 결 과: 1. Morris water maze test에서 사물탕가향부자 400 mg 투여군은 실험 3, 4일째 에 사물탕가향부자 100 mg 투여군은 실험 4일째에 acquisition trial 수행의 시간이 대조군에 비해서 단축되는 유의한 효과를 보였다. 2. Forced swimming test에서 사물탕가향부자 400 mg 투여군은 대조군에 비해서 immobility 시간이 유의하게 줄어들었다. 3. Sucrose intake test에서 사물탕가향부자 400 mg 투여군은 대조군에 비해서 현저히 자당 섭취량이 증가하였고, social exploration 관찰에서 사물탕가향부자 400 mg 투여군은 대조군에 비해서 현저히 active social behavior가 증가하였다. 4. Corticosterone 측정에서 사물탕가향부자를 투여한 후 corticosterone 수준이 감소하는 변화가 있었지만 유효하지는 않았다. 5. 사물탕가향부자 투여군(100 mg, 400 mg)에서 IL-$1{\beta}$와 TNF-${\alpha}$의 양이 대조군에 비해 유의하게 감소되었다. 결 론: 이상의 결과로 사물탕가향부자는 난소적출 흰쥐의 우울 및 인지력 저하에 유효함을 알 수 있었다.
Objectives: In this research, the effect of samul-tanggahyangbuja on depression and learning in ovariectomized rats subjected to repetitive stress were assessed. Samul-tanggahyangbuja is the prescription consisting of Samul-tang and Cyperi Rhizoma. Methods: Ovariectomized rats were repeatedly stress...
Objectives: In this research, the effect of samul-tanggahyangbuja on depression and learning in ovariectomized rats subjected to repetitive stress were assessed. Samul-tanggahyangbuja is the prescription consisting of Samul-tang and Cyperi Rhizoma. Methods: Ovariectomized rats were repeatedly stressed over a 2-week period. After being orally medicated with samul-tanggahyangbuja (100 or 400 mg/kg), rats performed the Morris water maze test and forced swimming test, and social exploration was assessed in a behavior test. As well, sucrose intake was measured and measurements of blood serum corticosterone and the change of interleukin-$1{\beta}$ (IL-$1{\beta}$) and tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$) in blood samples were made. Results: 1. In the Morris water maze test, rats medicated with 100 mg samul-tanggahyangbuja mastered the maze in a shorter time on the 4th day in comparison with the control group, while rats medicated with 400 mg samul-tanggahyangbuja mastered the maze more quickly (p<0.05 on the 3rd day ; p<0.01 on the 4th day, as compared to control). 2. Immobility time in the forced swimming test was significantly decreased in rats receiving 400 mg samul-tanggahyangbuja compared with the control group (p<0.05). 3. Sucrose intake and active social behavior of rats receiving 400 mg samul-tanggahyangbuja were markedly increased in comparison with the control group (p<0.01). 4. Blood serum corticosterone measurements revealed decreased blood serum corticosterone level after medicating with samul-tanggahyangbuja. But it was not statistically significant. 5. Treatment with either dose of samul-tanggahyangbuja significantly reduced IL-$1{\beta}$ and TNF-${\alpha}$ (p<0.05). Conclusions: These results suggest that samul-tanggahyangbuja possesses the anti-depressant and cognitive-enhancing activities related to menopause.
Objectives: In this research, the effect of samul-tanggahyangbuja on depression and learning in ovariectomized rats subjected to repetitive stress were assessed. Samul-tanggahyangbuja is the prescription consisting of Samul-tang and Cyperi Rhizoma. Methods: Ovariectomized rats were repeatedly stressed over a 2-week period. After being orally medicated with samul-tanggahyangbuja (100 or 400 mg/kg), rats performed the Morris water maze test and forced swimming test, and social exploration was assessed in a behavior test. As well, sucrose intake was measured and measurements of blood serum corticosterone and the change of interleukin-$1{\beta}$ (IL-$1{\beta}$) and tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$) in blood samples were made. Results: 1. In the Morris water maze test, rats medicated with 100 mg samul-tanggahyangbuja mastered the maze in a shorter time on the 4th day in comparison with the control group, while rats medicated with 400 mg samul-tanggahyangbuja mastered the maze more quickly (p<0.05 on the 3rd day ; p<0.01 on the 4th day, as compared to control). 2. Immobility time in the forced swimming test was significantly decreased in rats receiving 400 mg samul-tanggahyangbuja compared with the control group (p<0.05). 3. Sucrose intake and active social behavior of rats receiving 400 mg samul-tanggahyangbuja were markedly increased in comparison with the control group (p<0.01). 4. Blood serum corticosterone measurements revealed decreased blood serum corticosterone level after medicating with samul-tanggahyangbuja. But it was not statistically significant. 5. Treatment with either dose of samul-tanggahyangbuja significantly reduced IL-$1{\beta}$ and TNF-${\alpha}$ (p<0.05). Conclusions: These results suggest that samul-tanggahyangbuja possesses the anti-depressant and cognitive-enhancing activities related to menopause.
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